LOCUS       sequence-353741-       11755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1797
FEATURES             Location/Qualifiers
     source          1..11755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4693..5281
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4746..4766)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5164..5175)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5353..5586
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5664..5785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5701..5720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5755..5774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5825..5960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5980..5999)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5981..5999)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6009..6026)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6009..6025)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6018..6040)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6167..6622
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6254..6273)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6464..6485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6648..6752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6753..6821,6822..7613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6971..6990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7784..8372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8273..8292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8526..8543
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8660..8681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8696..8701,8702..8719,8720..8726)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8734..8750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8739..8761
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8758..8774
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8758..8774
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8793..8813
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8795..8813
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8841..9067
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9068..9248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9295..9420
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9913..10146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10331..10375
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10606..10816
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10628..10824
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10675..10810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10856..11458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10856..10875)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11352..11372
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11501..11618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 tgccacacgg aagccaatga gatatgggca tacagaggga cacacggagg tctgttttga
     1261 tgattctggg agttttattg tgacttgtgg aagtgatggt gatgtgagga tttgggaaga
     1321 cttggatgat gatgatccta agttcattaa tgttggagaa aaggcatatt catgtgcttt
     1381 gaagagtgga aaactggtca ctgcagtttc taataatact attcaagtcc acacatttcc
     1441 tgaaggagtt ccagatggta tattgactcg cttcactaca aatgcaaacc atgtggtctt
     1501 taatggggat ggtactaaaa ttgctgctgg atctagtgat tttctagtca aaattgtgga
     1561 tgtgatggat agcagccaac agaaaacatt tcgaggacat gatgcccctg ttttaagtct
     1621 ttcctttgat cctaaggaca tctttctggc atcagctagt tgtgatggat ctgtcagagt
     1681 gtggcaaatt tcagatcaga catgtgctat tagttggcca ctgctacaaa aatgcaacga
     1741 tgtgataaat gcaaaatcaa tctgcagact tgcttggcag ccaaaaagtg ggaagttact
     1801 ggcaattcct gtggaaaaat ctgttaagct atatagaaga gaatcttgga gtcatcaatt
     1861 tgatctttca gataatttca tctctcagac cctcaatata gtaacctggt ctccctgtgg
     1921 gcaatattta gctgcaggta gtattaatgg tctaatcata gtttggaatg tggaaaccaa
     1981 agactgcatg gaaagggtga aacatgagaa aggttatgca atttgtggtc tggcatggca
     2041 tcctacttgt ggtcgaatat cgtatactga tgcggaagga aatctagggc ttctagagaa
     2101 tgtttgtgac cccagtggaa agacatcaag cagtaaggta tctagcagag tggaaaagga
     2161 ttataatgat ctttttgatg gagatgatat gagtaatgct ggtgattttc taaatgacaa
     2221 tgcagttgag atcccttctt tttcaaaagg gattataaat gatgatgagg atgatgaaga
     2281 cctcatgatg gcttcaggtc gtcctagaca gcgaagtcac atcctagaag atgatgaaaa
     2341 ctcagttgat atttcaatgc taaaaactgg ttctagtctt ctcaaagagg aggaggaaga
     2401 tggtcaagaa ggcagcattc acaatctacc acttgtaaca tcccaaaggc cattttatga
     2461 tggacccatg ccaactcccc ggcaaaagcc atttcagtca ggttctacac cgttgcatct
     2521 cactcacaga ttcatggtgt ggaactctat tggaattatt cgctgctata atgatgagca
     2581 agacaatgcc atagatgtgg agttccatga tacctccata caccatgcaa cacacttatc
     2641 aaacactttg aattatacaa tagcagatct ttcccacgaa gctattttgt tggcatgtga
     2701 aagcactgat gaactagcaa gcaagcttca ctgcctgcac tttagttctt gggattcaag
     2761 caaagagtgg ataatagact tgcctcagaa tgaggatatt gaagccatat gtctcggtca
     2821 aggatgggct gctgccgcta ctagtgccct gcttcttcga ttgtttacta ttggaggggt
     2881 tcaaaaagag gtattcagcc ttgctggacc tgtggtgtca atggcaggac atggagaaca
     2941 gcttttcatt gtttatcaca gaggtacagg atttgatggg gatcagtgcc ttggagttca
     3001 actgctagag ctggggaaaa agaaaaaaca aattttgcat ggtgaccctc ttcctcttac
     3061 aaggaaatcc taccttgcat ggattgggtt ttcagctgaa ggtacccctt gttacgtgga
     3121 ttcagaagga attgttcgaa tgcttaacag aggacttggt aatacgtgga ctcctatatg
     3181 taatacaaga gagcactgca aaggaaaatc tgatcactac tgggtggttg gtatccatga
     3241 aaatccccag caactaaggt gcattccttg taaaggttct cggtttcccc caacccttcc
     3301 acgccctgct gttgctatat tatcctttaa gcttccttac tgtcagattg caacagagaa
     3361 aggacaaatg gaggagcaat tttggcgttc agttatattt cacaaccacc ttgattattt
     3421 agctaaaaat ggttatgaat atgaagagag cactaaaaat caagcaacaa aagagcaaca
     3481 ggaactttta atgaaaatgc ttgcgctttc ttgtaaactg gagcgagaat tccgttgtgt
     3541 ggaacttgct gatctaatga ctcaaaatgc tgtgaattta gccattaaat atgcttctcg
     3601 ctctcggaaa ttaatactgg ctcaaaaact aagtgaactg gctgtagaga aggcagccga
     3661 attgacagca acccaggtgg aagaggaaga agaagaagaa gatttcagaa aaaagctgaa
     3721 tgctggttac agcaatactg ctacagagtg gagccaacca aggttcagaa atcaagttga
     3781 agaagatgct gaggacagtg gagaagctga tgatgaagaa aaaccagaaa tacataagcc
     3841 tggacagaac tcgttttcca aaagtacaaa ttcctctgat gtttcagcta agtcaggtgc
     3901 agttaccttt agcagccaag gacgagtaaa tccctttaag gtatcagcca gttccaaaga
     3961 accagccatg tcaatgaatt cagcacgttc aactaatatt ttagacaata tgggcaaatc
     4021 atccaagaaa tccactgcac ttagtcgaac tacaaataat gaaaagtctc ccattataaa
     4081 gcctctgatt ccaaagccga agcctaagca ggcatctgca gcatcctatt tccagaaaag
     4141 aaattctcaa actaataaaa ctgaggaagt gaaagaagaa aatcttaaaa atgtattatc
