LOCUS       sequence-353706-       10822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1812
FEATURES             Location/Qualifiers
     source          1..10822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3185..3196
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3760..4348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3813..3833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4231..4242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4420..4653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4731..4852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4768..4787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4822..4841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4892..5027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5047..5066)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5048..5066)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5076..5093)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5076..5092)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5085..5107)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5234..5689
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5321..5340)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5531..5552
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5715..5819
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5820..5888,5889..6680)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6038..6057)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6851..7439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7340..7359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7593..7610
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7727..7748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7763..7768,7769..7786,7787..7793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7801..7817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7806..7828
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7825..7841
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7825..7841
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7860..7880
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7862..7880
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7908..8134
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8135..8315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8362..8487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8980..9213
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9398..9442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9673..9883
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9695..9891
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9742..9877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9923..10525
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9923..9942)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10419..10439
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10568..10685
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 tagtgaatca aacatccctt ctggaacaat acagagtagg aaaggtttgc agaataagag
     1261 tcagtttagg accattgcac caaaaattgt gcccaaagtc ctaacgtcca gaatgctgcc
     1321 atgtcattca ccatcacgct ctgatcaggt gaatctggga ccctccatca actccaagct
     1381 gctggggatg tccacccaga actatgccct gatgcaggtt gctggccagg aggggacatt
     1441 ttctcttgtt gctctgccac atgttgcctc agctcagcca attcagaaac ccagaatgtc
     1501 cctacctgaa aacctgaaac ttcctattcc tagatatcaa ccccctagaa atagcaaagc
     1561 atcaagaaag aaacccatcc tgatctttcc taagagtggc tgtagcaaag ctcctgccca
     1621 aacccaaatg tgtcctcaga tgtccccttc cccacctcac caccctgaac tcctgtacaa
     1681 acccagtcca tttgaggaag taccatcact agagcaagcc ccagccagca ttagcacagc
     1741 tgcgctgacc aatggaagtg accatgggga cttgagacca ccagtgacca acacccatgg
     1801 cagtctgaac cctcctgcta ccccagcatc atccacacca gaggagcctg ccaagcagga
     1861 cctcacagct ctttcaggga aagcacactt tgtaagcaag ataacatcta gtaaaccttc
     1921 tgctgttgcc agtgaaaaat ttaaagaaca agttgatctt gcaaaaacca tgaccaattt
     1981 atcaccaacc attcttggca atgcagttca gttgatctct tcagtcccca aagggaaact
     2041 gccaatccca ccctactcaa gaatgaagac aatggaggtt tacaaaatca aatcagatgc
     2101 taacattgca ggtttttctt taccaggacc taaggccgac tgtgataaga taccctccac
     2161 cacagaaggc tttaatgcag ccaccaaggt ggcaagcagg ctacctgttc cacaagtgtc
     2221 acagcagagt gcctgtgaaa gtgccttttg tccacccacc aaacttgatc ttaaccacaa
     2281 aacaaaactg aacagtggag cagcaaagag aaaaggaaga aaacggaagg taccagatga
     2341 aattttggca tttcagggaa aaaggaggaa atatatcatt aataagtgta gagatggtaa
     2401 agaaagagta aaaaatgatc cccaagaatt cagagaccaa aagctgggga ccctgaaaaa
     2461 ataccgtagc attatgccca aacctatcat ggtcataccc actttggcct ccctggcttc
     2521 tccaactaca ctacagtccc agatgcttgg gggcctagga caggatgttt tgttaaataa
     2581 ttcactcact cctaaatatc ttggctgtaa gcaagacaac agctcttccc ctaagcccag
     2641 ctccgtgttc agaaatggat tctctggcat taagaagcct tggcacagat gtcacgtctg
     2701 caaccaccac ttccagttca aacagcacct tcgagaccac atgaatacac acaccaacag
     2761 acgcccttac agttgtcgga tttgtcgcaa gtcctatgta cgtcctggca gcctgagcac
     2821 acacatgaaa cttcatcatg gtgagaaccg tctgaagaaa ctcatgtgtt gtgagttttg
     2881 tgcaaaagtg tttggccaca tccgagtcta ttttggccat ctgaaagaag tgcatagggt
     2941 tgtgatcagc actgagcctg cgcccagtga actgcagcca ggagacatac caaagaacag
     3001 agacatgagt gtgcgaggca tggagggatc attggagagg gaaaacaagt ccaacctgga
     3061 agaagacttc cttctaaacc aggcagacga agtcaaatta caaatcaaat gtggtcgttg
     3121 tcagattact gctcagtctt ttgcggaaat aaaatttcat ttacttgatg ttcatggaga
     3181 ggaaattgag ggcaggctac aagaagggac cttcccagga agcaagggga ctcaggaaga
     3241 gttggtgcag cacgctagcc ccgactggaa aaggcatcct gagagaggga agccggagaa
     3301 ggttcattcc tcctccgagg aatcacatgc atgtccaaga ctgaaaaggc agctccacct
     3361 tcatcagaat ggcgtggaaa tgctcatgga aaatgaagga ccccagtcag gaaccaacaa
     3421 gccaagggaa acctgccagg gccctgagtg tcctggcctc cacacgtttc tcttgtggtc
     3481 ccattcaggc tttaactgcc tgctttgtgc agagatgctg ggacggaaag aggacctcct
     3541 ccaccactgg aagcaccagc ataactgtga ggacccttcc aaactgtggg ctattttaaa
     3601 tacggtctcc aaccagggag tgatcgaact ttccagtgaa gctgagaaat agtaatgaac
     3661 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     3721 taatcactat tagaccactg agggacgcgt taagtcgaca atcaacctct ggattacaaa
     3781 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3841 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3901 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3961 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4021 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4081 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4141 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4201 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4261 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4321 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     4381 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4441 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4621 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     4681 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4861 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4921 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4981 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     5041 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     5101 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5161 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5221 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5281 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5341 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5401 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5461 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5521 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5581 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5641 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     5701 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5761 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5821 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5881 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5941 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6001 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6061 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6121 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6181 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6241 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6301 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6361 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6421 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6481 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6541 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6601 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6661 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6721 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6781 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6841 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6901 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6961 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7021 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7081 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7141 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7201 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7261 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7321 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7381 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7441 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7501 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7561 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7621 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7681 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7741 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7801 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     7861 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     7921 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7981 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     8041 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8101 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8161 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8221 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8281 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8341 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8401 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     8461 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     8521 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8581 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     8641 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     8701 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     8761 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     8821 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8881 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8941 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9001 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9061 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9121 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9181 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9241 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9301 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9361 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9421 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9481 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9541 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9601 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     9661 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     9721 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9781 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9841 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     9901 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9961 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    10021 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
    10081 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
    10141 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    10201 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    10261 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    10321 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    10381 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    10441 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    10501 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
    10561 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    10621 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    10681 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    10741 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    10801 ggtcttgaaa ggagtgggaa tt
//
