LOCUS       sequence-353760-       10762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0072
FEATURES             Location/Qualifiers
     source          1..10762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3700..4288
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3753..3773)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4171..4182)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4360..4593
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4671..4792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4708..4727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4762..4781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4832..4967
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4987..5006)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4988..5006)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5016..5033)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5016..5032)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5025..5047)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5174..5629
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5261..5280)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5471..5492
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5655..5759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5760..5828,5829..6620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5978..5997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6791..7379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7280..7299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7533..7550
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7667..7688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7703..7708,7709..7726,7727..7733)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7741..7757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7746..7768
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7765..7781
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7765..7781
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7800..7820
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7802..7820
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7848..8074
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8075..8255
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8302..8427
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8920..9153
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9338..9382
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9613..9823
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9635..9831
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9682..9817
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9863..10465
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9863..9882)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10359..10379
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10508..10625
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgag
     1201 ctttgatcca aaccttctcc acaacaatgg acataatggg taccctaatg gtacttcagc
     1261 agcactgcgt gaaactgggg ttattgaaaa actgttaacc tcttacggat ttattcagtg
     1321 ttcagaacgt caagctagac ttttcttcca ctgttcacag tataatggca acctgcaaga
     1381 cttaaaagta ggagatgatg ttgaatttga agtatcatcg gaccgacgga ctgggaaacc
     1441 cattgctgtt aaactggtga agataaaaca agaaatcctc cctgaagaac gaatgaatgg
     1501 acaagttgtg tgcgctgttc ctcacaactt agagagtaaa tctccagctg ccccgggtca
     1561 gagtccaaca gggagtgtat gctacgaacg taatggggaa gtgttttatc tgacttacac
     1621 ccctgaagat gtcgaaggga acgttcagct ggaaactgga gataaaataa actttgtaat
     1681 tgataacaat aaacatactg gtgctgtaag tgctcgcaac attatgctgt tgaaaaagaa
     1741 acaagcccgc tgtcagggag tagtttgtgc catgaaggag gcatttggct ttattgaaag
     1801 aggtgatgtt gtaaaagaga tattctttca ctatagtgaa tttaagggtg acttagaaac
     1861 cttacagcct ggcgatgatg tggaattcac aatcaaggac agaaatggta aagaagttgc
     1921 aacagatgtc agactattgc ctcaaggaac agtcattttt gaagatatca gcattgaaca
     1981 ttttgaagga actgtaacca aagttatccc aaaagtaccc agtaaaaacc agaatgaccc
     2041 attgccagga cgcatcaaag ttgactttgt gatccctaaa gaacttccct ttggagacaa
     2101 agatacgaaa tccaaggtga ccctgctgga aggtgaccat gttaggttta atatttcaac
     2161 agaccgacgt gacaaattag agcgagcaac caatatagaa gttctgtcaa atacatttca
     2221 gttcactaat gaagcccgag aaatgggtgt gattgctgcc atgagagatg gttttggttt
     2281 catcaagtgt gtggatcgtg atgttcgtat gttcttccac ttcagtgaaa ttctggatgg
     2341 gaaccagctc catattgcag atgaagtaga gtttactgtg gttcctgata tgctctctgc
     2401 tcaaagaaat catgctatta ggattaaaaa acttcccaag ggcacggttt catttcattc
     2461 ccattcagat caccgttttc tgggcacggt agaaaaagaa gccacttttt ccaatcctaa
     2521 aaccactagc ccaaataaag gcaaagagaa ggaggctgag gatggcatta ttgcttatga
     2581 tgactgtggg gtgaaactga ctattgcttt tcaagccaag gatgtggaag gatctacttc
     2641 tcctcaaata ggagataagg ttgaatttag tattagtgac aaacagaggc ctggacagca
     2701 ggttgcaact tgtgtgcgac ttttaggtcg taattctaac tccaagaggc tcttgggtta
     2761 tgtggcaact ctgaaggata attttggatt tattgaaaca gccaatcatg ataaggaaat
     2821 ctttttccat tacagtgagt tctctggtga tgttgatagc ctggaactgg gggacatggt
     2881 cgagtatagc ttgtccaaag gcaaaggcaa caaagtcagt gcagaaaaag tgaacaaaac
     2941 acactcagtg aatggcatta ctgaggaagc tgatcccacc atttactctg gcaaagtaat
     3001 tcgccccctg aggagtgttg atccaacaca gactgagtac caaggaatga ttgagattgt
     3061 ggaggagggc gatatgaaag gtgaggtcta tccatttggc atcgttggga tggccaacaa
     3121 aggggattgc ctgcagaaag gggagagcgt caagttccaa ttgtgtgtcc tgggccaaaa
     3181 tgcacaaact atggcttaca acatcacacc cctgcgcagg gccacagtgg aatgtgtgaa
     3241 agatcagttt ggcttcatta actatgaagt aggagatagc aagaagctct ttttccatgt
     3301 gaaagaagtt caggatggca ttgagctaca ggcaggagat gaggtggagt tctcagtgat
     3361 tcttaatcag cgcactggca agtgcagcgc ctgtaatgtt tggcgagtct gtgagggccc
     3421 caaggctgtt gcagctcctc gacctgatcg gttggtcaat cgcttgaaga atatcactct
     3481 ggatgatgcc agtgctcctc gcctaatggt tcttcgtcag ccaaggggac cagataactc
     3541 aatggggttt ggtgcagaaa gaaagatccg tcaagctggt gtcattgact agtaatgaac
     3601 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     3661 agctataaat acgtgtataa acccacgcgt taagtcgaca atcaacctct ggattacaaa
     3721 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3781 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3841 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3901 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3961 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4021 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4081 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4141 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4201 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4261 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     4321 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4381 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4441 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4501 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4561 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     4621 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4681 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4741 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4801 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4861 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4921 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     4981 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     5041 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5101 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5161 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5221 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5281 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5341 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5401 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5461 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5521 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5581 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     5641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7561 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7621 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7681 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7741 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     7801 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     7861 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7921 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     7981 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8041 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8101 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8161 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8221 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8281 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8341 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     8401 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     8461 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8521 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     8581 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     8641 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     8701 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     8761 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8821 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8881 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8941 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9001 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9061 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9121 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9181 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9241 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9301 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9361 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9421 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9481 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9541 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     9601 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     9661 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9721 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9781 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     9841 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9901 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9961 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
    10021 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
    10081 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    10141 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    10201 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    10261 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    10321 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    10381 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    10441 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
    10501 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    10561 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    10621 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    10681 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    10741 ggtcttgaaa ggagtgggaa tt
//
