LOCUS       sequence-353759-       10852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1708
FEATURES             Location/Qualifiers
     source          1..10852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3790..4378
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3843..3863)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4261..4272)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4450..4683
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4761..4882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4798..4817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4852..4871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4922..5057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5077..5096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5078..5096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5106..5123)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5106..5122)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5115..5137)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5264..5719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5351..5370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5561..5582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5745..5849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5850..5918,5919..6710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6068..6087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6881..7469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7370..7389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7623..7640
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7757..7778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7793..7798,7799..7816,7817..7823)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7831..7847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7836..7858
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7855..7871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7855..7871
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7890..7910
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7892..7910
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7938..8164
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8165..8345
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8392..8517
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9010..9243
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9428..9472
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9703..9913
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9725..9921
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9772..9907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9953..10555
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9953..9972)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10449..10469
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10598..10715
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 cagcgaggca ttccagagcg cgcgggactt tctggacatg aacttccagt cgctggccat
     1261 gaaacacatg gatctgaagc agatggagct ggacacggcg gcggccaagg tggatgaact
     1321 gaccaagcag ctggagtcgc tgtggtcaga ctctcccgcg cctcctggcc cgcaggccgg
     1381 acccccttct aggccgcccc ggtacagctc cagctcgatc cctgagccct tcggcagccg
     1441 agggtccccc cggaaggcgg ccaccgacgg cgcagacacc ccgttcggac gatcagagag
     1501 tgccccaacc ctacacccct acagcccgct gtcccccaag ggacggccgt cgtcgccgcg
     1561 caccccgctc tacctgcagc cggacgccta cggcagcctg gaccgcgcga cctcgccccg
     1621 gccccgcgcc ttcgatggcg caggcagctc cctcggccgt gcgccctccc cgcggcccgg
     1681 gccaggcccg ctccgccagc agggtccccc cacgcctttc gacttcctgg gccgcgcagg
     1741 ctccccccgc ggcagccccc tggcggaggg gccccaggcc ttcttccccg agcgtgggcc
     1801 gtcaccgcgc ccccctgcca cagcctacga cgcgccagcg tccgccttcg ggagctccct
     1861 gctaggctcc ggcggcagcg cattcgcccc gcctctgcgc gcgcaagacg acctgacgct
     1921 gcgccggcgg cctccgaaag cctggaacga gtctgacctg gacgtggcgt acgagaagaa
     1981 gccttcgcag acagcgagct atgaacgcct ggacgtcttc gcaaggcctg cctcgccgag
     2041 cctgcagctg ttgccttgga gggagagcag cctggatgga ctggggggca ccggcaagga
     2101 caacctcact agcgccaccc tgccgcgcaa ttacaaggtc tctcctctgg ccagcgaccg
     2161 gcgttcagac gcgggcagct accggcgctc gctgggctcc gcggggccgt cgggcacttt
     2221 gcctcgcagc tggcagcccg tcagccgcat ccccatgccc ccctccagcc cccagccccg
     2281 cggggccccg cgccagcgtc ccatccccct cagcatgatc ttcaagctgc agaacgcctt
     2341 ctgggagcac ggggccagcc gcgccatgct ccctgggtcc cccctcttca cccgagcacc
     2401 cccgcctaag ctgcagcccc aaccacaacc acagccccag ccacaatcac aaccacagcc
     2461 ccagctgccc ccacagcccc agacccaacc ccaaacccct accccagccc cccagcatcc
     2521 ccaacagaca tggccccctg tgaacgaagg accccccaaa ccccccaccg agctggagcc
     2581 tgagccggag atagaggggc tgctgacacc agtgctggag gctggcgatg tggatgaagg
     2641 ccctgtagca aggcctctca gccccacgag gctgcagcca gcactgccac cggaggcaca
     2701 gtcggtgccc gagctggagg aggtggcacg ggtgttggcg gaaattcccc ggcccctcaa
     2761 acgcaggggc tccatggagc aggcccctgc tgtggccctg ccccctaccc acaagaaaca
     2821 gtaccagcag atcatcagcc gcctcttcca tcgtcatggg gggccagggc ccggggggcc
     2881 ggagccagag ctgtccccca tcactgaggg atctgaggcc agggcagggc cccctgctcc
     2941 tgccccacca gctcccattc cacccccggc cccgtcccag agcagcccac cagagcagcc
     3001 gcagagcatg gagatgcgct ctgtgctgcg gaaggcgggc tccccgcgca aggcccgccg
     3061 cgcgcgcctc aaccctctgg tgctcctcct ggacgcggcg ctgaccgggg agctggaggt
     3121 ggtgcagcag gcggtgaagg agatgaacga cccgagccag cccaacgagg agggcatcac
     3181 tgccttgcac aacgccatct gcggcgccaa ctactctatc gtggatttcc tcatcaccgc
     3241 gggtgccaat gtcaactccc ccgacagcca cggctggaca cccttgcact gcgcggcgtc
     3301 gtgcaacgac acagtcatct gcatggcgct ggtgcagcac ggcgctgcaa tcttcgccac
     3361 cacgctcagc gacggcgcca ccgccttcga gaagtgcgac ccttaccgcg agggttatgc
     3421 tgactgcgcc acctacctgg cagacgtcga gcagagtatg gggctgatga acagcggggc
     3481 agtgtacgct ctctgggact acagcgccga gttcggggac gagctgtcct tccgcgaggg
     3541 cgagtcggtc accgtgctgc ggagggacgg gccggaggag accgactggt ggtgggccgc
     3601 gctgcacggc caggagggct acgtgccgcg gaactacttc gggctgttcc ccagggtgaa
     3661 gcctcaaagg agtaaagtct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     3721 ttggctttat atatcttgtg gaaaggacga agaaacatgc tcggtgctac ggttacgcgt
     3781 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3841 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3901 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3961 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4021 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4081 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4141 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4201 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4261 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4321 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     4381 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4441 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4681 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4741 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4801 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4861 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4981 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5041 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     5101 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5161 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5221 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5281 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5341 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5401 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5461 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5521 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5581 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5641 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5701 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5761 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5821 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5881 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5941 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6001 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6061 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6121 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6181 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6241 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6301 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6361 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6421 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6481 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6541 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6601 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6661 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6721 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6781 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6841 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6901 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6961 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7021 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7081 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7141 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7201 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7261 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7321 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7381 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7441 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7501 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7561 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7621 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7681 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7741 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7801 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7861 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7921 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7981 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     8041 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     8101 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     8161 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8221 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8281 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8341 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8401 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8461 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8521 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8581 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8641 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8701 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8761 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8821 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8881 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8941 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9001 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9061 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9121 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9181 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9241 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9301 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9361 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9421 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9481 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9541 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9601 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     9661 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9721 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9901 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     9961 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    10021 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    10081 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    10141 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
    10201 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    10261 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    10321 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    10381 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    10441 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    10501 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
    10561 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    10621 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    10681 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    10741 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
    10801 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
