LOCUS       sequence-353688-       11245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1102
FEATURES             Location/Qualifiers
     source          1..11245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4183..4771
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4236..4256)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4654..4665)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4843..5076
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5154..5275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5191..5210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5245..5264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5315..5450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5470..5489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5471..5489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5499..5516)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5499..5515)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5508..5530)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5657..6112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5744..5763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5954..5975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6138..6242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6243..6311,6312..7103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6461..6480)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7274..7862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7763..7782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8016..8033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8150..8171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8186..8191,8192..8209,8210..8216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8224..8240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8229..8251
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8248..8264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8248..8264
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8283..8303
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8285..8303
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8331..8557
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8558..8738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8785..8910
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9403..9636
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9821..9865
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10096..10306
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10118..10314
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10165..10300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10346..10948
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10346..10365)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10842..10862
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10991..11108
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 cttgctgggt aagcgctgtg acgtccccac caacggctgc ggacccgacc gctggaactc
     1261 cgcgttcacc cgcaaagacg agatcatcac cagcctcgtg tctgccttag actccatgtg
     1321 ctcagcgctg tccaaactga acgccgaggt ggcctgtgtc gccgtgcacg atgagagcgc
     1381 ctttgtggtg ggcacagaga aggggagaat gttcctgaat gcccggaagg agctacagtc
     1441 agacttcctc aggttctgcc gagggccccc gtggaaggat ccggaggcag agcaccccaa
     1501 gaaggtgcag cggggcgagg gtggaggccg tagcctccct cggtcctccc tggaacatgg
     1561 ctcagatgtg taccttctgc ggaagatggt agaggaggtg tttgatgttc tttatagcga
     1621 ggccctggga agggccagtg tggtgccact gccctatgag aggctgctca gggagccagg
     1681 gctgctggcc gtgcaggggc tgcccgaagg cctggccttc cgaaggccag ccgagtatga
     1741 ccccaaggcc ctcatggcca tcctggaaca cagccaccgc atccgcttca agctcaagag
     1801 gccacttgag gatggcgggc gggactcgaa ggccctggtg gagctgaacg gtgtctccct
     1861 gattcccaag gggtcacggg actgtggcct gcatggccag gcccccaagg tgccacccca
     1921 ggacctgccc ccaaccgcca cctcctcctc catggccagc ttcctgtaca gcacggcgct
     1981 ccccaaccac gccatccgag agctcaagca ggaagcacct tcctgccccc ttgcccccag
     2041 cgacctgggc ctgagtcggc ccatgccaga gcccaaggcc accggtgccc aagacttctc
     2101 cgactgttgt ggacagaagc ccactgggcc tggtgggcct ctcatccaga acgtccatgc
     2161 ctccaagcgc attctcttct ccatcgtcca tgacaagtca gagaagtggg acgccttcat
     2221 aaaggaaacc gaggacatca acacgctccg ggagtgtgtg cagatcctgt ttaacagcag
     2281 atatgcggaa gccctgggcc tggaccacat ggtccccgtg ccctaccgga agattgcctg
     2341 tgacccggag gctgtggaga tcgtgggcat cccggacaag atccccttca agcgcccctg
     2401 cacttatgga gtccccaagc tgaagcggat cctggaggag cgccatagta tccacttcat
     2461 cattaagagg atgtttgatg agcgaatttt cacagggaac aagtttacca aagacaccac
     2521 gaagctggag ccagccagcc cgccagagga cacctctgca gaggtctcta gggccaccgt
     2581 ccttgacctt gctgggaatg ctcggtcaga caagggcagc atgtctgaag actgtgggcc
     2641 aggaacctcc ggggagctgg gcgggctgag gccgatcaaa attgagccag aggatctgga
     2701 catcattcag gtcaccgtcc cagacccctc gccaacctct gaggaaatga cagactcgat
     2761 gcctgggcac ctgccatcgg aggattctgg ttatgggatg gagatgctga cagacaaagg
     2821 tctgagtgag gacgcgcggc ccgaggagag gcccgtggag gacagccacg gtgacgtgat
     2881 ccggcccctg cggaagcagg tggagctgct cttcaacaca cgatacgcca aggccattgg
     2941 catctcggag cccgtcaagg tgccgtactc caagtttctg atgcacccgg aggagctgtt
     3001 tgtggtggga ctgcctgaag gcatctccct ccgcaggccc aactgcttcg ggatcgccaa
     3061 gctccggaag attctggagg ccagcaacag catccagttt gtcatcaaga ggcccgagct
     3121 gctcactgag ggagtcaaag agcccatcat ggatagtcaa ggaactgcct cctcacttgg
     3181 cttctctccc cctgccctgc ccccagagag ggattccggg gaccctctgg tggacgagag
     3241 cctgaagaga cagggctttc aagaaaatta tgacgcgagg ctctcacgga tcgacatcgc
     3301 caacacacta agggagcagg tccaggacct tttcaataag aaatacgggg aagccttggg
     3361 catcaagtac ccggtccagg tcccctacaa gcggatcaag agtaaccccg gctccgtgat
     3421 catcgagggg ctgcccccag gaatcccgtt ccgaaagccc tgtaccttcg gctcccagaa
     3481 cctggagagg attcttgctg tggctgacaa gatcaagttc acagtcacca ggcctttcca
     3541 aggactcatc ccaaagcctg atgaagatga cgccaacaga ctcggggaga aggtgatcct
     3601 gcgggagcag gtgaaggaac tcttcaacga gaaatacggt gaggccctgg gcctgaaccg
     3661 gccggtgctg gtcccttata aactaatccg ggacagccca gacgccgtgg aggtcacggg
     3721 tctgcctgat gacatcccct tccggaaccc caacacgtac gacatccacc ggctggagaa
     3781 gatcctgaag gcccgagagc atgtccgcat ggtcatcatt aaccagctcc aaccctttgc
     3841 agaaatctgc aatgatgcca aggtgccagc caaagacagc agcattccca agcgcaagag
     3901 aaagcgggtc tcggaaggaa attccgtctc ctcttcctcc tcgtcttcct cttcctcgtc
     3961 ctctaacccg gattcagtgg catcggccaa ccagatctca ctcgtgcaat ggccaatgta
     4021 catggtggac tatgccggcc tgaacgtgca gctcccggga cctcttaatt actagtaatg
     4081 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4141 cgagctggtg gagaacttct catcgtaacg cgttaagtcg acaatcaacc tctggattac
     4201 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4261 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4321 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4381 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4441 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4501 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4561 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4621 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4681 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4741 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     4801 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4861 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4921 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4981 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5041 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     5101 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5161 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5221 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5281 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5341 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5401 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5461 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5521 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5581 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5641 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5701 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5761 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5821 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5881 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5941 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6001 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6061 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6181 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6241 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6301 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6361 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6421 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6481 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6541 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6601 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6661 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6721 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6781 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6841 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6901 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6961 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7021 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7081 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7141 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7201 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7381 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7921 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7981 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8041 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8101 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8161 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8221 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8281 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     8341 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     8401 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     8461 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     8521 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     8581 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8641 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8701 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8761 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8821 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8881 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8941 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9001 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9061 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9121 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9181 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9241 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9301 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9361 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9421 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9481 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9541 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9601 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9661 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9721 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9781 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9841 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9901 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9961 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10021 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
    10081 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
    10141 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10201 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10261 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
    10321 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
    10381 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    10441 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
    10501 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    10561 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    10621 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    10681 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    10741 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    10801 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    10861 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    10921 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    10981 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11041 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11101 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    11161 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    11221 ctaggtcttg aaaggagtgg gaatt
//
