LOCUS       sequence-353746-       10024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3220
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2778..2802)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2810..2851
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2962..3550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3015..3035)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3433..3444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3622..3855
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3933..4054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3970..3989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4024..4043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4094..4229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4249..4268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4250..4268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4278..4295)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4278..4294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4287..4309)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4436..4891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4523..4542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4733..4754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4917..5021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5022..5090,5091..5882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5240..5259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6053..6641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6542..6561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6795..6812
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6929..6950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6965..6970,6971..6988,6989..6995)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7003..7019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7008..7030
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7027..7043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7027..7043
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7062..7082
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7064..7082
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7110..7336
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7337..7517
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7564..7689
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8182..8415
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8600..8644
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8875..9085
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8897..9093
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8944..9079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9125..9727
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9125..9144)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9621..9641
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9770..9887
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggctgt ggccctgggt tgtgcaatcc aggcatcctt
     1261 gaatcaaggc tctgtgtttc aagaatatga tactgactgt gaagttttcc gtcagcgctt
     1321 caggcagttc cagtacagag aagcagctgg gcctcatgaa gcatttaaca aactctggga
     1381 gctttgctgt caatggctga agccaaagat gcgctctaag gaacaaatcc tggagctgct
     1441 agtgttggag caattcctaa ctatcctgcc cacagagata gagacctggg tgagggagca
     1501 ctgcccagag aatagagaaa gagttgtgtc actgatagaa gacttacaga gagaacttga
     1561 gataccagag cagcaggttg atatgcatga catgctcttg gaagaactgg caccagtggg
     1621 aacggcacac ataccaccaa ccatgcacct agagtcacct gcactccagg taatgggacc
     1681 tgcccaggag gccccagtag cagaggcatg gatcccacag gcagggccac cggagctgaa
     1741 ctatggtgct actggagaat gtcagaactt tctggaccct ggatatccat taccaaaact
     1801 tgacatgaac ttctcattgg agaatagaga agagccatgg gtgaaggaat tacaggattc
     1861 taaagaaatg aaacaattac ttgattccaa gataggtttt gagatcggga tagaaaatga
     1921 agaagatact tcaaaacaga aaaaaatgga gactatgtat ccatttattg taactttaga
     1981 ggggaatgct ctccagggtc ccattttgca aaaagactat gtacagttag aaaatcaatg
     2041 ggaaaccccc ccagaggatt tacagacaga tttagcaaaa ctggtagatc agcagaaccc
     2101 cactctggga gagacacctg agaactccaa cttggaagaa cctctcaacc ctaaacccca
     2161 caagaaaaag agtccaggag agaaacctca ccgatgtcct cagtgtggaa aatgttttgc
     2221 tcggaagtca caacttactg ggcatcagag aattcattca ggagaagaac ctcacaaatg
     2281 ccctgaatgt gggaaaagat tccttcgtag ttcagacctt tatagacacc aacgacttca
     2341 tacaggggag agaccctatg aatgcactgt atgtaaaaag cgattcactc ggcggtcaca
     2401 tcttataggg caccagagaa cccattctga agaagaaaca tataaatgtc ttgagtgtgg
     2461 gaaaagtttt tgtcatggat caagtcttaa aagacatctg aaaactcata caggtgaaaa
     2521 acctcataga tgtcataatt gtgggaaaag ttttagtcga ctgacagctc ttactttgca
     2581 ccagagaacg catactgaag agagaccttt taaatgtaat tattgtggga aaagttttag
     2641 acagagacca agcctcgtta ttcatttaag aatccacaca ggggagaagc catacaagtg
     2701 tactcattgt tctaaaagct tcagacagag agccggcctt attatgcacc aggtcactca
     2761 ctttagagga cttatttacc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     2821 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag
     2881 tatttcgatt tcttggcttt atatatcttg tggaaaggac gagtaactgg gttacgaccg
     2941 tatgttacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3001 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3061 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3121 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3181 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3241 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3301 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3361 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3421 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3481 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3541 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3601 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3661 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3721 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3781 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3841 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     3901 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     3961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4021 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4081 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4141 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4201 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4261 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4321 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4861 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4921 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4981 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5041 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5101 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5161 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5221 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5281 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5341 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5401 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5461 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5521 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5581 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5641 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5701 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5761 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5821 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5881 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5941 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6001 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6061 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6121 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6181 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6421 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6601 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6661 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6721 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6781 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6841 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6901 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6961 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7021 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7081 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7141 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7201 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7261 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7321 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7381 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7441 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7501 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7561 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7621 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7681 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7741 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7801 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     7861 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7921 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7981 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8041 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8101 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8161 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8221 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8281 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8341 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8401 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8461 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8521 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8581 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8641 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8701 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8761 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     8821 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8881 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8941 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9001 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9061 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9121 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9181 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9241 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9301 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9361 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9421 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9481 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9541 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9601 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9661 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9721 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9781 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9841 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9901 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
     9961 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
    10021 aatt
//
