LOCUS       sequence-353671-        6645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV 
            production vector for expression in excitatory glutamatergic neurons
            using the promoter CaMKII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, 
            Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, 
            Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, 
            Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias
            AS, Reimer J, Dieudonne S, St-Pierre F
  TITLE     Sustained deep-tissue voltage recording using a fast indicator 
            evolved for two-photon microscopy.
  JOURNAL   Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013.
  PUBMED    35985322
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013."
COMMENT     Sequence Label: pAAV-CaMKIIa-JEDI-2P-WPRE
FEATURES             Location/Qualifiers
     source          1..6645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      145..733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     634..653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   795..924
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        943..2231
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      2252..2261
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3566..4154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3619..3639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4037..4048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4186..4662
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4385..4404)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4702..4842
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4702..4831
                     /label=AAV2 ITR
     rep_origin      4917..5372
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5004..5023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5214..5235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5389..5408)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5508..5530
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5568..5586)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5654..5758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5759..5827,5828..6619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5977..5996)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
       61 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      781 ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc
      841 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
      901 ccaactccat cactaggggt tcctgcggcc gcacgcgttt aacattatgg ccttaggtca
      961 cttcatctcc atggggttct tcttctgatt ttctagaaaa tgagatgggg gtgcagagag
     1021 cttcctcagt gacctgccca gggtcacatc agaaatgtca gagctagaac ttgaactcag
     1081 attactaatc ttaaattcca tgccttgggg gcatgcaagt acgatataca gaaggagtga
     1141 actcattagg gcagatgacc aatgagttta ggaaagaaga gtccagggca gggtacatct
     1201 acaccacccg cccagccctg ggtgagtcca gccacgttca cctcattata gttgcctctc
     1261 tccagtccta ccttgacggg aagcacaagc agaaactggg acaggagccc caggagacca
     1321 aatcttcatg gtccctctgg gaggatgggt ggggagagct gtggcagagg cctcaggagg
     1381 ggccctgctg ctcagtggtg acagataggg gtgagaaagc agacagagtc attccgtcag
     1441 cattctgggt ctgtttggta cttcttctca cgctaaggtg gcggtgtgat atgcacaatg
     1501 gctaaaaagc agggagagct ggaaagaaac aaggacagag acagaggcca agtcaaccag
     1561 accaattccc agaggaagca aagaaaccat tacagagact acaaggggga agggaaggag
     1621 agatgaatta gcttcccctg taaaccttag aacccagctg ttgccagggc aacggggcaa
     1681 tacctgtctc ttcagaggag atgaagttgc cagggtaact acatcctgtc tttctcaagg
     1741 accatcccag aatgtggcac ccactagccg ttaccatagc aactgcctct ttgccccact
     1801 taatcccatc ccgtctgtta aaagggccct atagttggag gtgggggagg taggaagagc
     1861 gatgatcact tgtggactaa gtttgttcgc atccccttct ccaaccccct cagtacatca
     1921 ccctggggga acagggtcca cttgctcctg ggcccacaca gtcctgcagt attgtgtata
     1981 taaggccagg gcaaagagga gcaggtttta aagtgaaagg caggcaggtg ttggggaggc
     2041 agttaccggg gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc
     2101 tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg
     2161 tcagtcaagc cggttctccg tttgcactca ggagcacggg caggcgagtg gcccctagtt
     2221 ctgggggcag ctctagagcg gtaccggatc cgccaccatg gagacgactg tgaggtatga
     2281 acaggggtca gagctcacta aaacttcgag ctctccaaca gcagatgagc ccacgataaa
     2341 gattgatgat ggtcgtgatg agggtaatga acaagacagc tgttccaata ccattaggag
     2401 aaaaatttcc ccgtttgtga tgtcatttgg attcagagta tttggagttg tgcttatcat
     2461 tgtagacatc atagtggtga ttgtggatct ggccatcagt gagaagaaaa gaggcattag
     2521 agagattctt gaaggtgttt ccctggctat agcactcttc ttccttgttg atgttctcat
     2581 gagagtgttt gttgaaggct tcaagaacta tttccggtcc aaactggtta ctttggatgc
     2641 agtcatagta gtgggcactc tgctaattaa tatgacctac tccttctctg accttgctgc
     2701 ctttaacagc gagaacgtgt atattaccgc ggataaacag aaaaacggca ttaaagcgaa
     2761 ctttaccgtg cgccataacg tggaagatgg cagcgtgcag ctggcggatc attatcagca
     2821 gaacaccccg attggcgatg gcccggtgct gctgccggat aaccattatc tgagccacca
     2881 gaccgtgctg agcaaagatc cgaacgaaaa acgcgatcac atggtgctgc tggaatttgt
     2941 gaccgcagcg ggcattacac acggcatgga tgaactgtat ggcggcaccg gcggcagcgc
     3001 gagccagggc gaagaactgt ttaccggcgt ggtgccgatt ctggtggaac tggatggcga
     3061 tgtgaacggc cataaattta gcgtgcgcgg cgaaggcgaa ggcgatgcga ccattggcaa
     3121 actgaccctg aaatttattt gcaccaccgg caaactaccg gtgccgtggc cgaccctggt
     3181 gaccacctta acctatggcg tgcagtgctt tagccgctat ccggatcata tgaaacgcca
     3241 tgattttttt aaaagcgcga tgccggaagg ctatgtgcag gaacgcacca ttagctttaa
     3301 agatgatggc aaatataaaa cccgcgcggt ggtgaaattt gaaggcgata ccctggtgaa
     3361 ccgcattgaa ctgaaaggca ccgattttaa agaagatggc aacattctgg ggcataaact
     3421 ggaatatgat acagatcaga tgccgcacat ggttactctt cttcgagttc tgaaaattgt
     3481 tatcttaata agaatatttc gcctggcttc acagaagaaa caacttgaag tggtaaccta
     3541 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     3601 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3661 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3721 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3781 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3841 cggaactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3901 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3961 atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc
     4021 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4081 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4141 ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt
     4201 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     4261 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     4321 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     4381 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     4441 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     4501 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     4561 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     4621 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
     4681 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4741 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4801 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4861 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4921 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4981 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5041 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5101 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     5161 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5221 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     5281 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5341 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     5401 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5461 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5521 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6001 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6301 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6601 actgattaag cattggtaac tgtcagacca agtttactca tatat
//
