LOCUS       sequence-353738-        9511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3233
FEATURES             Location/Qualifiers
     source          1..9511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2265..2289)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2297..2338
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2449..3037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2502..2522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2920..2931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3109..3342
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3420..3541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3457..3476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3511..3530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3581..3716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3736..3755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3737..3755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3765..3782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3765..3781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3774..3796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3923..4378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4010..4029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4220..4241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4404..4508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4509..4577,4578..5369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4727..4746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5540..6128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6029..6048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6282..6299
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6416..6437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6452..6457,6458..6475,6476..6482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6490..6506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6495..6517
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6514..6530
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6514..6530
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6549..6569
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6551..6569
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6597..6823
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6824..7004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7051..7176
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7669..7902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8087..8131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8362..8572
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8384..8580
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8431..8566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8612..9214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8612..8631)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9108..9128
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9257..9374
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtgcac taaaatggaa cagcccttct accacgacga
     1261 ctcatacaca gctacgggat acggccgggc ccctggtggc ctctctctac acgactacaa
     1321 actcctgaaa ccgagcctgg cggtcaacct ggccgacccc taccggagtc tcaaagcgcc
     1381 tggggctcgc ggacccggcc cagagggcgg cggtggcggc agctactttt ctggtcaggg
     1441 ctcggacacc ggcgcgtctc tcaagctcgc ctcttcggag ctggaacgcc tgattgtccc
     1501 caacagcaac ggcgtgatca cgacgacgcc tacacccccg ggacagtact tttacccccg
     1561 cgggggtggc agcggtggag gtgcaggggg cgcagggggc ggcgtcaccg aggagcagga
     1621 gggcttcgcc gacggctttg tcaaagccct ggacgatctg cacaagatga accacgtgac
     1681 accccccaac gtgtccctgg gcgctaccgg ggggcccccg gctgggcccg ggggcgtcta
     1741 cgccggcccg gagccacctc ccgtttacac caacctcagc agctactccc cagcctctgc
     1801 gtcctcggga ggcgccgggg ctgccgtcgg gaccgggagc tcgtacccga cgaccaccat
     1861 cagctacctc ccacacgcgc cgcccttcgc cggtggccac ccggcgcagc tgggcttggg
     1921 ccgcggcgcc tccaccttca aggaggaacc gcagaccgtg ccggaggcgc gcagccggga
     1981 cgccacgccg ccggtgtccc ccatcaacat ggaagaccaa gagcgcatca aagtggagcg
     2041 caagcggctg cggaaccggc tggcggccac caagtgccgg aagcggaagc tggagcgcat
     2101 cgcgcgcctg gaggacaagg tgaagacgct caaggccgag aacgcggggc tgtcgagtac
     2161 cgccggcctc ctccgggagc aggtggccca gctcaaacag aaggtcatga cccacgtcag
     2221 caacggctgt cagctgctgc ttggggtcaa gggacacgcc ttctgcccaa ctttcttgta
     2281 caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta
     2341 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     2401 aaaggacgaa acgtcatggc gtaaccggcg gtcacgcgtt aagtcgacaa tcaacctctg
     2461 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2521 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2581 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2641 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2701 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2761 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2821 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2881 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2941 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3001 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     3061 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3121 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     3181 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     3241 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     3301 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     3361 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     3421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3541 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     3601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3721 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     3781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3901 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4261 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4321 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5221 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6181 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6241 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6301 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6361 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6421 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6481 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6541 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     6601 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     6661 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     6721 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     6781 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     6841 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6901 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6961 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7021 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7081 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7141 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7201 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7261 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7321 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7381 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7441 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7501 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     7561 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     7621 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     7681 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     7741 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     7801 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     7861 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     7921 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     7981 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8041 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8101 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8161 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     8221 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     8281 ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt
     8341 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca
     8401 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8461 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8521 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8581 aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     8641 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     8701 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     8761 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     8821 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     8881 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     8941 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9001 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9061 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9121 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     9181 ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg
     9241 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     9301 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     9361 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
     9421 aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
     9481 gaccttctag gtcttgaaag gagtgggaat t
//
