LOCUS       sequence-353650-       16301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and 
            Basta for fast transformant selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16301)
  AUTHORS   Bieluszewski T, Sura W, Dziegielewski W, Bieluszewska A, Lachance C,
            Kabza M, Szymanska-Lejman M, Abram M, Wlodzimierz P, De Winne N, De 
            Jaeger G, Sadowski J, Cote J, Ziolkowski PA
  TITLE     NuA4 and H2A.Z control environmental responses and autotrophic 
            growth in Arabidopsis.
  JOURNAL   Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5.
  PUBMED    35022409
REFERENCE   2  (bases 1 to 16301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-12"; volume: "13(1):277. doi"; pages: " 
            10.1038/s41467-021-27882-5"
COMMENT     Sequence Label: pFGC-I2Cas9
FEATURES             Location/Qualifiers
     source          1..16301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2080..2220
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2146..2165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2306..2328
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2564..2758
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2824..3891)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(4325..4954)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6254..6278
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6466..6488
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6480..6497
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6481..6497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8324..8389
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8396..8416
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8441..12541
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8441..12541
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             12542..12589
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      12628..12880
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      14721..14973
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        14992..15372
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             15378..15929
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      15939..16191
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    16277..16301
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2101 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2161 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     2221 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2281 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2341 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     2401 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     2461 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     2521 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     2581 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     2641 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     2701 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     2761 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     2821 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     2881 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     2941 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     3001 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     3061 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     3121 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     3181 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     3241 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     3301 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     3361 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     3421 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     3481 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     3541 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     3601 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     3661 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     3721 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     3781 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     3841 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg
     3901 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     3961 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     4021 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     4081 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     4141 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     4201 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     4261 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     4321 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     4381 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     4441 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     4501 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     4561 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     4621 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     4681 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     4741 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     4801 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     4861 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     4921 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     4981 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     5041 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     5101 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     5161 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     5221 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     5281 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
     5341 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
     5401 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
     5461 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
     5521 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
     5581 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
