LOCUS       sequence-353755-       13114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0950
FEATURES             Location/Qualifiers
     source          1..13114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6052..6640
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6105..6125)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6523..6534)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6712..6945
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7023..7144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7060..7079)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7114..7133
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7184..7319
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7339..7358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7340..7358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7368..7385)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7368..7384)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7377..7399)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7526..7981
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7613..7632)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7823..7844
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8007..8111
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8112..8180,8181..8972)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8330..8349)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9143..9731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9632..9651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9885..9902
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10019..10040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10055..10060,10061..10078,10079..10085)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10093..10109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10098..10120
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10117..10133
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10117..10133
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10152..10172
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10154..10172
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10200..10426
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10427..10607
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10654..10779
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11272..11505
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11690..11734
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11965..12175
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11987..12183
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12034..12169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12215..12817
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12215..12234)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12711..12731
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12860..12977
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 ttccaagaga agaagagcta cctccccttc cagcagtgtc agcggggact ttgatgatgg
     1261 gcaccattct gtgtcaacac caggcccaag caggaaaagg aggagacttt ccaatcttcc
     1321 aactgtagat cctattgccg tgtgccatga actctataat accatccgag actataagga
     1381 tgaacagggc agacttctct gtgagctctt cattagggca ccaaagcgaa gaaatcaacc
     1441 agactattat gaagtggttt ctcagcccat tgacttgatg aaaatccaac agaaactaaa
     1501 aatggaagag tatgatgatg ttaatttgct gactgctgac ttccagcttc tttttaacaa
     1561 tgcaaagtcc tattataagc cagattctcc tgaatataaa gccgcttgca aactctggga
     1621 tttgtacctt cgaacaagaa atgagtttgt tcagaaagga gaagcagatg acgaagatga
     1681 tgatgaagat gggcaagaca atcagggcac agtgactgaa ggatcttctc cagcttactt
     1741 gaaggagatc ctggagcagc ttcttgaagc catagttgta gctacaaatc catcaggacg
     1801 tctcattagc gaactttttc agaaactgcc ttctaaagtg caatatccag attattatgc
     1861 aataattaag gagcctatag atctcaagac cattgcccag aggatacaga atggaagcta
     1921 caaaagtatt catgcaatgg ccaaagatat agatctcctc gcaaaaaatg ccaaaactta
     1981 taatgagcct ggctctcaag tattcaagga tgcaaattca attaaaaaaa tattttatat
     2041 gaaaaaggct gaaattgaac atcatgaaat ggctaagtca agtcttcgaa tgaggactcc
     2101 atccaacttg gctgcagcca gactgacagg tccttcacac agtaaaggca gccttggtga
     2161 agagagaaat cccactagca agtattaccg taataaaaga gcagtacaag gaggtcgttt
     2221 atcagcaatt acaatggcac ttcaatatgg ctcagaaagt gaagaagatg ctgctttagc
     2281 tgctgcacgc tatgaagagg gagagtcaga agcagaaagc atcacttcct ttatggatgt
     2341 ttcaaatcct ttttatcagc tttatgacac agttaggagt tgtcggaata accaagggca
     2401 gctaatagct gaaccttttt accatttgcc ttcaaagaaa aaataccctg attattacca
     2461 gcaaattaaa atgcccatat cactacaaca gatccgaaca aaactgaaga atcaagaata
     2521 tgaaacttta gatcatttgg agtgtgatct gaatttaatg tttgaaaatg ccaaacgcta
     2581 taatgtgccc aattcagcca tctacaagcg agttctaaaa ttgcagcaag ttatgcaggc
     2641 aaagaagaaa gagcttgcca ggagagacga tatcgaggac ggagacagca tgatctcttc
     2701 agccacctct gatactggta gtgccaaaag aaaaagtaaa aagaacataa gaaagcagcg
     2761 aatgaaaatc ttattcaatg ttgttcttga agctcgagag ccaggttcag gcagaagact
     2821 ttgtgaccta tttatggtta aaccatccaa aaaggactat cctgattatt ataaaatcat
     2881 cttggagcca atggacttga aaataattga gcataacatc cgcaatgaca aatatgctgg
     2941 tgaagaggga atgatagaag acatgaagct gatgttccgg aatgccaggc actataatga
     3001 ggagggctcc caggtttata atgatgcaca tatcctggag aagttactca aggagaaaag
     3061 gaaagagctg ggcccactgc ctgatgatga tgacatggct tctcccaaac tcaagctgag
     3121 taggaagagt ggcatttctc ctaaaaaatc aaaatacatg actccaatgc agcagaaact
     3181 aaatgaggtc tatgaagctg taaagaacta tactgataag aggggtcgcc gcctcagtgc
     3241 catatttctg aggcttccct ctagatctga gttgcctgac tactatctga ctattaaaaa
     3301 gcccatggac atggaaaaaa ttcgaagtca catgatggcc aacaagtacc aagatattga
     3361 ctctatggtt gaggactttg tcatgatgtt taataatgcc tgtacataca atgagccgga
     3421 gtctttgatc tacaaagatg ctcttgttct acacaaagtc ctgcttgaaa cacgcagaga
     3481 cctggaggga gatgaggact ctcatgtccc aaatgtgact ttgctgattc aagagcttat
     3541 ccacaatctt tttgtgtcag tcatgagtca tcaggatgat gagggaagat gctacagcga
     3601 ttctttagca gaaattcctg ctgtggatcc caactttcct aacaaaccac cccttacatt
     3661 tgacataatt aggaagaatg ttgaaaataa tcgctaccgt cggcttgatt tatttcaaga
     3721 gcatatgttt gaagtattgg aacgagcaag aaggatgaat cggacagatt cagaaatata
     3781 tgaagatgca gtagaacttc agcagttttt tattaaaatt cgtgatgaac tctgcaaaaa
     3841 tggagagatt cttctttcac cggcactcag ctataccaca aaacatttgc ataatgatgt
     3901 ggagaaagag agaaaggaaa aattgccaaa agaaatagag gaagataaac taaaacgaga
     3961 agaagaaaaa agagaagctg aaaagagtga agattcctct ggtgctgcag gcctctcagg
     4021 cttacatcgc acatacagcc aggactgtag ctttaaaaac agcatgtacc atgttggaga
     4081 ttacgtctat gtggaacctg cagaggccaa cctacaacca catatcgtct gtattgaaag
     4141 actgtgggag gattcagctg gtgaaaaatg gttgtatggc tgttggtttt accgaccaaa
     4201 tgaaacattc cacctggcta cacgaaaatt tctagaaaaa gaagttttta agagtgacta
     4261 ttacaacaaa gttccagtta gtaaaattct aggcaagtgt gtggtcatgt ttgtcaagga
     4321 atactttaag ttatgcccag aaaacttccg agatgaggat gtttttgtct gtgaatcacg
     4381 gtattctgcc aaaaccaaat cttttaagaa aattaaactg tggaccatgc ccatcagctc
     4441 agtcaggttt gtccctcggg atgtgcctct gcctgtggtt cgcgtggcct ctgtatttgc
     4501 aaatgcagat aaaggtgatg atgagaagaa tacagacaac tcagaggaca gtcgagctga
     4561 agacaatttt aacttggaaa aggaaaaaga agatgtccct gtggaaatgt ccaatggtga
     4621 accaggttgc cactactttg agcagctcca ttacaatgac atgtggctga aggttggcga
     4681 ctgtgtcttc atcaagtccc atggcctggt gcgtcctcgt gtgggcagaa ttgaaaaagt
     4741 atgggttcga gatggagctg catattttta tggccccatc ttcattcacc cagaagaaac
     4801 agagcatgag cccacaaaaa tgttctacaa aaaagaagta tttctgagta atctggaaga
     4861 aacctgcccc atgacatgta ttctcggaaa gtgtgctgtg ttgtcattca aggacttcct
     4921 ctcctgcagg ccaactgaaa taccagaaaa tgacattctg ctttgtgaga gccgctacaa
     4981 tgagagcgac aagcagatga agaaattcaa aggattgaag aggttttcac tctctgctaa
     5041 agtggtagat gatgaaattt actacttcag aaaaccaatt gttcctcaga aggagccatc
     5101 acctttgctg gaaaagaaga tccagttgct agaagctaaa tttgccgagt tagaaggtgg
