LOCUS       sequence-353725-        5832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV 
            production vector for neuron -specific expression using the promoter
            hSyn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, 
            Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, 
            Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, 
            Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias
            AS, Reimer J, Dieudonne S, St-Pierre F
  TITLE     Sustained deep-tissue voltage recording using a fast indicator 
            evolved for two-photon microscopy.
  JOURNAL   Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013.
  PUBMED    35985322
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013."
COMMENT     Sequence Label: pAAV-hSyn-JEDI-2P-WPRE
FEATURES             Location/Qualifiers
     source          1..5832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      73..661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     562..581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   723..852
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        897..1344
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      1376..1385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2681..3269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2734..2754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3152..3163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3301..3777
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3500..3519)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3817..3957
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3817..3946
                     /label=AAV2 ITR
     rep_origin      4032..4487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4119..4138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4329..4350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4504..4523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4623..4645
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4683..4701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4769..4873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4874..4942,4943..5734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5092..5111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
       61 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      721 gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct
      781 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
      841 ctaggggttc ctgcggccgc acgcgtgtgt ctagactgca gagggccctg cgtatgagtg
      901 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
      961 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
     1021 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
     1081 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
     1141 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
     1201 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
     1261 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
     1321 gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga tcttctagag tcgacgccac
     1381 catggagacg actgtgaggt atgaacaggg gtcagagctc actaaaactt cgagctctcc
     1441 aacagcagat gagcccacga taaagattga tgatggtcgt gatgagggta atgaacaaga
     1501 cagctgttcc aataccatta ggagaaaaat ttccccgttt gtgatgtcat ttggattcag
     1561 agtatttgga gttgtgctta tcattgtaga catcatagtg gtgattgtgg atctggccat
     1621 cagtgagaag aaaagaggca ttagagagat tcttgaaggt gtttccctgg ctatagcact
     1681 cttcttcctt gttgatgttc tcatgagagt gtttgttgaa ggcttcaaga actatttccg
     1741 gtccaaactg gttactttgg atgcagtcat agtagtgggc actctgctaa ttaatatgac
     1801 ctactccttc tctgaccttg ctgcctttaa cagcgagaac gtgtatatta ccgcggataa
     1861 acagaaaaac ggcattaaag cgaactttac cgtgcgccat aacgtggaag atggcagcgt
     1921 gcagctggcg gatcattatc agcagaacac cccgattggc gatggcccgg tgctgctgcc
     1981 ggataaccat tatctgagcc accagaccgt gctgagcaaa gatccgaacg aaaaacgcga
     2041 tcacatggtg ctgctggaat ttgtgaccgc agcgggcatt acacacggca tggatgaact
     2101 gtatggcggc accggcggca gcgcgagcca gggcgaagaa ctgtttaccg gcgtggtgcc
     2161 gattctggtg gaactggatg gcgatgtgaa cggccataaa tttagcgtgc gcggcgaagg
     2221 cgaaggcgat gcgaccattg gcaaactgac cctgaaattt atttgcacca ccggcaaact
     2281 accggtgccg tggccgaccc tggtgaccac cttaacctat ggcgtgcagt gctttagccg
     2341 ctatccggat catatgaaac gccatgattt ttttaaaagc gcgatgccgg aaggctatgt
     2401 gcaggaacgc accattagct ttaaagatga tggcaaatat aaaacccgcg cggtggtgaa
     2461 atttgaaggc gataccctgg tgaaccgcat tgaactgaaa ggcaccgatt ttaaagaaga
     2521 tggcaacatt ctggggcata aactggaata tgatacagat cagatgccgc acatggttac
     2581 tcttcttcga gttctgaaaa ttgttatctt aataagaata tttcgcctgg cttcacagaa
     2641 gaaacaactt gaagtggtaa cctaatgaaa gcttatcgat aatcaacctc tggattacaa
     2701 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2761 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2821 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2881 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2941 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3001 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3061 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat
     3121 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3181 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3241 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     3301 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     3361 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     3421 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     3481 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3541 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3601 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3661 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3721 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3781 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3841 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3901 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3961 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4021 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4081 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     4141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     4261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     4381 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     4441 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4501 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4621 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4681 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4741 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4801 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4861 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4921 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4981 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5041 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5101 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5161 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5221 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5281 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5341 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5401 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5461 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5521 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5581 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5641 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5701 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5821 tgataatctc at
//
