LOCUS       sequence-35372-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Kaizuka T, Hara T, Oshiro N, Kikkawa U, Yonezawa K, Takehana K, 
            Iemura S, Natsume T, Mizushima N
  TITLE     Tti1 and Tel2 are critical factors in mammalian target of rapamycin 
            complex assembly.
  JOURNAL   J Biol Chem. 2010 Jun 25. 285(26):20109-16.
  PUBMED    20427287
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jun 25. 285(26):20109-16."
COMMENT     Sequence Label: p3xFLAG-CMV10-hTel2
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
       61 cgattacaag gatgacgatg acaagcttgc ggccgcgaat tcatcgatag atctgatgga
      121 gccagcaccc tcagaggttc gactcgccgt ccgggaagcc attcatgccc tctcgtcttc
      181 ggaggatggc ggccacatct tctgcaccct ggagtccctg aagcggtatc tcggtgagat
      241 ggagcctcca gcgctcccga gggagaagga ggagtttgcc tcggcccact tctcgcctgt
      301 cctcagatgt cttgccagca ggctgagccc agcctggctg gagctgctgc cccatggccg
      361 cctggaggag ctgtgggcca gcttcttcct ggagggcccg gcggaccaag ccttcctggt
      421 gttgatggag accatcgagg gtgctgcggg ccccagcttc cggctgatga agatggcgcg
      481 gctgctggcc agattcctgc gcgagggccg gctggcagtg ctgatggagg cgcagtgtcg
      541 gcagcagacg cggcccggct tcatcctgct ccgggagacg ctgctgggca aggtggtggc
      601 cctgcccgat cacctgggca accgcctgca gcaggagaac ttggccgagt tcttccccca
      661 gaactacttc cgcctgctcg gcgaggaggt cgtccgggtg ctgcaggcgg ttgtggactc
      721 tctccaaggt ggcctggatt cctccgtgtc cttcgtgtct caggtccttg ggaaagcctg
      781 tgtccacggg aggcagcagg agatcctggg cgtgctggta ccccggctgg cagcgctcac
      841 ccagggcagc tacctgcacc agcgcgtctg ctggcgcctg gtgg
//
