LOCUS       sequence-353763-        9874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2675
FEATURES             Location/Qualifiers
     source          1..9874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2812..3400
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2865..2885)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3283..3294)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3472..3705
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3783..3904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3820..3839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3874..3893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3944..4079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4099..4118)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4100..4118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4128..4145)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4128..4144)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4137..4159)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4286..4741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4373..4392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4583..4604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4767..4871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4872..4940,4941..5732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5090..5109)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5903..6491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6392..6411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6645..6662
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6779..6800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6815..6820,6821..6838,6839..6845)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6853..6869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6858..6880
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6877..6893
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6877..6893
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6912..6932
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6914..6932
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6960..7186
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7187..7367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7414..7539
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8032..8265
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8450..8494
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8725..8935
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8747..8943
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8794..8929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8975..9577
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8975..8994)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9471..9491
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9620..9737
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 aactcttcag gaatccttaa acacattact taaacagcta gaagaagaaa agaagactct
     1261 tgaaagtcaa gtgaaatact atgcacttaa actggaacaa gaatcaaagg cttaccagaa
     1321 gatcaacaat gaacgccgta catacctagc tgaaatgtct cagggttctg gtttacatca
     1381 agtttctaaa aggcaacagg tggatcaact gcctaggatg caagagaatc tagtgaaaac
     1441 cctcctctta aaagaagaat tggacccttt aaaggcaaaa atagacatct tattagttgg
     1501 agatgtcact gtgggctacc tggctgatac tgtacagaaa ctatttgcaa acatagcaga
     1561 agtcaccatc accatcagtg acacgaagga ggcagcagcg cttttggatg attgcatatt
     1621 caacatggtt ctcttgaagg tgccttcttc actaagtgcc gaggagctgg aagccatcaa
     1681 gttaattaga tttggcaaaa agaaaaatac acattcactg tttgttttta taatccctga
     1741 aaattttaaa ggttgtattt cagggcatgg aatggatatt gctttaactg aaccactgac
     1801 aatggaaaaa atgagtaatg tggtaaaata ctggacaaca tgtccctcaa acactgttaa
     1861 gactgaaaac gcaactgggc ctgaagaact tggattgccc ctgcagaggt cctacagcga
     1921 acacctggga tattttccta ctgatctatt tgcctgctct gaatctttaa ggaatggcaa
     1981 tgggcttgaa ttaaatgctt cgttgtcaga gttcgagaaa aacaaaaaga tctctcttct
     2041 tcattcaagc aaggaaaaac taagaaggga aagaatcaaa tattgctgtg agcagctgcg
     2101 tactctcttg ccgtatgtaa aagggagaaa gaatgatgcg gcttcagttc ttgaggcaac
     2161 agttgattat gtgaaatata tccgggagaa aatctctcca gccgttatgg cccagattac
     2221 agaagcactt cagagcaaca tgaggttttg taagaaacaa caaacaccca ttgagctgtc
     2281 tctcccaggc actgtcatgg cacagcggga aaacagtgtg atgagcactt actcccctga
     2341 gagagggctc caattcctga ctaatacgtg ctggaatggg tgctccactc ctgatgcaga
     2401 gagctccttg gatgaagctg tgagagttcc atcaagctcc gcctcagaga atgctattgg
     2461 tgatccatat aaaactcaca tttccagtgc agcgctgtct ctgaattcct tgcatactgt
     2521 cagatattat tctaaagtca ccccttccta cgatgcaact gctgtaacaa atcagaacat
     2581 ttcaattcat ttaccttcag ccatgccccc ggtctcaaag cttctccctc ggcactgcac
     2641 ttctgggttg ggccagacgt gcactacaca tcccaactgt ctgcaacagt tttgggcgta
     2701 ttagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2761 tggaaaggac gacaaacggc gcagattcaa tctatcacgc gttaagtcga caatcaacct
     2821 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2881 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2941 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3001 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3061 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3121 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3181 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3241 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3301 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3361 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     3421 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3481 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3541 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3601 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3661 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     3721 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     3781 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3841 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3901 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     3961 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4021 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4081 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4141 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4201 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4261 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4321 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4381 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4441 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4501 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4561 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4621 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4681 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4741 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4861 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4921 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4981 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5041 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5101 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5161 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5221 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5281 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5341 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5401 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5461 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5521 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5581 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5641 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5701 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5761 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5941 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6001 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6061 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6121 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6181 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6241 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6301 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6361 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6421 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6481 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6541 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6601 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6661 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6721 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6781 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6841 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6901 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     6961 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7021 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7081 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7141 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7381 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7441 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7501 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7561 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7621 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7681 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7741 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7801 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7861 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     7921 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7981 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8041 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8101 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8161 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8221 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8281 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8341 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8401 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8461 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8521 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8581 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8641 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     8701 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     8761 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8821 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8881 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8941 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     9001 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9061 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9121 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9181 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9241 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     9301 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9361 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9421 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9481 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9541 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
     9601 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9661 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9721 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
     9781 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     9841 atggaccttc taggtcttga aaggagtggg aatt
//
