LOCUS       sequence-353678-       10814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence
            codon-optimized..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, 
            Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, Christensen MH, 
            Conzelmann C, Muller JA, Srinivasachar Badarinarayan S, Sturzel CM, 
            Forne I, Stenger S, Conzelmann KK, Munch J, Schmidt FI, Sauter D, 
            Imhof A, Kirchhoff F, Sparrer KMJ
  TITLE     Systematic functional analysis of SARS-CoV-2 proteins uncovers viral
            innate immune antagonists and remaining vulnerabilities.
  JOURNAL   Cell Rep. 2021 May 18;35(7):109126.
  PUBMED    33974846
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-05-18"; volume: "35(7)"; pages: "109126"
COMMENT     Sequence Label: pCG_SARS-CoV1 NSP3 C-V5-tag
FEATURES             Location/Qualifiers
     source          1..10814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     regulatory      667..676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6442..6483
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          6513..7085
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(7217..7236)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7282..7337
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7336..7355)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      7691..7826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7932..7949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8103..8691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8183..8202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8862..9653,9654..9722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9485..9504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9723..9827)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9895..9913
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9951..9973)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10073..10092
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(10159..10614)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10296..10317)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10508..10527
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10740..10762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10754..10771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10755..10771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10778..10797
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10778..10796
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtaggcct
      661 tctagagcca ccatggcccc cattaagggc gtgaccttcg gcgaggacac cgtgtgggag
      721 gtgcagggct ataagaatgt gagaatcaca tttgagctgg atgagagagt ggataaagtg
      781 ctgaatgaga aatgtagcgt gtacaccgtg gagtctggaa cagaggtgac agagttcgcc
      841 tgtgtggtgg ctgaggccgt ggttaagaca ctgcagccag tgtctgacct gctgacaaac
      901 atgggcatcg atctggatga gtggagtgtg gctacatttt acctgtttga tgacgctggc
      961 gaggagaatt ttagctctag gatgtactgt agcttctacc cccctgatga agaagaagaa
     1021 gatgatgctg agtgtgaaga agaggagatc gatgagacct gcgagcacga gtacggcacc
     1081 gaggacgact accagggcct gcccctggag ttcggcgcca gcgccgagac cgtgagggtg
     1141 gaggaagagg aggaggagga ctggctggac gacaccaccg agcagtccga gatagagccc
     1201 gagcccgagc caacgcctga ggagccagtg aaccagttca ccggctacct gaagctgacc
     1261 gacaacgtgg ccattaagtg cgtggacata gtgaaggagg cccagagcgc caaccctatg
     1321 gtcatcgtga atgccgccaa catccacctg aagcacggcg gcggcgtggc cggcgccctg
     1381 aacaaggcca ccaacggcgc tatgcagaag gagagcgacg actacatcaa gctgaatggc
     1441 cccctgaccg tgggcggcag ctgcctgctg agcggccaca acctggccaa gaagtgcctg
     1501 cacgtggtgg gccccaacct gaacgccggc gaggacatcc agctgctgaa ggccgcctac
     1561 gagaacttca actcccagga catcctgctg gcccccctgc tgtccgccgg catctttggg
     1621 gccaagcccc tgcagagcct gcaggtgtgc gtgcagaccg tgagaaccca ggtgtacatc
     1681 gccgtgaatg acaaggccct gtacgagcag gtggtcatgg actacctgga caacctgaag
     1741 cctagggtgg aggcccccaa gcaggaggag ccccccaaca ccgaggacag caagaccgag
     1801 gagaagtccg tggtgcagaa gcccgtggac gtgaagccca aaatcaaggc ctgtattgac
     1861 gaggtgacca ccaccctgga ggagaccaag ttcctgacca ataagctgct gctgttcgcc
     1921 gacatcaacg gcaagctgta ccacgatagc cagaacatgc tgaggggcga ggacatgagc
     1981 ttcctggaga aagacgcccc ctacatggtg ggcgacgtga tcaccagcgg cgacatcacc
     2041 tgcgtcgtga tccctagcaa gaaggccgga ggaacaacag aaatgcttag tagagcactg
     2101 aagaaggtgc ctgtggatga gtatattaca acatatccag gtcagggatg tgccggatac
     2161 acactggagg aagctaagac cgccctgaaa aagtgtaaat ccgcctttta cgtgctgcca
     2221 tctgaggcgc ctaacgctaa agaagagatc ctgggaaccg tgtcctggaa cctgagagag
