LOCUS       sequence-353697-        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-2P under the 
            promoter hsp70 for Drosophila (insect) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, 
            Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, 
            Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, 
            Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias
            AS, Reimer J, Dieudonne S, St-Pierre F
  TITLE     Sustained deep-tissue voltage recording using a fast indicator 
            evolved for two-photon microscopy.
  JOURNAL   Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013.
  PUBMED    35985322
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013."
COMMENT     Sequence Label: pJFRC7-JEDI-2P
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3329..3423
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3457..3551
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3585..3679
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3713..3807
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3832..4070
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4017..4038
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          4085..4151
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     intron          5542..5607
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5737..5757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6029..6163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6066..6085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6120..6139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6205..6223)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6290..6394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6395..6463,6464..7255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6613..6632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7426..8014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7915..7934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    8101..8170
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(8395..8413)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(8865..8936,9338..9438,1..173,248..902,964..1279,
                     1483..1614,1685..2299)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
       61 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
      121 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
      181 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
      241 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
      301 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
      361 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
      421 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
      481 ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
      541 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
      601 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
      661 gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc
      721 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
      781 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
      841 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
      901 tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc
      961 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     1021 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     1081 ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga
     1141 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     1201 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     1261 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     1321 ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag
     1381 tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac
     1441 tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     1501 ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc
     1561 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     1621 catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat
     1681 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     1741 ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc
     1801 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     1861 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     1921 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     1981 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     2041 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     2101 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     2161 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
     2221 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     2281 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac
     2341 cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt
     2401 gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc
     2461 acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat
     2521 caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata catacttttc
     2581 aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc
     2641 gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac
     2701 tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc
     2761 aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata
     2821 tatttccctc acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt
     2881 tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg
     2941 ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc
     3001 aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg gatttcactg
     3061 gaactagggc gcgcctccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     3121 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     3181 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     3241 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     3301 agggccgcaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc
     3361 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     3421 gagcggagac tctagcccta gggcatgcct gcaggtcgga gtactgtcct ccgagcggag
     3481 tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     3541 tgtcctccga gcggagactc tagcgctagc gcatgcctgc aggtcggagt actgtcctcc
     3601 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     3661 cggagtactg tcctccgagc ggagactcta gcactagtgc atgcctgcag gtcggagtac
     3721 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     3781 cctccgagcg gagtactgtc ctccgagcgg agactctagc gacgtcgagc gccggagtat
     3841 aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt
     3901 cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt
     3961 aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa
     4021 ctactgaaat ctgccaagaa gtaattattg aatacaagaa gagaactctg aatagatcta
     4081 aaaggtaggt tcaaccactg atgcctaggc acaccgaaac gactaaccct aattcttatc
     4141 ctttacttca ggcggccgcg gctcgagcaa aatggagacg actgtgaggt atgaacaggg
     4201 gtcagagctc actaaaactt cgagctctcc aacagcagat gagcccacga taaagattga
     4261 tgatggtcgt gatgagggta atgaacaaga cagctgttcc aataccatta ggagaaaaat
     4321 ttccccgttt gtgatgtcat ttggattcag agtatttgga gttgtgctta tcattgtaga
     4381 catcatagtg gtgattgtgg atctggccat cagtgagaag aaaagaggca ttagagagat
     4441 tcttgaaggt gtttccctgg ctatagcact cttcttcctt gttgatgttc tcatgagagt
     4501 gtttgttgaa ggcttcaaga actatttccg gtccaaactg gttactttgg atgcagtcat
     4561 agtagtgggc actctgctaa ttaatatgac ctactccttc tctgaccttg ctgcctttaa
     4621 cagcgagaac gtgtatatta ccgcggataa acagaaaaac ggcattaaag cgaactttac
     4681 cgtgcgccat aacgtggaag atggcagcgt gcagctggcg gatcattatc agcagaacac
     4741 cccgattggc gatggcccgg tgctgctgcc ggataaccat tatctgagcc accagaccgt
     4801 gctgagcaaa gatccgaacg aaaaacgcga tcacatggtg ctgctggaat ttgtgaccgc
     4861 agcgggcatt acacacggca tggatgaact gtatggcggc accggcggca gcgcgagcca
     4921 gggcgaagaa ctgtttaccg gcgtggtgcc gattctggtg gaactggatg gcgatgtgaa
     4981 cggccataaa tttagcgtgc gcggcgaagg cgaaggcgat gcgaccattg gcaaactgac
     5041 cctgaaattt atttgcacca ccggcaaact accggtgccg tggccgaccc tggtgaccac
     5101 cttaacctat ggcgtgcagt gctttagccg ctatccggat catatgaaac gccatgattt
     5161 ttttaaaagc gcgatgccgg aaggctatgt gcaggaacgc accattagct ttaaagatga
     5221 tggcaaatat aaaacccgcg cggtggtgaa atttgaaggc gataccctgg tgaaccgcat
     5281 tgaactgaaa ggcaccgatt ttaaagaaga tggcaacatt ctggggcata aactggaata
     5341 tgatacagat cagatgccgc acatggttac tcttcttcga gttctgaaaa ttgttatctt
     5401 aataagaata tttcgcctgg cttcacagaa gaaacaactt gaagtggtaa cctaatgatc
     5461 tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     5521 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     5581 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     5641 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     5701 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     5761 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     5821 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     5881 aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     5941 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6001 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     6061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6121 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgatctgg ccggccgttt
     6181 aaacgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6241 tgataataat ggtttcttag actcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6301 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6361 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6421 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6481 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6541 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6601 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6661 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6721 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6781 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6841 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6901 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6961 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7021 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7081 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7141 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7201 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7261 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7321 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7381 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7441 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7501 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7561 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7621 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7681 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7741 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7801 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7861 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7921 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7981 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8041 gttcctggcc ttttgctggc cttttgctca catgttaccg tcgacgatgt aggtcacggt
     8101 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
     8161 ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa
     8221 cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag
     8281 cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc
     8341 gacggtcacg gcgggcatgt cgacaagccg aacatatggg cgcgcctagt atgtatgtaa
     8401 gttaataaaa cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca
     8461 ctggatatca ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga
     8521 tgtaccaggt tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct
     8581 gtttgcctcc ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat
     8641 ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg
     8701 tcagcggttt cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg
     8761 tgccaaaact cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt
     8821 ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc
     8881 aggagctatt aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga
     8941 gtagtacatg tgcatacatc ttaagttcac ttgatctata ggaactgcga ttgcaacatc
     9001 aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa ttgcacaaac
     9061 aaatagtgac acgaaacaga ttattctggt agctgttctc gctatataag acaatttttg
     9121 agatcatatc atgatcaaga catctaaagg cattcatttt cgactatatt cttttttaca
     9181 aaaaatataa caaccagata ttttaagctg atcctagatg cacaaaaaat aaataaaagt
     9241 ataaacctac ttcgtaggat acttcggggt actttttgtt cggggttaga tgagcataac
     9301 gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca
     9361 gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc ggccacccag
     9421 tccgccggag gactccgg
//
