LOCUS       sequence-353717-        7611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soma and AIS-localized genetically encoded voltage indicator (GEVI) 
            JEDI-2P expressed under strong mammalian promoter (CAG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, 
            Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, 
            Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, 
            Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias
            AS, Reimer J, Dieudonne S, St-Pierre F
  TITLE     Sustained deep-tissue voltage recording using a fast indicator 
            evolved for two-photon microscopy.
  JOURNAL   Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013.
  PUBMED    35985322
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013."
COMMENT     Sequence Label: pcDNA3.1/Puro-CAG-JEDI-2P-Kv
FEATURES             Location/Qualifiers
     source          1..7611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     440..459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(678..782)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        814..1193
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1472..2488
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2412..2434
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2496..2515
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2648..2657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4280..4297)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4286..4510
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4556..4984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4643..4662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4853..4874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4993..5013)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4998..5327
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5178..5313
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5240..5259
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5338..5937
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5338..5357)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5834..5854
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6097..6218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6134..6153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6188..6207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6267..6283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6267..6283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6280..6302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6291..6307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6315..6321,6322..6339,6340..6345))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6360..6381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6498..6515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6669..7257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6749..6768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7428..7611,1..608,609..677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
       61 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      121 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      181 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      241 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      301 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      361 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      421 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      481 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      541 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      601 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      661 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      721 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      781 cgcacatttc cccgaaaagt gccacctgac gtcgacattg attattgact agttattaat
      841 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      901 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      961 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     1021 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     1081 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     1141 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     1201 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     1261 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg gggggcgcgc
     1321 gccrggsggg gsggggsggg gsgrggggsg gggsggggsg rggsggagag gkgcggcggc
     1381 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     1441 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     1501 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     1561 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
     1621 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
     1681 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     1741 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
     1801 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
     1861 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     1921 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
     1981 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     2041 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     2101 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     2161 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     2221 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     2281 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     2341 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     2401 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     2461 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     2521 tcatcatttt ggcaaagaat tcctcgagga attcactcct caggtgcagg ctgcctatca
     2581 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctgcatga
     2641 gctagccgcc accatggaga cgactgtgag gtatgaacag gggtcagagc tcactaaaac
     2701 ttcgagctct ccaacagcag atgagcccac gataaagatt gatgatggtc gtgatgaggg
     2761 taatgaacaa gacagctgtt ccaataccat taggagaaaa atttccccgt ttgtgatgtc
     2821 atttggattc agagtatttg gagttgtgct tatcattgta gacatcatag tggtgattgt
     2881 ggatctggcc atcagtgaga agaaaagagg cattagagag attcttgaag gtgtttccct
     2941 ggctatagca ctcttcttcc ttgttgatgt tctcatgaga gtgtttgttg aaggcttcaa
     3001 gaactatttc cggtccaaac tggttacttt ggatgcagtc atagtagtgg gcactctgct
     3061 aattaatatg acctactcct tctctgacct tgctgccttt aacagcgaga acgtgtatat
     3121 taccgcggat aaacagaaaa acggcattaa agcgaacttt accgtgcgcc ataacgtgga
     3181 agatggcagc gtgcagctgg cggatcatta tcagcagaac accccgattg gcgatggccc
     3241 ggtgctgctg ccggataacc attatctgag ccaccagacc gtgctgagca aagatccgaa
     3301 cgaaaaacgc gatcacatgg tgctgctgga atttgtgacc gcagcgggca ttacacacgg
     3361 catggatgaa ctgtatggcg gcaccggcgg cagcgcgagc cagggcgaag aactgtttac
     3421 cggcgtggtg ccgattctgg tggaactgga tggcgatgtg aacggccata aatttagcgt
     3481 gcgcggcgaa ggcgaaggcg atgcgaccat tggcaaactg accctgaaat ttatttgcac
     3541 caccggcaaa ctaccggtgc cgtggccgac cctggtgacc accttaacct atggcgtgca
     3601 gtgctttagc cgctatccgg atcatatgaa acgccatgat ttttttaaaa gcgcgatgcc
     3661 ggaaggctat gtgcaggaac gcaccattag ctttaaagat gatggcaaat ataaaacccg
     3721 cgcggtggtg aaatttgaag gcgataccct ggtgaaccgc attgaactga aaggcaccga
     3781 ttttaaagaa gatggcaaca ttctggggca taaactggaa tatgatacag atcagatgcc
     3841 gcacatggtt actcttcttc gagttctgaa aattgttatc ttaataagaa tatttcgcct
     3901 ggcttcacag aagaaacaac ttgaagtggt aaccggttct tcaggttcat ctggatcttc
     3961 cacgcgtcag tcccagccca tcctcaacac caaggagatg gccccgcaga gcaagcctcc
     4021 agaggagctg gagatgagca gcatgcccag ccccgtggcc cctctgcccg cacgcacgga
     4081 gggcgtcatc gacatgcgga gcatgtccag cattgacagc ttcatcagct gtgccacgga
     4141 cttccctgaa gccaccagat tctaatgaaa gcttggtacc gagctcggat ccactagtcc
     4201 agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
     4261 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     4321 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     4381 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     4441 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4501 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4621 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4741 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4861 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4921 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4981 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5041 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5101 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5161 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5221 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     5281 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgcatg
     5341 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5401 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5461 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5521 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5581 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5641 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5701 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5761 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5821 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5881 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgattc
     5941 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6001 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6061 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6121 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6181 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6241 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6301 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6361 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6421 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6481 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6541 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6601 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6661 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6721 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6781 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6841 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6901 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6961 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7021 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7081 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7141 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7201 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7261 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7321 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7381 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7441 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7501 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7561 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca g
//