     2281 atgctggccc acgccgagga gacccggaag ctgatgccca tctgcatgga cgtgcgggcc
     2341 atcatggcca ccatccagag aaagtacaag ggcattaaga tccaggaggg catcgtggac
     2401 tacggcgtga gatttttctt ctacacctcc aaggagcccg tggcctccat catcaccaag
     2461 ctgaatagcc tgaacgagcc cctggtgacc atgcccatcg gctacgtgac ccacggcttc
     2521 aacctggagg aggccgccag atgcatgaga agcctgaagg cccctgccgt ggtgagcgtg
     2581 agcagccccg acgccgtgac cacctacaac ggatacctaa cctccagcag caagacctcc
     2641 gaggagcact tcgtggagac cgtgagcctg gccggcagct acagggactg gagctactcc
     2701 ggccagagga ccgagctggg cgtggagttt ctgaagaggg gcgataagat cgtgtaccat
     2761 accctggaga gccccgtgga gttccacctg gacggcgagg tgctgagcct ggacaagctg
     2821 aagtccctgc tgagcctgcg cgaggtcaag accatcaagg tgtttacaac tgtggataat
     2881 accaatctgc acacacagct ggtggatatg tctatgactt atggccagca gttcggacca
     2941 acatatctgg atggcgcgga tgtgactaag ataaaaccac acgtgaatca tgaaggaaag
     3001 acctttttcg tgctgccctc cgatgatacc ctgaggtccg aagcctttga gtactaccac
     3061 accctggacg agagcttcct gggaaggtac atgagcgccc tgaaccacac caagaagtgg
     3121 aagttccccc aggtgggggg cctgaccagc attaagtggg ccgacaacaa ctgctacctg
     3181 agcagcgtgc tgctggccct gcaacagctg gaggtgaagt tcaacgcccc cgccctgcag
     3241 gaggcctact accgggccag agccggcgat gctgccaatt tctgtgccct gatcctggcc
     3301 tactctaaca agaccgttgg cgagctgggc gacgtgaggg agaccatgac ccatctgctg
     3361 cagcatgcta atctggagtc tgccaagaga gtgctgaatg tggtgtgcaa gcattgtggc
     3421 cagaaaacca ccaccctgac cggcgtggag gccgtgatgt acatgggcac cctgagctat
     3481 gataatctca aaaccggagt gagtattccc tgcgtgtgtg gcagggatgc cacacagtac
     3541 ctggtgcagc aggagagcag ctttgtgatg atgagcgccc ctcctgccga gtataaactg
     3601 cagcagggca catttctgtg cgccaatgaa tacaccggta actaccagtg cggacactac
     3661 acacacatca ccgccaagga gaccctgtat agaattgatg gcgctcacct gacaaagatg
     3721 tctgagtata agggcccagt gactgatgtg ttttataaag agacttccta cacaacaaca
     3781 attaagccag tgtcctataa gctggatgga gtgacctaca ccgaaattga gccaaaactg
     3841 gatggctatt acaaaaagga taacgcctat tatacagagc aacccattga tctggtgccc
     3901 acccagccac tgcctaacgc ttcttttgat aacttcaagt taacctgtag taatacaaaa
     3961 tttgccgacg acctgaacca gatgaccggc tttaccaagc ccgctagtag agagctgagc
     4021 gtgaccttct tccctgatct gaacggcgat gtggtggcaa tcgattacag acattatagc
     4081 gcctccttta agaaaggcgc taaactgctg cacaagccta tcgtgtggca cattaatcag
     4141 gccaccacca aaacaacctt caaaccaaat acatggtgcc tgaggtgcct gtggtctacc
     4201 aagcccgtgg ataccagcaa ctcctttgaa gtgctggctg tggaggacac acagggcatg
     4261 gacaacctgg cctgcgagag ccagcagcct acctccgagg aggtggtgga gaaccccacc
     4321 atccagaagg aggtaatcga gtgcgacgtg aaaaccaccg aggtggtggg caacgtgatc
     4381 ctgaagccct ccgacgaggg cgtgaaggtg acccaggagc tgggccacga ggacctgatg
     4441 gccgcttacg tggagaacac cagcatcact atcaagaagc ctaacgagct gtccctggct
     4501 ctggggctca agaccatcgc cacacacggc attgccgcca tcaacagcgt gccctggagc
     4561 aagatcctgg cctacgtgaa gcccttcctg ggccaggccg ccatcaccac cagcaattgc
     4621 gccaagcgcc tggcccagag ggtgttcaat aactacatgc cctacgtgtt taccctgctg
     4681 ttccagctgt gtaccttcac caagtccacc aatagccgca tcagagccag cctgcccacc
     4741 accatcgcca agaactcggt gaagtcagtg gccaagctgt gcctggacgc cggcatcaat
     4801 tacgtgaaaa gccctaagtt ctccaagctg ttcaccatcg ccatgtggct gctgctgctg
     4861 tccatctgcc tgggcagcct gatctgcgtg accgccgcct tcggcgtgct gctgagcaac
     4921 ttcggcgccc ctagctactg taatggcgtg cgggagctgt acctgaacag cagcaacgtg
     4981 accacaatgg atttctgcga gggctccttc ccctgctcca tctgtctgag cggcctggac
     5041 agcctggaca gctaccccgc cctggagacc atccaggtga ccatctccag ctacaagctg
     5101 gatctgacca tcctgggcct ggccgccgag tgggtgctgg cttatatgct gttcacaaag
     5161 ttcttctacc tgctgggcct gagcgccatc atgcaggtgt tcttcggcta cttcgccagc
     5221 cacttcatca gcaacagctg gctgatgtgg ttcatcatca gcatcgtgca gatggccccc
     5281 gtgagcgcta tggtgagaat gtacatcttc ttcgccagct tctactacat ctggaagtcc
     5341 tacgtgcaca tcatggatgg ctgcaccagc agcacctgca tgatgtgcta caagaggaat
     5401 cgcgccacca gagtggagtg caccaccatc gtgaacggca tgaagcggag cttttacgtg
     5461 tacgctaacg gcggccgggg cttctgcaag acccacaact ggaactgcct gaactgcgac
     5521 accttctgca ccggctccac cttcatctct gacgaggtgg cccgcgacct gagcctgcag
     5581 ttcaagaggc ccatcaaccc caccgaccag agcagctaca tcgtggactc cgtggccgtg
     5641 aagaacggag ccctgcacct gtacttcgac aaggccggcc agaaaaccta cgagcggcac
     5701 cccctgagcc acttcgtgaa cctggacaac ctgagagcca acaataccaa gggcagcctg
     5761 cccatcaacg tgatcgtgtt cgacggcaag tccaagtgcg atgagtccgc ctccaagagc
     5821 gccagcgtgt actactccca gctgatgtgc cagcctatcc tgctgctgga ccaggccctg
     5881 gtgtccgacg tgggagatag caccgaagtg tctgtgaaga tgtttgacgc ttacgtggac
