LOCUS       sequence-353736-       10498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1013
FEATURES             Location/Qualifiers
     source          1..10498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1760..1771
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    1778..1789
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             2837..2848
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3436..4024
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3489..3509)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3907..3918)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4096..4329
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4407..4528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4444..4463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4498..4517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4568..4703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4723..4742)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4724..4742)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4752..4769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4752..4768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4761..4783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4910..5365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4997..5016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5207..5228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5391..5495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5496..5564,5565..6356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5714..5733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6527..7115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7016..7035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7269..7286
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7403..7424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7439..7444,7445..7462,7463..7469)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7477..7493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7482..7504
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7501..7517
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7501..7517
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7536..7556
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7538..7556
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7584..7810
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7811..7991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8038..8163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8656..8889
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9074..9118
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9349..9559
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9371..9567
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9418..9553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9599..10201
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9599..9618)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10095..10115
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10244..10361
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggt
     1201 gaagctcgcg aaggcaggta aaaatcaagg tgaccccaag aaaatggctc ctcctccaaa
     1261 ggaggtagaa gaagatagtg aagatgagga aatgtcagaa gatgaagaag atgatagcag
     1321 tggagaagag gtcgtcatac ctcagaagaa aggcaagaag gctgctgcaa cctcagcaaa
     1381 gaaggtggtc gtttccccaa caaaaaaggt tgcagttgcc acaccagcca agaaagcagc
     1441 tgtcactcca ggcaaaaagg cagcagcaac acctgccaag aagacagtta caccagccaa
     1501 agcagttacc acacctggca agaagggagc cacaccaggc aaagcattgg tagcaactcc
     1561 tggtaagaag ggtgctgcca tcccagccaa gggggcaaag aatggcaaga atgccaagaa
     1621 ggaagacagt gatgaagagg aggatgatga cagtgaggag gatgaggagg atgacgagga
     1681 cgaggatgag gatgaagatg aaattgaacc agcagcgatg aaagcagcag ctgctgcccc
     1741 tgcctcagag gatgaggacg atgaggatga cgaagatgat gaggatgacg atgacgatga
     1801 ggaagatgac tctgaagaag aagctatgga gactacacca gccaaaggaa agaaagctgc
     1861 aaaagttgtt cctgtgaaag ccaagaacgt ggctgaggat gaagatgaag aagaggatga
     1921 tgaggacgag gatgacgacg acgacgaaga tgatgaagat gatgatgatg aagatgatga
     1981 ggaggaggaa gaagaggagg aggaagagcc tgtcaaagaa gcacctggaa aacgaaagaa
     2041 ggaaatggcc aaacagaaag cagctcctga agccaagaaa cagaaagtgg aaggcacaga
     2101 accgactacg gctttcaatc tctttgttgg aaacctaaac tttaacaaat ctgctcctga
     2161 attaaaaact ggtatcagcg atgtttttgc taaaaatgat cttgctgttg tggatgtcag
     2221 aattggtatg actaggaaat ttggttatgt ggattttgaa tctgctgaag acctggagaa
     2281 agcgttggaa ctcactggtt tgaaagtctt tggcaatgaa attaaactag agaaaccaaa
     2341 aggaaaagac agtaagaaag agcgagatgc gagaacactt ttggctaaaa atctccctta
     2401 caaagtcact caggatgaat tgaaagaagt gtttgaagat gctgcggaga tcagattagt
     2461 cagcaaggat gggaaaagta aagggattgc ttatattgaa tttaagacag aagctgatgc
     2521 agagaaaacc tttgaagaaa agcagggaac agagatcgat gggcgatcta tttccctgta
     2581 ctatactgga gagaaaggtc aaaatcaaga ctatagaggt ggaaagaata gcacttggag
     2641 tggtgaatca aaaactctgg ttttaagcaa cctctcctac agtgcaacag aagaaactct
     2701 tcaggaagta tttgagaaag caacttttat caaagtaccc cagaaccaaa atggcaaatc
     2761 taaagggtat gcatttatag agtttgcttc attcgaagac gctaaagaag ctttaaattc
     2821 ctgtaataaa agggaaattg agggcagagc aatcaggctg gagttgcaag gacccagggg
     2881 atcacctaat gccagaagcc agccatccaa aactctgttt gtcaaaggcc tgtctgagga
     2941 taccactgaa gagacattaa aggagtcatt tgacggctcc gttcgggcaa ggatagttac
     3001 tgaccgggaa actgggtcct ccaaagggtt tggttttgta gacttcaaca gtgaggagga
     3061 tgccaaagct gccaaggagg ccatggaaga cggtgaaatt gatggaaata aagttacctt
     3121 ggactgggcc aaacctaagg gtgaaggtgg cttcgggggt cgtggtggag gcagaggcgg
     3181 ctttggagga cgaggtggtg gtagaggagg ccgaggagga tttggtggca gaggccgggg
     3241 aggctttgga gggcgaggag gcttccgagg aggcagagga ggaggaggtg accacaagcc
     3301 acaaggaaag aagacgaagt ttgaatagta atgaactagt ccgtaacttg aaagtatttc
     3361 gatttcttgg ctttatatat cttgtggaaa ggacgagtag tgtttgtctt aacttatgcc
     3421 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3481 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3541 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3601 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3661 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3721 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3781 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3841 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3901 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3961 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4021 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4081 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4141 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4201 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4261 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4321 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4381 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     4441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4501 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4561 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4621 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4681 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4741 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4801 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4861 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4921 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4981 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5041 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5101 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5161 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5221 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5281 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5341 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6001 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6661 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6901 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7141 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7201 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7261 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7321 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     7381 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7441 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7501 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     7561 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7621 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     7681 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7741 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7801 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7861 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7921 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7981 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     8041 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     8101 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     8161 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     8221 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     8281 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     8341 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     8401 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     8461 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     8521 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8581 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8641 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8701 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8761 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8821 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8881 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8941 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     9001 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     9061 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     9121 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     9181 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     9241 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     9301 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9361 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9541 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     9601 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     9661 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     9721 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     9781 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     9841 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9901 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9961 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    10021 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    10081 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    10141 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
    10201 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
    10261 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10321 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    10381 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
    10441 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
