LOCUS       sequence-353716-       14182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2846
FEATURES             Location/Qualifiers
     source          1..14182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7120..7708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7173..7193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7591..7602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7780..8013
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8091..8212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8128..8147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8182..8201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8252..8387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8407..8426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8408..8426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8436..8453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8436..8452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8445..8467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8594..9049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8681..8700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8891..8912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9075..9179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9180..9248,9249..10040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9398..9417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10211..10799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10700..10719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10953..10970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11087..11108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11123..11128,11129..11146,11147..11153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11161..11177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11166..11188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11185..11201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11185..11201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11220..11240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11222..11240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11268..11494
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11495..11675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11722..11847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12340..12573
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12758..12802
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        13033..13243
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        13055..13251
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      13102..13237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13283..13885
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(13283..13302)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13779..13799
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13928..14045
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgca
     1201 gtcctttcgg gagcaaagca gttaccacgg aaaccagcaa agctacccac aggaggtaca
     1261 cggctcatcc cggctagaag agttcagccc tcgtcaggcc cagatgttcc agaattttgg
     1321 aggtacaggt ggcagtagtg gcagcagtgg cagtggcagt ggtggtggac gacgaggagc
     1381 agcagctgct gcggcagcga tggctagcga gacctctggc catcaaggtt accagggttt
     1441 caggaaagag gctggagatt tttactacat ggcaggcaac aaagaccccg tgactacagg
     1501 aaccccacag cctcctcagc gaaggccttc tgggcctgtg cagagctatg gaccccccca
     1561 ggggagcagc tttggcaatc agtatgggag tgagggtcat gtgggccagt ttcaagcaca
     1621 gcactctggc cttggcggtg tgtcacatta tcagcaggat tacactgggc ctttctctcc
     1681 agggagtgct cagtaccaac agcaggcttc cagccagcag cagcagcagc aagtccagca
     1741 gttgagacaa cagctttacc agtcccatca gcccctgcca caggccactg gccaaccagc
     1801 atccagctca tcccatctac agccaatgca gcggccctca actctgccat cctctgctgc
     1861 tggttaccag ttaagagtgg gtcagtttgg ccaacactat cagtcttctg cttcctcctc
     1921 ctcctcctcc tccttccctt caccacagcg ttttagccag tctggacaga gctatgatgg
     1981 cagttacaat gtgaatgctg gatctcagta tgaaggacac aatgtgggtt ctaatgcaca
     2041 ggcttatgga acacaatcca attacagcta tcagcctcaa tctatgaaga attttgaaca
     2101 ggcaaagatt ccacaaggga cccaacaggg gcagcagcag cagcaaccgc agcaacaaca
     2161 acacccttct cagcatgtga tgcagtatac taacgctgcc accaagctgc ccctgcaaag
     2221 ccaagtgggg cagtacaacc agcctgaggt tcctgtgagg tcccccatgc agtttcacca
     2281 gaacttcagc cccatttcta acccttctcc agctgcctct gtggttcagt ctccaagctg
     2341 tagttctacc ccatctcctc tcatgcagac tggggagaat ctccagtgtg ggcaaggcag
     2401 tgtgcctatg ggttccagaa acagaatttt acagttaatg cctcaactca gtccaacccc
     2461 atcaatgatg cccagtccta attctcatgc tgcaggcttc aaagggtttg gactagaagg
     2521 ggtaccagaa aagcgactga cagatcctgg gttgagtagt ttgagtgctc tgagtactca
     2581 agtggccaat cttcctaaca ctgtccagca catgttactt tctgatgccc tgactcctca
     2641 gaagaagacc tccaagaggc cctcatcttc caagaaagca gatagctgca caaattctga
     2701 aggctcctca caacctgaag aacagctgaa gtcccctatg gcagagtcat tagatggagg
     2761 ctgctccagc agttcagagg atcaaggcga gagagtgcgg caactaagtg gccagagcac
     2821 cagctctgac accacctaca agggtggagc ctctgagaaa gctggctcct caccggcaca
     2881 aggtgctcag aatgaacccc ccagactcaa tgctagtcct gccgcaagag aagaggccac
     2941 ctcaccaggc gctaaggaca tgccattgtc atccgacggg aacccaaagg ttaatgagaa
     3001 gactgttggg gtgattgtct cccgggaagc catgacaggt cgggtagaaa agcctggtgg
     3061 acaagataaa ggctcccaag aggatgatcc tgcagccact caaaggccac ctagcaatgg
     3121 tggggcaaag gaaaccagtc atgcatcact tccccagcca gagcctccag gaggaggagg
     3181 gagcaaagga aacaagaatg gcgataacaa ctccaaccat aatggagaag gaaatggcca
     3241 gagtggccac tctgcagcgg gccctggttt tacgagcaga actgagccta gcaaatctcc
     3301 tggaagtctg cgctatagtt acaaagatag tttcgggtca gccgtgccac gaaatgtcag
