LOCUS       sequence-353747-        9655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3028
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2409..2433)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2441..2482
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2593..3181
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2646..2666)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3064..3075)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3253..3486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3564..3685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3601..3620)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3655..3674
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3725..3860
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3880..3899)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3881..3899)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3909..3926)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3909..3925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3918..3940)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4067..4522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4154..4173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4364..4385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4548..4652
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4653..4721,4722..5513)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4871..4890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5684..6272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6173..6192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6426..6443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6560..6581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6596..6601,6602..6619,6620..6626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6634..6650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6639..6661
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6658..6674
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6658..6674
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6693..6713
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6695..6713
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6741..6967
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6968..7148
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7195..7320
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7813..8046
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8231..8275
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8506..8716
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8528..8724
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8575..8710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8756..9358
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8756..8775)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9252..9272
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9401..9518
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgatggc tgcaaaagtg gttcctatgc ccccaaagcc
     1261 aaagcagtcc tttatactga gagttccgcc agactccaag ctgggccaag acctacttcg
     1321 agatgccact aacgggccca agaccatcca ccagctagtg ctggagcact tcctcacctt
     1381 cttgcccaag ccaagcctgg tccagcccag tcagaaagtc aaggagacct tggttattat
     1441 gaaagatgtg agctcaagcc ttcagaacag agtgcatcct cgtcccttgg tgaagcttct
     1501 gcccaaagga gtccaaaagg aacaagagac agtgtctctg tatttgaaag ctaaccctga
     1561 ggagctggtg gtctttgagg atttgaatgt atttcactgc caggaagaat gtgtgagctt
     1621 ggatcctact caacaactca cgtcagagaa ggaagatgac agcagtgtcg gggaaatgat
     1681 gttactggca gtcaatggca gtaatcctga aggtgaagat cctgagaggg aacctgtaga
     1741 aaatgaagat tatagagaaa agtcttcaga tgatgatgaa atggattctt ccttggtctc
     1801 tcagcagcct cccgataacc aggaaaagga acgactaaat acatccattc cacaaaaaag
     1861 gaaaatgaga aatctgttag ttaccattga gaatgatact cctctagagg aactctcaaa
     1921 atatgtagac atcagtatta ttgcccttac tcgaaatcgg aggacaagga gatggtacac
     1981 ttgtccactg tgtgggaaac agtttaatga aagttcttac ctcatttccc accagaggac
     2041 ccacactgga gaaaaaccct atgactgtaa tcactgtggg aaaagcttca atcataaaac
     2101 aaacctcaat aaacatgagc gaattcatac aggagagaaa ccttattcct gttctcagtg
     2161 tggaaaaaac ttccgtcaga attctcatcg gagtcgtcat gaaggaatcc atataaggga
     2221 gaagatattt aagtgtccag aatgtgggaa aaccttccca aagaatgagg agtttgtgct
     2281 tcatctgcag agtcatgagg ctgagagacc atatggttgc aaaaaatgtg ggagaagatt
     2341 tggtcggctg tcaaactgta cccggcatga gaaaacccac tcagcctgta agacccgaaa
     2401 gcagaagtac ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc
     2461 tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat
     2521 ttcttggctt tatatatctt gtggaaagga cgactctgca atcatcatat cgttaccacg
     2581 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2641 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2701 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2761 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2821 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2881 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2941 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3001 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3061 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3121 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3181 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3241 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     3301 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3361 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3421 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3481 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     3541 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     3601 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3661 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     3721 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3841 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     3901 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3961 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4021 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     4081 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4141 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4201 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4261 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4321 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4381 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4441 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4501 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4621 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4861 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6541 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6601 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6661 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6721 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     6781 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     6841 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     6901 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     6961 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7021 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7081 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7141 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7201 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7261 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7321 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7381 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7441 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7501 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     7561 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     7621 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     7681 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     7741 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     7801 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     7861 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     7921 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     7981 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8041 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8101 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8161 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8221 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     8281 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     8341 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     8401 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     8461 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8521 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8581 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8641 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8701 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     8761 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8821 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     8881 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8941 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     9001 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9061 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9121 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9181 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9241 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9301 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
     9361 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     9421 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9481 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     9541 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
     9601 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
