LOCUS       sequence-353691-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, 
            R1379A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Su Y, Iacob RE, Li J, Engen JR, Springer TA
  TITLE     Dynamics of integrin alpha5beta1, fibronectin, and their complex 
            reveal sites of interaction and conformational change.
  JOURNAL   J Biol Chem. 2022 Sep;298(9):102323. doi: 10.1016/j.jbc.2022.102323.
            Epub 2022 Aug 2.
  PUBMED    35931112
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-09"; volume: "298(9):102323. doi"; pages: " 
            10.1016/j.jbc.2022.102323. Epub 2022 Aug 2"
COMMENT     Sequence Label: FnRPR8-10
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..30
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgcaccacc accaccatca tcaccatcac gaaaacttat acttccaggg tgttcctcct
       61 cccactgacc tgcgattcac caacattggt ccagacacca tgcgtgtcac ctgggctcca
      121 cccccatcca ttgatttaac caacttcctg gtgcgttact cacctgtgaa aaatgaggaa
      181 gatgttgcag agttgtcaat ttctccttca gacaatgcag tggtcttaac aaatctcctg
      241 cctggtacag aatatgtagt gagtgtctcc agtgtctacg aacaacatga gagcacacct
      301 cttagaggaa gacagaaaac aggtcttgat tccccaactg gcattgactt ttctgatatt
      361 actgccaact cttttactgt gcactggatt gctcctcgag ccaccatcac tggctacagg
      421 atccgccatc atcccgagca cttcagtggg agacctcgag aagatgcggt ggcgcactct
      481 gcgaattcca tcaccctcac caacctcact ccaggcacag agtatgtggt cagcatcgtt
      541 gctcttaatg gcagagagga aagtccctta ttgattggcc aacaatcaac agtttctgat
      601 gttccgaggg acctggaagt tgttgctgcg acccccacca gcctactgat cagctgggat
      661 gctcctgctg tcacagtgag atattacagg atcacttacg gagagacagg aggaaatagc
      721 cctgtccagg agttcactgt gcctgggagc aagtctacag ctaccatcag cggccttaaa
      781 cctggagttg attataccat cactgtgtat gctgtcactg gccgtggaga cagccccgca
      841 agcagcaagc caatttccat taattaccga acatgatag
//
