LOCUS       sequence-353690-       11563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2892
FEATURES             Location/Qualifiers
     source          1..11563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4501..5089
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4554..4574)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4972..4983)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5161..5394
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5472..5593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5509..5528)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5563..5582
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5633..5768
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5788..5807)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5789..5807)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5817..5834)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5817..5833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5826..5848)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5975..6430
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6062..6081)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6272..6293
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6456..6560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6561..6629,6630..7421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6779..6798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7592..8180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8081..8100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8334..8351
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8468..8489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8504..8509,8510..8527,8528..8534)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8542..8558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8547..8569
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8566..8582
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8566..8582
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8601..8621
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8603..8621
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8649..8875
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8876..9056
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9103..9228
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9721..9954
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10139..10183
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10414..10624
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10436..10632
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10483..10618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10664..11266
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10664..10683)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11160..11180
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11309..11426
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 caactcgatg accccaccac caatcagtag ctatggcgag ccctgctgtc tccggcccct
     1261 ccccagtcag ggggccccca gtgtggggac agaagtcaag ttgaccaaga agcgggcact
     1321 gtccatctca cctctgtcgg atgccagcct ggacctgcag acggttatcc gcacctcacc
     1381 cagctccctc gtagctttca tcaactcgcg atgcacatct ccaggaggct cctacggtca
     1441 tctctccatt ggcaccatga gcccatctct gggattccca gcccagatga atcaccaaaa
     1501 agggccctcg ccttcctttg gggtccagcc ttgtggtccc catgactctg cccggggtgg
     1561 gatgatccca catcctcagt cccggggacc cttcccaact tgccagctga agtctgagct
     1621 ggacatgctg gttggcaagt gccgggagga acccttggaa ggtgatatgt ccagccccaa
     1681 ctccacaggc atacaggatc ccctgttggg gatgctggat gggcgggagg acctcgagag
     1741 agaggagaag cgtgagcctg aatctgtgta tgaaactgac tgccgttggg atggctgcag
     1801 ccaggaattt gactcccaag agcagctggt gcaccacatc aacagcgagc acatccacgg
     1861 ggagcggaag gagttcgtgt gccactgggg gggctgctcc agggagctga ggcccttcaa
     1921 agcccagtac atgctggtgg ttcacatgcg cagacacact ggcgagaagc cacacaagtg
     1981 cacgtttgaa gggtgccgga agtcatactc acgcctcgaa aacctgaaga cgcacctgcg
     2041 gtcacacacg ggtgagaagc catacatgtg tgagcacgag ggctgcagta aagccttcag
     2101 caatgccagt gaccgagcca agcaccagaa tcggacccat tccaatgaga agccgtatgt
     2161 atgtaagctc cctggctgca ccaaacgcta tacagatcct agctcgctgc gaaaacatgt
     2221 caagacagtg catggtcctg acgcccatgt gaccaaacgg caccgtgggg atggccccct
     2281 gcctcgggca ccatccattt ctacagtgga gcccaagagg gagcgggaag gaggtcccat
     2341 cagggaggaa agcagactga ctgtgccaga gggtgccatg aagccacagc caagccctgg
     2401 ggcccagtca tcctgcagca gtgaccactc cccggcaggg agtgcagcca atacagacag
     2461 tggtgtggaa atgactggca atgcaggggg cagcactgaa gacctctcca gcttggacga
     2521 gggaccttgc attgctggca ctggtctgtc cactcttcgc cgccttgaga acctcaggct
     2581 ggaccagcta catcaactcc ggccaatagg gacccggggt ctcaaactgc ccagcttgtc
     2641 ccacaccggt accactgtgt cccgccgcgt gggcccccca gtctctcttg aacgccgcag
     2701 cagcagctcc agcagcatca gctctgccta tactgtcagc cgccgctcct ccctggcctc
     2761 tcctttcccc cctggctccc caccagagaa tggagcatcc tccctgcctg gccttatgcc
     2821 tgcccagcac tacctgcttc gggcaagata tgcttcagcc agagggggtg gtacttcgcc
     2881 cactgcagca tccagcctgg atcggatagg tggtcttccc atgcctcctt ggagaagccg
     2941 agccgagtat ccaggataca accccaatgc aggggtcacc cggagggcca gtgacccagc
     3001 ccaggctgct gaccgtcctg ctccagctag agtccagagg ttcaagagcc tgggctgtgt
     3061 ccatacccca cccactgtgg cagggggagg acagaacttt gatccttacc tcccaacctc
     3121 tgtctactca ccacagcccc ccagcatcac tgagaatgct gccatggatg ctagagggct
     3181 acaggaagag ccagaagttg ggacctccat ggtgggcagt ggtctgaacc cctatatgga
     3241 cttcccacct actgatactc tgggatatgg gggacctgaa ggggcagcag ctgagcctta
     3301 tggagcgagg ggtccaggct ctctgcctct tgggcctggt ccacccacca actatggccc
     3361 caacccctgt ccccagcagg cctcatatcc tgaccccacc caagaaacat ggggtgagtt
     3421 cccttcccac tctgggctgt acccaggccc caaggctcta ggtggaacct acagccagtg
     3481 tcctcgactt gaacattatg gacaagtgca agtcaagcca gaacaggggt gcccagtggg
     3541 gtctgactcc acaggactgg caccctgcct caatgcccac cccagtgagg ggcccccaca
     3601 tccacagcct ctcttttccc attaccccca gccctctcct ccccaatatc tccagtcagg
     3661 cccctatacc cagccacccc ctgattatct tccttcagaa cccaggcctt gcctggactt
     3721 tgattccccc acccattcca cagggcagct caaggctcag cttgtgtgta attatgttca
     3781 atctcaacag gagctactgt gggagggtgg gggcagggaa gatgcccccg cccaggaacc
     3841 ttcctaccag agtcccaagt ttctgggggg ttcccaggtt agcccaagcc gtgctaaagc
     3901 tccagtgaac acatatggac ctggctttgg acccaacttg cccaatcaca agtcaggttc