     5641 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
     5701 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
     5761 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
     5821 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
     5881 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
     5941 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
     6001 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
     6061 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
     6121 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc
     6241 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa
     6301 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggccagtgcc aagctcaacg ctgccagatt cgatgtaagt tactggaaac
     6541 tgaaccgaag ggaactctcc aggaaacaga gatggaaaac tgaaactaac tctgtttcca
     6601 aacgttaggg ttccttgaaa ttgcctacca attgttcccg ctccaaccgt cataatccac
     6661 ggcgctccgt taatcaaact ccagtaatat ggcccgtcgt taccgccgga agtaaccacg
     6721 aaaacgcctt tttgaatcgc tgcaaaagat gcgactgcga ttggatcatt ttctagaccg
     6781 aaaccatcat tgtcgtcatc atcctcaaaa ctcaatccta gcgacagaga tatcacatgg
     6841 actccatctc gaatcgcttg atcaatcgct gcgattacat ctgatgagta aatcccttct
     6901 tcccacgcgg ctttgtagat tgctagatgc gcgtgtggtg cgattcctga ggcggttcct
     6961 tgggcgtaag agaagtaaga cgcgttctta acatgattcc cagctgcaat ggctgcaaca
     7021 tgagtgccgt gtccgattgt atcgtacggt gaagagtatt ggccaatctt ggtttctctc
     7081 aaatcagggt tgttggcgaa caaacccttg ttgaatacct tagcaccgat tagtttcttg
     7141 ttgcacaagg aagaagaatt gaattcacat gctcctttcc atttagacgg aaccgaacca
     7201 accccatcgt cgtgaaagct gggactatcg ggccaaattc cggtatctat aataccgatt
     7261 actataccag ctccataatt cgagaccggc catgtaccgg atgtcgaatt taaaccgata
     7321 aactttggag agaaagtggt atgaagctta accggtaaat ctttggtgaa cgagacatac
     7381 ccgggtttgt gtttaagacg ttggagctca gagttggtga gaaccgcgct aaacccgtga
     7441 accgaatcgg tataagcgta gataatcttt ggttttctat tggttataac tgaagtaaga
     7501 gttgtggaaa accagctccg atgatcggaa aatggcaacg gtttagcgga taagtccatg
     7561 tggatgatgt aaggagaggt ctccgccgtt acaatcgcta cgaaaaatga gaacaccaga
     7621 ataattacga cggtcatccc catgtctgag caagtttttt tctctggtct cttcaaagtg
     7681 aaatgaattt tggtgttagt aagaaacaat tatagtgttg ttgttaatag atgtttagtt
     7741 tttattatga caaaaaagtt gcagagaatg taacctttac ttggcttttg cttttgttaa
     7801 agtggtttta ggtggtcggc aacacatcat ctctcatcat tgtttttttt tcttttctct
     7861 ttcaattggg ctttatcata gagaacaatg attttggata tgaacttatt agtagtttca
     7921 gcaatagttt agactttcga atcggtaaat atcagtaact tagaactcag gctgaaactt
     7981 tgattctttt actgtattta gctagattct acacttggtg gactccatca gcaattactt
     8041 cacagaccga ggtagctcct acaactctct gatgcttgat ctcgttcaaa tagatgtctc
     8101 ataattgctt cttttttgtt tttgtttttg ggctttatta aagaggagta aggcccatta
     8161 agagaacccg tttgtttgtt ggattcggat ttggcaaatg gatagcgcca aaatcgtgtc
     8221 gattttcccg ccatctccat ttcttaatct gggttcttaa acctaaatca gttactcagc
     8281 ttcaccaaac caccaaattg accggatttg taaaatcgag atggactata aggaccacga
     8341 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
     8401 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
     8461 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     8521 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     8581 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     8641 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     8701 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     8761 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     8821 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     8881 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     8941 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     9001 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     9061 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     9121 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     9181 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     9241 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     9301 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     9361 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     9421 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     9481 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     9541 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     9601 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     9661 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     9721 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     9781 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     9841 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     9901 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     9961 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
    10021 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
    10081 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
    10141 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
    10201 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
    10261 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
    10321 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
    10381 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
    10441 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
    10501 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
    10561 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
    10621 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
    10681 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
    10741 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
    10801 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
    10861 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
    10921 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
    10981 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
    11041 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
    11101 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
    11161 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