     3361 tggctttcct cagtatccta cagggcaaga aaagggagat ttcactggcc atggggaacg
     3421 aaagggtaga aatgaaaaat tcccaagcct cctgcaggaa gtgcttcagg gttaccacca
     3481 ccaccctgac aggagatatt ctaggagtac tcaagagcat caggggatgg ctggtagcct
     3541 agaaggaacc acaaggccca atgtcttggt tagtcaaacc aatgaattag ctagcagggg
     3601 ccttctgaac aaaagcattg ggtctctatt agaaaatccc cactggggcc cctgggaaag
     3661 gaaatcaagc agcacagctc ctgaaatgaa acagatcaat ttgactgact atccaattcc
     3721 cagaaagttt gaaatagagc ctcagtcatc agcacatgag cctgggggtt ccctctctga
     3781 aagaagatca gtgatctgtg atatttctcc actaagacag attgtcaggg acccaggggc
     3841 tcactcactg ggacacatga gtgccgacac cagaattggg aggaatgacc gtctcaatcc
     3901 aactttaagt cagtcggtca ttcttcctgg tggtttggtg tccatggaaa ccaagctgaa
     3961 atcccagagc gggcagataa aagaggaaga ctttgaacag tctaaatctc aagctagttt
     4021 caacaacaag aaatctggag accactgcca tcctcctagc atcaagcatg agtcttaccg
     4081 cggcaatgcc agccctggag cagcaaccca tgattccctt tcagactatg gcccgcaaga
     4141 cagcagaccc acgccaatgc ggcgggtccc tggcagagtt ggtggtcggg agggcatgag
     4201 gggtcggtcc ccttctcaat atcatgactt tgcagaaaaa ttgaaaatgt ctcctgggcg
     4261 gagcagaggc ccagggggag accctcatca catgaatcca cacatgacct tttcagagag
     4321 ggctaaccgg agttctttac acactccctt ttctcccaac tcagaaaccc tggcctctgc
     4381 ttatcatgca aatactcggg ctcatgctta tggggaccct aacgcaggtt tgaattctca
     4441 gctgcattat aagagacaga tgtaccaaca gcaaccagag gagtataaag actggagcag
     4501 cggttctgct cagggagtaa ttgctgcagc acagcacagg caggaggggc cacggaagag
     4561 tccaaggcag cagcagtttc ttgacagagt acggagccct ctgaaaaatg acaaagatgg
     4621 tatgatgtat ggcccaccag tggggactta ccatgacccc agtgcccagg aggctgggcg
     4681 ctgcctaatg tctagtgatg gtctgcctaa caagggcatg gaattaaagc atggctccca
     4741 gaagttacaa gaatcctgtt gggatctttc tcggcaaact tctccagcca aaagcagcgg
     4801 tcctccagga atgtccagtc aaaaaaggta tgggccgccc catgagactg atggacatgg
     4861 actagctgag gctacacagt catccaaacc tggtagtgtt atgctgagac ttccaggcca
     4921 ggaggatcat tcttctcaaa accccttaat catgaggagg cgtgttcgtt cttttatctc
     4981 tcccattccc agtaagagac agtcacaaga tgtaaagaac agtagcactg aagataaagg
     5041 tcgcctcctt cactcatcaa aagaaggcgc tgataaagca ttcaattcct atgcccatct
     5101 ttctcacagt caggatatca agtctatccc taagagagat tcctccaagg accttccaag
     5161 tccagatagt agaaactgcc ctgctgttac cctcacaagc cctgctaaga ccaaaatact
     5221 gcccccacgg aaaggacggg gattgaaatt ggaagctata gttcagaaga ttacatcccc
     5281 aaatattagg aggagcgcat cttcgaacag tgcggaggct gggggagaca cggttacgct
     5341 tgatgatata ctgtctttga agagtggtcc tcctgaaggt gggagtgttg ctgttcagga
     5401 tgctgacata gagaagagaa aaggtgaggt ggcttcggac ctagtcagtc cagcaaacca
     5461 ggagttgcac gtagagaaac ctcttccaag gtcttcagaa gagtggcgtg gcagcgtgga
     5521 tgacaaagtg aagacagaga cacatgcaga aacagttact gccggaaagg aaccccctgg
     5581 tgccatgaca tccacaacct cacagaagcc tggtagtaac caagggagac cagatggttc
     5641 cctgggtgga acagcacctt taatctttcc agactcaaag aatgtacctc cagtgggcat
     5701 attggcccct gaggcaaacc ccaaggctga agagaaggag aacgatacag tgacgatttc
     5761 accgaagcaa gagggtttcc ctccaaaggg atatttccca tcaggaaaga agaaggggag
     5821 acccattggt agtgtgaata agcaaaagaa acagcagcag ccaccgcctc caccccctca
     5881 gcccccacag ataccagaag gttctgcaga tggagagcca aagccaaaaa aacagaggca
     5941 aaggagggag agaaggaagc ctggggccca gccgaggaag cgaaaaacca aacaagcagt
     6001 tcccattgtg gaaccccaag aacctgagat caaactaaaa tatgccaccc agccactgga
     6061 taaaactgat gccaagaaca agtcttttta cccttacatc catgtagtaa ataagtgtga
     6121 acttggagcc gtttgtacaa tcatcaatgc tgaggaagaa gaacagacca aattagtgag
     6181 gggcaggaag ggtcagaggt cactgacccc tccacctagc agcactgaaa gcaaggcgct
     6241 cccggcctcg tcctttatgc tgcagggacc tgttgtgaca gagtcttcgg ttatggggca
     6301 cctggtttgc tgtctgtgtg gcaagtgggc cagttaccgg aacatgggtg acctctttgg
     6361 acctttttat ccccaagatt atgcagccac tctcccgaag aatccacctc ctaagagggc
     6421 cacagaaatg cagagcaaag ttaaggtacg gcacaaaagt gcttctaatg gctccaagac
     6481 ggacactgag gaggaggaag agcagcagca gcagcagaag gagcagagaa gcctggccgc
     6541 acaccccagg tttaagcggc gccaccgctc ggaagactgt ggtggaggcc ctcggtccct
     6601 gtccaggggg ctcccttgta aaaaagcagc cactgagggc agcagtgaaa agactgtttt
     6661 ggactcgaag ccctccgtgc ccaccacttc agaaggtggc cctgagctgg agttacaaat
     6721 ccctgaacta cctcttgaca gcaatgaatt ttgggtccat gagggttgta ttctctgggc
     6781 caatggaatc tacctggttt gtggcaggct ctatggcctg caggaagcgc tggaaatagc
     6841 cagagagatg aaatgttccc actgccagga ggcaggcgcc accttgggct gctacaacaa
     6901 aggctgctcc ttccgatacc attacccgtg tgccattgat gcagattgtt tgctacatga
     6961 ggagaacttc tcggtgaggt gccctaagca caaggtgaga ctgtggagat agtaatgaac
     7021 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     7081 ggcctcccgt aaacgataaa gaccacgcgt taagtcgaca atcaacctct ggattacaaa
     7141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7501 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7681 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     7741 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7801 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7861 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7921 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7981 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     8041 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8101 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8161 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8221 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8281 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8341 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     8401 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     8461 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8521 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8581 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8641 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8701 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8761 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8821 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8881 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8941 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9001 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     9061 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9121 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9181 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9241 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9301 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9361 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9421 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9481 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9541 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9601 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9661 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9721 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9781 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9841 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9901 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9961 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10021 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10081 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10141 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10201 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10261 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10321 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10381 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10441 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10501 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10561 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10621 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10681 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10741 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10801 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10861 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10921 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10981 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    11041 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11101 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11161 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11221 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
    11281 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
    11341 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
    11401 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
    11461 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
    11521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    11581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    11641 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    11701 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    11761 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    11821 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    11881 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    11941 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    12001 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    12061 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    12121 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    12181 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    12241 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    12301 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    12361 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    12421 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    12481 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    12541 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    12601 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    12661 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    12721 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    12781 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    12841 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    12901 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    12961 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
    13021 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
    13081 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    13141 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    13201 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
    13261 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    13321 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    13381 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
    13441 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
    13501 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    13561 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    13621 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    13681 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    13741 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    13801 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    13861 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
    13921 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    13981 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    14041 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    14101 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    14161 ggtcttgaaa ggagtgggaa tt
//
