LOCUS       sequence-353734-        7624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-inactivating retrovirus vector containing a Blasticidin 
            S-resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Fujii H
  TITLE     pSIR-bsr, a self-inactivating retrovirus vector expressing the 
            blasticidin S-resistance gene.
  JOURNAL   Biol Methods Protoc. 2021 Dec 6;6(1):bpab022. doi: 
            10.1093/biomethods/bpab022. eCollection 2021.
  PUBMED    34926829
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Methods Protoc."; date: "2021-12-6"; volume: "6(1):bpab022. doi"; 
            pages: " 10.1093/biomethods/bpab022. eCollection 2021"
COMMENT     Sequence Label: pSIR-bsr
FEATURES             Location/Qualifiers
     source          1..7624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             179..648
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    713..1070
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1137..1553
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1483..1505
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1521..1537
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4666..4682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4666..4682)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4679..4701)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4690..4706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4714..4720,4721..4738,4739..4744))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4759..4780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4897..4914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5068..5656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5148..5167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5827..6618,6619..6687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6450..6469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6688..6792)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6860..6878
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6916..6938)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7038..7057
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7251..7273
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7265..7282
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7266..7282
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgccagtca tattaattac tagtcaatta gttgattttt atttttgaca tatacatgtg
       61 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
      121 aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct
      181 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      241 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
      301 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
      361 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
      421 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
      481 aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta
      541 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
      601 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
      661 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
      721 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc
      781 gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
      841 tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg
      901 gcccgacctg agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag
      961 gagggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa
     1021 tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt
     1081 ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgcgggcc
     1141 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1201 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1261 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1321 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1381 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1441 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1501 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccaaaccta
     1561 aacctcaagt tctttctgac agtggggggc cgctcatcga cctacttaca gaagaccccc
     1621 cgccttatag ggacccaaga ccaccccctt ccgacaggga cggaaatggt ggagaagcga
     1681 cccctgcggg agaggcaccg gacccctccc caatggcatc tcgcctacgt gggagacggg
     1741 agccccctgt ggccgactcc actacctcgc aggcattccc cctccgcgca ggaggaaacg
     1801 gacagcttca atactggccg ttctcctctt ctgaccttta caactggaaa aataataacc
     1861 cttctttttc tgaagatcca ggtaaactga cagctctgat cgagtctgtc ctcatcaccc
     1921 atcagcccac ctgggacgac tgtcagcagc tgttggggac tctgctgacc ggagaagaaa
     1981 aacaacgggt gctcttagag gctagaaagg cggtgcgggg cgatgatggg cgccccactc
     2041 aactgcccaa tgaagtcgat gccgcttttc ccctcgagaa gcttgtcgac ggatccgaat
     2101 tcgtcgaggg aagaaataat aaagacatta aaatcacttt tgtaaacttt ttaaataagg
     2161 gaaattgatt aaaacaacgc gaagttgtaa aatgccatat actgcataca ttttgtggga
     2221 aaaaatgtgc atttacctgc tcaccaaatt atcttattag ctagcagtag agctctttgt
     2281 gtgatgggaa gatgggataa cagctgttgt tactgtactg gttttttttt ttaatatatt
     2341 taaaaaaaag agtgtctcct aaagtgtaag cgctatcatg ctggactaat gttttaattt
     2401 aatttaattg ttgtcttggt agagatgggg tctcgctatt ttgctcacgc agatctcaaa
     2461 ctcctgacgc ccaggtacac tcccatctcg gcctcctaaa atggtgggat cacagacgtc
     2521 agccaccgag cccggtcact tttttgtatt ccccacagta ttgatgtata tcttctgcgt
     2581 tcaaaagcaa ttttttaaag cctcataacg tggtaacaga atactttgca cattacaaaa
     2641 ttcagaacac ggaaacaaga agctcgcttt tttttccccc ctattttggt ttggcccttt
     2701 agatttcccc tcccccaccg gggcgggact tcccgccgac ttctttcagg ttctcagttc
     2761 ggtccgccaa ctgtcgtata aaggcgctgc ctcaggccag aggcctcaca aagcgcgtcg
     2821 agacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2881 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     2941 cgatctgatc accatgaaaa catttaacat ttctcaacaa gatctagaat tagtagaagt
     3001 agcgacagag aagattacaa tgctttatga ggataataaa catcatgtgg gagcggcaat
     3061 tcgtacgaaa acaggagaaa tcatttcggc agtacatatt gaagcgtata taggacgagt
     3121 aactgtttgt gcagaagcca ttgcgattgg tagtgcagtt tcgaatggac aaaaggattt
     3181 tgacacgatt gtagctgtta gacaccctta ttctgacgaa gtagatagaa gtattcgagt
     3241 ggtaagtcct tgtggtatgt gtagggagtt gatttcagac tatgcaccag attgttttgt
     3301 gttaatagaa atgaatggca agttagtcaa aactacgatt gaagaactca ttccactcaa
     3361 atatacccga aattaaggct ggatgatcct ccagcgcggg gatctcatgc tggagatccc
     3421 gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca
     3481 acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt
     3541 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     3601 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
     3661 aaggtcagga acagatggaa cagggtcgac cggtcgaccg gtcgacccta gagaaccatc
     3721 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     3781 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     3841 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     3901 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     3961 ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa
     4021 ttaatttggt tttttttctt aagtatttac attaaatggc catagtactt aaagttacat
     4081 tggcttcctt gaaataaaca tggagtattc agaatgtgtc ataaatattt ctaattttaa
     4141 gatagtatct ccattggctt tctacttttt cttttatttt tttttgtcct ctgtcttcca
     4201 tttgttgttg ttgttgtttg tttgtttgtt tgttggttgg ttggttaatt tttttttaaa
     4261 gatcctacac tatagttcaa gctagactat tagctactct gtaacccagg gtgaccttga
     4321 agtcatgggt agcctgctgt tttagccttc ccacatctaa gattacaggt atgagctatc
     4381 atttttggta tattgattga ttgattgatt gatgtgtgtg tgtgtgattg tgtttgtgtg
     4441 tgtgattgtg tatatgtgtg tatggttgtg tgtgattgtg tgtatgtatg tttgtgtgtg
     4501 attgtgtgtg tgtgattgtg catgtgtgtg tgtgtgattg tgtttatgtg tatgatgtgt
     4561 tgtgtatata tatttatggt agtgagaggc aacgctccgg ctcaggtgtc aggttggttt
     4621 ttgagacaga gtctttcact tagcttggaa ttaattcgta atcatgtcat agctgtttcc
     4681 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4741 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4801 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4861 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6841 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6901 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6961 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7021 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7081 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     7141 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     7201 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     7261 acgttgtaaa acgacggcca gtgccaagct agctttgctc ttaggagttt cctaatacat
     7321 cccaaactca aatatataaa gcatttgact tgttctatgc cctagggggc ggggggaagc
     7381 taagccagct ttttttaaca tttaaaatgt taattccatt ttaaatgcac agatgttttt
     7441 atttcataag ggtttcaatg tgcatgaatg ctgcaatatt cctgttacca aagctagtat
     7501 aaataaaaat agataaacgt ggaaattact tagagtttct gtcattaacg tttccttcct
     7561 cagttgacaa cataaatgcg ctgctgagaa gccagtttgc atctgtcagg atcaatttcc
     7621 catt
//