     3961 ctatcccacc ccttcaccat gccatgaaaa ttttgtagtg ggggcaaata gggcttcaca
     4021 tagggcagca gcaccacctc gacttctgcc cccattgccc acttgctatg ggcctctcaa
     4081 agtgggaggc acaaacccca gctgtggtca tcctgaggtg ggcaggctag gagggggtcc
     4141 tgccttgtac cctcctcccg aaggacaggt atgtaacccc ctggactctc ttgatcttga
     4201 caacactcag ctggactttg tggctattct ggatgagccc caggggctga gtcctcctcc
     4261 ttcccatgat cagcggggca gctctggaca taccccacct ccctctgggc cccccaacat
     4321 ggctgtgggc aacatgagtg tcttactgag atccctacct ggggaaacag aattcctcaa
     4381 ctctagtgcc tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     4441 tatatcttgt ggaaaggacg agcccactct gtgagcgttt atgggacgcg ttaagtcgac
     4501 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4561 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4621 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4681 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4741 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4801 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4861 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4921 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4981 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5041 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     5101 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5161 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5221 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5281 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5341 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     5401 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5461 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5521 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5581 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     5641 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5701 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5761 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     5821 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5881 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5941 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6421 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6781 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6901 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6961 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7021 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7081 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7141 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7201 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7261 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7321 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7381 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7441 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7501 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7921 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8161 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8221 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8281 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8341 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8401 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8461 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     8521 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     8581 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     8641 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     8701 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     8761 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     8821 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     8881 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8941 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9001 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     9061 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     9121 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     9181 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     9241 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     9301 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     9361 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     9421 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     9481 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     9541 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     9601 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9661 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9721 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9781 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     9841 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     9901 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     9961 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    10021 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    10081 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    10141 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    10201 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    10261 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    10321 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
    10381 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
    10441 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    10501 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10561 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    10621 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
    10681 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    10741 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
    10801 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    10861 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    10921 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    10981 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    11041 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    11101 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    11161 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    11221 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
    11281 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    11341 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    11401 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    11461 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
    11521 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
