LOCUS       sequence-353699-        6876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soma and AIS-localized genetically encoded voltage indicator (GEVI) 
            JEDI-2P in AAV production vector for expression in excitatory 
            glutamatergic neurons using the promoter CaMKII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, 
            Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, 
            Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, 
            Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias
            AS, Reimer J, Dieudonne S, St-Pierre F
  TITLE     Sustained deep-tissue voltage recording using a fast indicator 
            evolved for two-photon microscopy.
  JOURNAL   Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013.
  PUBMED    35985322
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013."
COMMENT     Sequence Label: pAAV-CaMKIIa-JEDI-2P-Kv-WPRE
FEATURES             Location/Qualifiers
     source          1..6876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      145..733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     634..653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   795..924
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        943..2231
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      2252..2261
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3797..4385
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3850..3870)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4268..4279)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4417..4893
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4616..4635)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4933..5073
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4933..5062
                     /label=AAV2 ITR
     rep_origin      5148..5603
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5235..5254)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5445..5466
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5620..5639)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5739..5761
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5799..5817)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5885..5989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5990..6058,6059..6850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6208..6227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
       61 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      121 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      181 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      241 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      301 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      361 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      421 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      481 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      541 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      601 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      661 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      721 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      781 ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc
      841 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
      901 ccaactccat cactaggggt tcctgcggcc gcacgcgttt aacattatgg ccttaggtca
      961 cttcatctcc atggggttct tcttctgatt ttctagaaaa tgagatgggg gtgcagagag
     1021 cttcctcagt gacctgccca gggtcacatc agaaatgtca gagctagaac ttgaactcag
     1081 attactaatc ttaaattcca tgccttgggg gcatgcaagt acgatataca gaaggagtga
     1141 actcattagg gcagatgacc aatgagttta ggaaagaaga gtccagggca gggtacatct
     1201 acaccacccg cccagccctg ggtgagtcca gccacgttca cctcattata gttgcctctc
     1261 tccagtccta ccttgacggg aagcacaagc agaaactggg acaggagccc caggagacca
     1321 aatcttcatg gtccctctgg gaggatgggt ggggagagct gtggcagagg cctcaggagg
     1381 ggccctgctg ctcagtggtg acagataggg gtgagaaagc agacagagtc attccgtcag
     1441 cattctgggt ctgtttggta cttcttctca cgctaaggtg gcggtgtgat atgcacaatg
     1501 gctaaaaagc agggagagct ggaaagaaac aaggacagag acagaggcca agtcaaccag
     1561 accaattccc agaggaagca aagaaaccat tacagagact acaaggggga agggaaggag
     1621 agatgaatta gcttcccctg taaaccttag aacccagctg ttgccagggc aacggggcaa
     1681 tacctgtctc ttcagaggag atgaagttgc cagggtaact acatcctgtc tttctcaagg
     1741 accatcccag aatgtggcac ccactagccg ttaccatagc aactgcctct ttgccccact
     1801 taatcccatc ccgtctgtta aaagggccct atagttggag gtgggggagg taggaagagc
     1861 gatgatcact tgtggactaa gtttgttcgc atccccttct ccaaccccct cagtacatca
     1921 ccctggggga acagggtcca cttgctcctg ggcccacaca gtcctgcagt attgtgtata
     1981 taaggccagg gcaaagagga gcaggtttta aagtgaaagg caggcaggtg ttggggaggc
     2041 agttaccggg gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc
     2101 tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg
     2161 tcagtcaagc cggttctccg tttgcactca ggagcacggg caggcgagtg gcccctagtt
     2221 ctgggggcag ctctagagcg gtaccggatc cgccaccatg gagacgactg tgaggtatga
     2281 acaggggtca gagctcacta aaacttcgag ctctccaaca gcagatgagc ccacgataaa
     2341 gattgatgat ggtcgtgatg agggtaatga acaagacagc tgttccaata ccattaggag
     2401 aaaaatttcc ccgtttgtga tgtcatttgg attcagagta tttggagttg tgcttatcat
     2461 tgtagacatc atagtggtga ttgtggatct ggccatcagt gagaagaaaa gaggcattag
     2521 agagattctt gaaggtgttt ccctggctat agcactcttc ttccttgttg atgttctcat
     2581 gagagtgttt gttgaaggct tcaagaacta tttccggtcc aaactggtta ctttggatgc
     2641 agtcatagta gtgggcactc tgctaattaa tatgacctac tccttctctg accttgctgc
     2701 ctttaacagc gagaacgtgt atattaccgc ggataaacag aaaaacggca ttaaagcgaa
     2761 ctttaccgtg cgccataacg tggaagatgg cagcgtgcag ctggcggatc attatcagca
     2821 gaacaccccg attggcgatg gcccggtgct gctgccggat aaccattatc tgagccacca
     2881 gaccgtgctg agcaaagatc cgaacgaaaa acgcgatcac atggtgctgc tggaatttgt
     2941 gaccgcagcg ggcattacac acggcatgga tgaactgtat ggcggcaccg gcggcagcgc
     3001 gagccagggc gaagaactgt ttaccggcgt ggtgccgatt ctggtggaac tggatggcga
     3061 tgtgaacggc cataaattta gcgtgcgcgg cgaaggcgaa ggcgatgcga ccattggcaa
     3121 actgaccctg aaatttattt gcaccaccgg caaactaccg gtgccgtggc cgaccctggt
     3181 gaccacctta acctatggcg tgcagtgctt tagccgctat ccggatcata tgaaacgcca
     3241 tgattttttt aaaagcgcga tgccggaagg ctatgtgcag gaacgcacca ttagctttaa
     3301 agatgatggc aaatataaaa cccgcgcggt ggtgaaattt gaaggcgata ccctggtgaa
     3361 ccgcattgaa ctgaaaggca ccgattttaa agaagatggc aacattctgg ggcataaact
     3421 ggaatatgat acagatcaga tgccgcacat ggttactctt cttcgagttc tgaaaattgt
     3481 tatcttaata agaatatttc gcctggcttc acagaagaaa caacttgaag tggtaaccgg
     3541 ttcttcaggt tcatctggat cttccacgcg tcagtcccag cccatcctca acaccaagga
     3601 gatggccccg cagagcaagc ctccagagga gctggagatg agcagcatgc ccagccccgt
     3661 ggcccctctg cccgcacgca cggagggcgt catcgacatg cggagcatgt ccagcattga
     3721 cagcttcatc agctgtgcca cggacttccc tgaagccacc agattctgat aagaattcga
     3781 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3841 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3901 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3961 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4021 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccggaacttt
     4081 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4141 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4201 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
     4261 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4321 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4381 cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
     4441 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     4501 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     4561 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     4621 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     4681 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     4741 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4801 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4861 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     4921 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4981 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     5041 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     5101 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     5161 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5221 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5281 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5341 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     5401 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5461 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     5521 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5581 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     5641 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5701 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5761 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5821 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5881 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5941 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6001 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6061 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6121 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6181 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6241 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6301 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6361 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6421 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6481 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6541 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6601 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6661 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6721 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6781 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6841 gcattggtaa ctgtcagacc aagtttactc atatat
//
