LOCUS       sequence-353641-        1703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 6 containing non-coding DNA 
            spacer for construction of MoClo plasmids without yeast marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC6-spacer
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(142..176)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(247..906)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     821..840
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1088..1676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1577..1596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
       61 gtggataacc gtagtcggtc tcatacaact agagatctat gtgaggatcc taactcgaga
      121 tcgagagttg agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      181 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
      241 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      301 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
      361 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
      421 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
      481 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
      541 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      601 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
      661 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
      721 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
      781 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
      841 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      901 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      961 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
     1021 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1081 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1141 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1201 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1261 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1321 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1381 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1441 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1501 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1561 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1621 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1681 ccagcaacgc ggccttttta cgg
//