     4201 tgaaacccca gctatatgtc ctcctcaaaa cactgaaaac caaaggccaa agaccgggtt
     4261 ccagatgtgg ttagaagaaa atagaagtaa tattttgtct gacaatcctg acttttcaga
     4321 tgaagcagac ataataaaag aaggaatgat tcgatttaga gtattgtcaa ctgaagaaag
     4381 aaaggtgtgg gctaacaaag ccaaaggaga aacggcaagt gaaggaactg aagcaaagaa
     4441 gcgaaaacgt gtggttgatg aaagtgatga aacagaaaac caggaagaaa aagcaaaaga
     4501 gaacctgaat ttgtctaaaa agcagaaacc tttagatttt tctacaaatc agaaactatc
     4561 agcttttgca tttaagcagg agtagtaatg aactagtccg taacttgaaa gtatttcgat
     4621 ttcttggctt tatatatctt gtggaaagga cgacttacac acgttcatgt gtcgttgacg
     4681 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4741 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4801 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4861 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4921 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4981 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5041 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5101 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5161 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5221 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5281 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5341 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5401 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5461 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5521 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5581 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     5641 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     5701 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5761 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     5821 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5881 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5941 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6001 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6061 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6361 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6481 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6601 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6721 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6781 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6841 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6901 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6961 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7021 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7081 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7141 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7201 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7261 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7321 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7381 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7441 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7501 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7561 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7621 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7681 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7921 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8461 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8521 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8581 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8641 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8701 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8761 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     8821 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     8881 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     8941 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     9001 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     9061 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9121 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9181 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9241 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     9301 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     9361 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     9421 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     9481 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     9541 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     9601 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     9661 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     9721 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     9781 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9841 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9901 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     9961 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
    10021 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
    10081 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
    10141 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
    10201 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
    10261 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
    10321 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
    10381 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    10441 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    10501 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
    10561 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    10621 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
    10681 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    10741 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    10801 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
    10861 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
    10921 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
    10981 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
    11041 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
    11101 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    11161 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    11221 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    11281 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    11341 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    11401 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
    11461 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    11521 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    11581 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    11641 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
    11701 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