     5161 agatgatgat attgaagaga tgggagaaga agatagtgag gtcattgaac ctccttctct
     5221 acctcagctt cagacccccc tggccagtga gctggacctc atgccctaca cacccccaca
     5281 gtctacccca aagtctgcca aaggcagtgc aaagaaggaa ggctccaaac ggaaaatcaa
     5341 catgagtggc tacatcctgt tcagcagtga gatgagggct gtgattaagg cccaacaccc
     5401 agactactct ttcggggagc tcagccgcct ggtggggaca gaatggagaa atcttgagac
     5461 agccaagaaa gcagaatatg aaggtgtgat gaaccaagga gtggccccta tggtagggac
     5521 tccagcacca ggtggaagtc catatggaca acaggtggga gttttggggc ctccagggca
     5581 gcaggcacca cctccatatc ccggcccaca tccagctgga ccccctgtca tacagcagcc
     5641 aacaacaccc atgtttgtag ctcccccacc aaagacccag cggcttcttc actcagaggc
     5701 ctacctgaaa tacattgaag gactcagtgc ggagtccaac agcattagca agtgggatca
     5761 gacactggca gctcgaagac gcgacgtcca tttgtcgaaa gaacaggaga gccgcctacc
     5821 ctctcactgg ctgaaaagca aaggggccca caccaccatg gcagatgccc tctggcgcct
     5881 tcgagatttg atgctccggg acaccctcaa cattcgccaa gcatacaacc tagaaaatgt
     5941 ttagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     6001 tggaaaggac gagtgagtcg gcgtcccgtc ctgcgaacgc gttaagtcga caatcaacct
     6061 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6121 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6181 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6241 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6301 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6361 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6421 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6481 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6541 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6601 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     6661 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6721 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     6961 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7021 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7081 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7141 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7201 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7261 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7321 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     7381 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7441 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7501 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7561 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7621 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7681 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7741 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7801 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7861 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7921 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7981 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8041 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8101 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8161 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8221 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8281 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8341 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8401 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8461 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8521 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8581 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8641 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8701 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8761 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8821 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8881 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8941 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9001 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9061 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9121 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9181 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9241 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9301 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9361 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9421 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9481 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9541 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9601 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9661 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9721 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9781 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9841 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9901 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9961 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10021 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10081 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    10141 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
    10201 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
    10261 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
    10321 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
    10381 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
    10441 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10501 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10561 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    10621 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    10681 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    10741 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    10801 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    10861 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    10921 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10981 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    11041 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    11101 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    11161 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11221 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11281 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11341 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11401 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11461 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11521 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11581 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11641 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11701 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11761 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11821 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11881 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
    11941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
    12001 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    12061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    12121 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    12181 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
    12241 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
    12301 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
    12361 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    12421 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    12481 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    12541 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    12601 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    12661 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    12721 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    12781 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
    12841 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    12901 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    12961 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    13021 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
    13081 atggaccttc taggtcttga aaggagtggg aatt
//