     5941 accttctctg ctacattcag cgtgcctatg gagaaactga aggccctcgt cgccaccgcc
     6001 cacagcgagc tggccaaggg agtggctctg gacggcgtac tgtccacatt tgtgagcgcc
     6061 gccaggcagg gcgtggtgga taccgacgtt gataccaaag atgtgattga gtgtctgaag
     6121 ctgagccatc atagcgatct ggaggtgacc ggagactctt gcaacaactt tatgctgact
     6181 tataataagg tggagaatat gacaccaagg gacctgggcg cctgcatcga ctgtaatgcc
     6241 agacacatca atgcccaggt tgccaagtca cataacgtgt ccctgatttg gaatgtgaag
     6301 gattacatga gcctctctga acagctgaga aagcagatta gaagcgccgc caagaagaat
     6361 aacattccct ttagactgac ctgcgccacc accagacagg tggtgaatgt gatcacaacc
     6421 aagattagcc tgaagggagg cggcaaaccc attcctaatc ctctgctggg actggatagc
     6481 acctgaacgc gtcggatcct gagaacttca gggtgagttt ggggaccctt gattgttctt
     6541 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     6601 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     6661 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     6721 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     6781 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     6841 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     6901 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacaccctgg
     6961 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     7021 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctctttcc
     7081 tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc aaagaattca
     7141 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     7201 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     7261 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     7321 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     7381 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     7441 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     7501 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     7561 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     7621 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgag gagctttttg
     7681 caaaagccta ggcctccaaa aaagcctctt cactacttct ggaatagctc agaggccgag
     7741 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     7801 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     7861 taattgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7921 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7981 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8521 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8701 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8761 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8821 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8881 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8941 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9001 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9061 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9121 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9181 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9241 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9301 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9361 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9421 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9481 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9541 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9601 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9661 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9721 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9781 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9841 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     9901 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9961 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    10021 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    10081 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10141 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
    10201 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
    10261 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
    10321 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
    10381 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
    10441 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
    10501 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
    10561 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
    10621 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10681 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10741 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
    10801 attcgagctc gccc
//