    11221 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
    11281 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
    11341 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
    11401 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
    11461 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
    11521 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
    11581 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
    11641 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
    11701 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
    11761 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
    11821 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
    11881 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
    11941 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
    12001 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
    12061 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
    12121 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
    12181 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
    12241 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
    12301 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
    12361 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
    12421 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
    12481 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
    12541 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagt aaggatcttg
    12601 attgatcgat agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
    12661 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    12721 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    12781 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    12841 gataaattat cgcgcgcggt gtcatctatg ttactagatc ggggatcctt ccatgggaat
    12901 tctgagctct cttcatcggt gattgattcc tttaaagact tatgtttctt atcttgcttc
    12961 tgaggcaagt attcagttac cagttaccac ttatattctg actttctgac tgcatcctca
    13021 tttttccaac attttaaatt tcatattggc tgaatgcttc ttctttgagg aagaaacaat
    13081 tcagatggca gaaatgtatc aaccaatgca tatatacaaa tgtacctctt gttctcaaaa
    13141 catctatcgg atggttccat ttgctttgtc atccaattag tgactacttt atattattca
    13201 ctccttttta ttactatttc atggaggttg ccatgtacat tatatttgta aggatgacgc
    13261 tattgagcgt ttttcttcaa ttttctttat tttagacatg ggtatgaaat gtgtgttaga
    13321 gttgggttga atgagatata cgttcaagtg aagtggcata ccgttgtcga gtaaggatga
    13381 cctacccatt cttgagacaa atgttacatt ttagtatcag agtaaaatgt gtacctataa
    13441 ctcaaattcg attgacatgt atccattcaa cataaaatta aaccagcctg cacctgcatc
    13501 cacatttcaa gtattttcaa accgttcggc tcctatccac cgggtgtaac aagacggatt
    13561 ccgaatttgg aagattttga ctcaaattcc caatttatat tgaccgtgac taaatcaact
    13621 ttaacttcta taattctgat taagctccca atttatattc ccaacggcac tacctccaaa
    13681 atttatagac tctcatcccc ttttaaacca acttagtaaa cgctcttttt tttaatttta
    13741 tgaagttaag tttttacctt gtttttaaaa agaatcgttc ataagatgcc atgccagaac
    13801 attagctaca cgttacacat agcatgcagc cgcggagaat tgtttttctt cgccacttgt
    13861 cactcccttc aaacacctaa gagcttctct ctcacagcac acacatacaa tcacatgcgt
    13921 gcatgcatta ttacacgtga tcgccatgca aatctccttt atagtctata aattaactca
    13981 tccgcttcac tctttactca aaccaaaact catcaataca aacaagatta aaaacatacc
    14041 ccatgcggtc ttccaagaat gttatcaagg agttcatgag gtttaaggtt cgcatggaag
    14101 gaacggtcaa tgggcacgag tttgaaatag aaggcgaagg agaggggagg ccatacgaag
    14161 gccacaatac cgtaaagctt aaggtaacca aggggggacc tttgccattt gcttgggata
    14221 ttttgtcacc acaatttcag tatggaagca aggtatatgt caagcaccct gccgacatac
    14281 cagactataa aaagctgtca tttcctgaag gatttaaatg ggaaagggtc atgaactttg
    14341 aagacggtgg cgtcgttact gtaacccagg attccagttt gcaggatggc tgtttcatct
    14401 acaaggtcaa gttcattggc gtgaactttc cttccgatgg acctgttatg caaaagaaga
    14461 caatgggctg ggaagccagc actgagcgtt tgtatcctcg tgatggcgtg ttgaaaggag
    14521 agattcataa ggctctgaag ctgaaaaacg gtggtcatta cctagttgaa ttcaaaagta
    14581 tttacatggc aaagaagcct gtgcagctac cagggtacta ctatgttgac tccaaactgg
    14641 atataacaag ccacaacgaa gactatacaa tcgttgagca gtatgaaaga accgagggac
    14701 gccaccatct gttcctttaa gatcgttcaa acatttggca ataaagtttc ttaagattga
    14761 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
    14821 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
    14881 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
    14941 tatcgcgcgc ggtgtcatct atgttactag atcgcatggg aattctgaga tttttcaaat
    15001 cagtgcgcaa gacgtgacgt aagtatctga gctagttttt atttttctac taatttggtc
    15061 gtttatttcg gcgtgtagga catggcaacc gggcctgaat ttcgcgggta ttctgtttct
    15121 attccaactt tttcttgatc cgcagccatt aacgactttt gaatagatac gctgacacgc
    15181 caagcctcgc tagtcaaaag tgtaccaaac aacgctttac agcaagaacg gaatgcgcgt
    15241 gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga taaatagcct tgcttcctat
    15301 tatatcttcc caaattacca atacattaca ctagcatctg aatttcataa ccaatctcga
    15361 tacaccaaat cgaatcgatg agcccagaac gacgcccggc cgacatccgc cgtgccaccg
    15421 aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca
    15481 acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg
    15541 agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg
    15601 gcccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc
    15661 cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg
    15721 aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc
    15781 gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc
    15841 acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg gtaccgcccc
    15901 gtccggtcct gcccgtcacc gagatctaac tgcagaatct tggactccca tgttggcaaa
    15961 ggcaaccaaa caaacaatga atgatccgct cctgcatatg gggcggtttg agtatttcaa
    16021 ctgccatttg ggctgaattg aagacatgct cctgtcagaa attccgtgat cttactcaat
    16081 attcagtaat ctcggccaat atcctaaatg tgcgtggctt tatctgtctt tgtattgttt
    16141 catcaattca tgtaacgttt gcttttctta tgaattttca aataaattat cagatccgag
    16201 ctcgaattaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
    16261 gtctaagcgt caatttgttt acaccacaat atatcctgcc a
//
