LOCUS       sequence-353639-        8653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0203
FEATURES             Location/Qualifiers
     source          1..8653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1591..2179
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1644..1664)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2062..2073)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2251..2484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2562..2683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2599..2618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2653..2672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2723..2858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2878..2897)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2879..2897)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2907..2924)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2907..2923)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2916..2938)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3065..3520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3152..3171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3362..3383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3546..3650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3651..3719,3720..4511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3869..3888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4682..5270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5171..5190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5424..5441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5558..5579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5594..5599,5600..5617,5618..5624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5632..5648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5637..5659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5656..5672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5656..5672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5691..5711
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5693..5711
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5739..5965
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5966..6146
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6193..6318
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6811..7044
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7229..7273
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7504..7714
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7526..7722
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7573..7708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7754..8356
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7754..7773)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8250..8270
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8399..8516
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 cccatctgtg acgctgtggc agtttctgct gcagctgctg agagagcaag gcaatggcca
     1261 catcatctcc tggacttcac gggatggtgg tgaattcaag ctggtggatg cagaggaggt
     1321 ggcccggctg tgggggctac gcaagaacaa gaccaacatg aattacgaca agctcagccg
     1381 ggccttgcgg tactactatg acaagaacat catccgcaag gtgagcggcc agaagttcgt
     1441 ctacaagttt gtgtcctacc ctgagtccca ttgcgccccg tagtaatgaa ctagtccgta
     1501 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg agtccaaggc
     1561 tcaccgcaca cccagacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     1621 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     1681 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     1741 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     1801 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     1861 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     1921 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     1981 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2041 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2101 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     2161 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     2221 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     2281 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2461 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     2521 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     2581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     2701 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     2761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     2821 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     2881 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     2941 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3001 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3061 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3121 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3181 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3241 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3301 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3361 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3421 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3481 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     3541 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3601 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3661 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3721 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3781 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3841 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3901 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3961 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4021 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4081 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4141 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4201 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4261 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4321 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4381 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4441 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4501 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4561 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5341 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5401 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5461 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5521 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5581 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5641 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     5701 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     5761 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     5821 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     5881 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     5941 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     6001 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6061 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6121 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     6181 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     6241 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     6301 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     6361 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     6421 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     6481 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     6541 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     6601 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     6661 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     6721 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     6781 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     6841 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     6901 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     6961 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7021 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7081 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7141 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7201 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     7261 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     7321 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7381 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7441 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7501 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     7561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7621 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7681 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     7741 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     7801 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7861 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     7921 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     7981 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8041 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8101 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     8161 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     8221 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     8281 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     8341 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     8401 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     8461 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     8521 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     8581 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     8641 aggagtggga att
//
