LOCUS       sequence-353748-       10909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3121
FEATURES             Location/Qualifiers
     source          1..10909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3847..4435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3900..3920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4318..4329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4507..4740
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4818..4939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4855..4874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4909..4928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4979..5114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5134..5153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5135..5153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5163..5180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5163..5179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5172..5194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5321..5776
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5408..5427)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5618..5639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5802..5906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5907..5975,5976..6767)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6125..6144)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6938..7526
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7427..7446
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7680..7697
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7814..7835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7850..7855,7856..7873,7874..7880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7888..7904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7893..7915
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7912..7928
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7912..7928
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7947..7967
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7949..7967
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7995..8221
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8222..8402
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8449..8574
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9067..9300
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9485..9529
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9760..9970
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9782..9978
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9829..9964
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10010..10612
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10010..10029)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10506..10526
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10655..10772
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 gcaggcgatc tttgaggccc tggagggaat ggacaatcag accgttctgg ctgtccagtc
     1261 attattggat ggccaaggag cagtccctga tccgacaggc cagagtgtca atgcgccccc
     1321 tgctatccag ccattggatg acgaggatgt atttctctgc gggaagtgta agaagcaatt
     1381 caactcgctg ccagcgttta tgacccacaa gcgggaacag tgccagggga atgcccccgc
     1441 cctggccaca gtctcactgg ccaccaacag catctaccca ccttcggcag cacccacagc
     1501 ggtccagcag gccccaactc ctgccaatcg ccagatctcc acatacatca cagtgccccc
     1561 gtccccactg atccagaccc tggtgcaggg gaacatcttg gtgagcgatg atgtgctcat
     1621 gtctgccatg tcagccttca catccctgga ccagcccatg ccccagggcc ccccacctgt
     1681 gcagagcagc ctgaacatgc attccgtgcc cagctacctc acccagcctc cacctcctcc
     1741 tccacctcct ccaccactgc ccccaccgcc accacctcag cctccaccac ctccacccca
     1801 gagcctgggc ccccctgggc gtcccaaccc tggtgggaac ggtgtggtgg aggtgtacag
     1861 tgctgctgcg cccctggctg ggagtggaac ggtggagatc caggcactgg ggatgcagcc
     1921 ctacccaccc ctagaggtgc caaaccagtg tgtggagcct ccagtatatc ccacccccac
     1981 agtgtacagc cctggcaaac agggattcaa acccaaagga ccaaaccccg ccgcccccat
     2041 gaccagcgcc accgggggca cggtggccac ctttgactct ccagcaacgc tgaagacccg
     2101 acgagctaaa gctgcaggga agccaaaggc tcagaaactc aagtgctcat actgtgacaa
     2161 gtcattcacc aaaaactttg acctgcagca gcacatccga agccacaccg gtgagaagcc
     2221 cttccagtgc attgcatgtg gccgtgcctt tgcccagaag tctaatgtta agaaacacat
     2281 gcagacccac aaggtgtggc ctccaggaca cagtggtggc accgtgtctc gaaactctgt
     2341 gaccgtacag gtcatggccc tgaaccccag caggcaggag gacgaggaaa gcacagggtt
     2401 gggccagccc ctgccgggtg cgccacagcc ccaggccttg tccacagctg gtgaggaaga
     2461 gggggacaag ccggagtcca agcaggtggt cctcatcgac agctcctacc tgtgccaatt
     2521 ctgccccagc aaattcagca cctacttcca gctcaagtct cacatgaccc agcataagaa
     2581 tgagcaggtg tacaagtgtg tggtcaaaag ctgtgcccag acgttcccaa agctcgacac
     2641 atttctggag cacatcaaga gccaccagga ggagctgagc taccgctgcc acctctgcgg
     2701 caaggacttc ccctcgctgt acgacctggg cgtgcaccag tactcccaca gcctcctgcc
     2761 acagcacagc cccaagaagg acaatgccgt ctacaagtgt gtcaaatgtg tcaacaaata
     2821 ctccacccct gaggccctgg agcaccacct gcagaccgcc actcacaact tcccctgccc
     2881 acactgccag aaggtgtttc cttgtgaacg ctacctgcgg cgtcatctgc ccacccacgg
     2941 cagcgggggc aggttcaagt gccaagtgtg caagaagttc ttccggcggg agcattatct
     3001 caaactgcat gctcacatcc actcgggtga gaagccctac aaatgctcag tgtgcgagtc
     3061 tgcgttcaac cgcaaggaca aactgaagag acacatgttg atccacgagc ccttcaagaa
     3121 atacaaatgc cctttctcga cgcacacagg ctgcagtaag gagttcaacc ggccggacaa
     3181 gctgaaggcc cacatcctct cccactctgg catgaagctc cacaaatgcg ccctgtgcag
     3241 caagtccttc agccgccgtg cccacctcgc cgagcatcag cgcgcccaca cgggcaacta
     3301 caagttccgc tgtgctggct gcgccaaggg cttttcccgc cacaaatacc tcaaagatca
     3361 ccgctgtcgt ctcggccccc aaaaggacaa ggacctgcaa acccggcggc ccccccagag
     3421 gagggcagcc ccccgcagtt gcggcagtgg tgggcgcaag gtgctgaccc ccttgcctga
     3481 cccgctgggg ctggaggagc tgaaggacac aggggctggg ctggtgcccg aggctgtccc
     3541 cggcaagccg cccttcgcag agccggacgc ggtgctgtcc atcgttgtgg gtggtgcggt
     3601 gggcgcggaa actgagctgg tggtacctgg acacgctgag gggctgggct ccaacctggc
     3661 tctggcggag ctgcaggctg gggccgaggg cccatgtgcc atgctcgctg tgcccgtcta
     3721 catccaggcc tccgagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     3781 gctttatata tcttgtggaa aggacgacaa cgtcactcac gccatctttt cacgcgttaa
     3841 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4381 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     4441 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4501 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4561 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4621 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4681 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4741 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4801 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4921 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4981 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5041 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5101 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5341 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5401 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5461 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5521 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5581 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5641 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5701 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5761 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5821 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5881 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5941 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6001 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6061 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6121 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6181 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6241 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6301 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6361 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6421 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6481 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6541 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6601 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6661 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6721 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6781 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6841 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6901 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6961 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7021 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7081 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7141 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7201 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7261 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7321 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7381 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7441 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7501 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7561 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7621 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7681 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7741 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7801 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7861 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7921 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7981 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8041 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8101 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8161 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     8221 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8281 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8341 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8401 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8461 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8521 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8581 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8641 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8701 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8761 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8821 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8881 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8941 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9001 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9061 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9121 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9181 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9241 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9301 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9361 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9421 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9481 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9541 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9601 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9661 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9721 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9781 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9841 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9901 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9961 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
    10021 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
    10081 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
    10141 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    10201 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
    10261 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    10321 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    10381 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    10441 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    10501 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    10561 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10621 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10681 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10741 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10801 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    10861 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
