LOCUS       sequence-353692-       10513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2895
FEATURES             Location/Qualifiers
     source          1..10513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3451..4039
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3504..3524)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3922..3933)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4111..4344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4422..4543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4459..4478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4513..4532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4583..4718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4738..4757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4739..4757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4767..4784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4767..4783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4776..4798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4925..5380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5012..5031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5222..5243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5406..5510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5511..5579,5580..6371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5729..5748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6542..7130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7031..7050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7284..7301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7418..7439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7454..7459,7460..7477,7478..7484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7492..7508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7497..7519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7516..7532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7516..7532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7551..7571
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7553..7571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7599..7825
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7826..8006
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8053..8178
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8671..8904
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9089..9133
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9364..9574
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9386..9582
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9433..9568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9614..10216
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9614..9633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10110..10130
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10259..10376
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 cgccaacatg tacagggtcg gagactacgt ctactttgag aactcctcca gcaacccata
     1261 cctgatccgg agaatcgagg agctcaacaa gacggccaat gggaacgtgg aggccaaagt
     1321 ggtgtgcttc taccggaggc gggacatctc cagcaccctc atcgccctgg ccgacaagca
     1381 cgcaaccctg tcagtctgct ataaggccgg accgggggcg gacaacggcg aggaagggga
     1441 aatagaagag gaaatggaga acccggaaat ggtggacctg cccgagaaac taaagcacca
     1501 gctgcggcat cgggagctgt tcctctcccg gcagctggag tctctgcccg ccacgcacat
     1561 caggggcaag tgcagcgtca ccctgctcaa cgagaccgag tcgctcaagt cctacctgga
     1621 gcgggaggat ttcttcttct attctctagt ctacgaccca cagcagaaga ccctgctggc
     1681 agataaagga gagattcgag taggaaaccg gtaccaggca gacatcaccg acttgttaaa
     1741 agaaggcgag gaggatggcc gagaccagtc caggttggag acccaggtgt gggaggcgca
     1801 caacccactc acagacaagc agatcgacca gttcctggtg gtggcccgct ctgtgggcac
     1861 cttcgcacgg gccctggact gcagcagctc cgtccgacag cccagcctgc acatgagcgc
     1921 cgcagctgcc tcccgagaca tcaccctgtt ccacgccatg gatactctcc acaagaacat
     1981 ctacgacatc tccaaggcca tctcggcgct ggtgccgcag ggcgggcccg tgctctgcag
     2041 ggacgagatg gaggagtggt ctgcatcaga ggccaacctt ttcgaggaag ccctggaaaa
     2101 atatgggaag gatttcacgg acattcagca agattttctc ccgtggaagt cgctgaccag
     2161 catcattgag tactactaca tgtggaagac caccgacaga tacgtgcagc agaaacgctt
     2221 gaaagcagct gaagctgaga gcaagttaaa gcaagtttat attcccaact ataacaagcc
     2281 aaatccgaac caaatcagcg tcaacaacgt caaggccggt gtggtgaacg gcacgggggc
     2341 gccgggccag agccctgggg ctggccgggc ctgcgagagc tgttacacca cacagtctta
     2401 ccagtggtat tcttggggtc cccctaacat gcagtgtcgt ctctgcgcat cttgttggac
     2461 atattggaag aaatatggtg gcttgaaaat gccaacccgg ttagatggag agaggccagg
     2521 accaaaccgc agtaacatga gtccccacgg cctcccagcc cggagcagcg ggagccccaa
     2581 gtttgccatg aagaccaggc aggctttcta tctgcacacg acgaagctga cgcggatcgc
     2641 ccggcgcctg tgccgtgaga tcctgcgccc gtggcacgct gcgcggcacc cctacctgcc
     2701 catcaacagc gcggccatca aggccgagtg cacggcgcgg ctgcccgaag cctcccagag
     2761 cccgctggtg ctgaagcagg cggtacgcaa gccgctggaa gccgtgcttc ggtatcttga
     2821 gacccacccc cgccccccca agcctgaccc cgtgaaaagc gtgtccagcg tgctcagcag
     2881 cctgacgccc gccaaggtgg cccccgtcat caacaacggc tcccccacca tcctgggcaa
     2941 gcgcagctac gagcagcaca acggggtgga cggcaacatg aagaagcgcc tcttgatgcc
     3001 cagtaggggt ctggcaaacc acggacaggc caggcacatg ggaccaagcc ggaacctcct
     3061 gctcaacggg aagtcctacc ccaccaaagt gcgcctgatc cgggggggct ccctgccccc
     3121 agtcaagcgg cggcggatga actggatcga cgccccggat gacgtgttct acatggccac
     3181 agaggagacc aggaagatcc gcaagctgct ctcatcctcg gaaaccaagc gtgctgcccg
     3241 ccggccctac aagcccatcg ccctgcgcca gagccaggcc ctgccgccgc ggccaccgcc
     3301 acctgcgccc gtcaacgacg agcccatcgt catcgaggac tagtaatgaa ctagtccgta
     3361 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg acgatattcc
     3421 aactgcgaaa gtcgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     3481 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3541 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3601 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3661 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3721 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3781 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3841 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3901 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3961 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4021 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     4081 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4141 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4201 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4261 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4321 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     4381 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     4441 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4501 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4561 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4621 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4681 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4741 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4801 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4861 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4921 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5341 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5401 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5461 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5521 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5581 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5641 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5701 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5761 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5821 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5881 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5941 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6001 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6061 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7501 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     7561 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7621 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7681 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7741 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7801 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7861 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7921 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7981 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     8041 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     8101 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     8161 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     8221 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     8281 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8341 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8401 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8461 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8521 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8581 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8641 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8701 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8761 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8821 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8881 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8941 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     9001 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     9061 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     9121 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     9181 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     9241 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     9301 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9361 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     9421 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9481 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9541 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     9601 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     9661 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     9721 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     9781 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     9841 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9901 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9961 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
    10021 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
    10081 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
    10141 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
    10201 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
    10261 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    10321 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    10381 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
    10441 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
    10501 aggagtggga att
//
