LOCUS       sequence-353764-       11407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0067
FEATURES             Location/Qualifiers
     source          1..11407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4345..4933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4398..4418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4816..4827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5005..5238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5316..5437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5353..5372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5407..5426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5477..5612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5632..5651)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5633..5651)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5661..5678)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5661..5677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5670..5692)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5819..6274
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5906..5925)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6116..6137
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6300..6404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6405..6473,6474..7265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6623..6642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7436..8024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7925..7944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8178..8195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8312..8333
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8348..8353,8354..8371,8372..8378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8386..8402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8391..8413
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8410..8426
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8410..8426
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8445..8465
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8447..8465
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8493..8719
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8720..8900
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8947..9072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9565..9798
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9983..10027
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10258..10468
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10280..10476
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10327..10462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10508..11110
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10508..10527)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11004..11024
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11153..11270
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 agacccgggg tcggagataa tagaatctgt ccctccagct ggccctgagg catctgagtc
     1261 aacaacggat gaaaatgaag acgacattca gtttgtcagt gaaggaccat tacgacctgt
     1321 tcttgaatac attgatctgg tcagcagtga tgatgaagag cctagcacct cttatactga
     1381 tgagaatatt aaacgtaaag accatattga ttatcagaag gataaagttg ctttaactct
     1441 ggctcgtcta gcccgccatg ttgaagtgga gaaacagcag aaagaagaga agaatagagc
     1501 attcagagaa aaaattgatt ttcagcatgc tcatgggtta caagaattgg aatttattcg
     1561 aggacattct gatacagaag cagcaagact gtgtgtggac cagtggctaa aaatgccagg
     1621 actcaaaaca ggcacaatta attgtggaac aaaaagttca ttccgaagag gaggccacac
     1681 gtgggtgtct gggaaaccaa ttttatgtcc tataatgcac tgtaacaagg agtttgacaa
     1741 tgggcacctt ctcttaggac atttgaaaag gttcgatcac tctccatgtg atccaacaat
     1801 tacactacat ggacctttct tcagctcctt tgcttgtgta gtatgttata aaaaatttgt
     1861 tactcaacaa caatatagag atcacctttt tgataaggaa gccacagatg atggacataa
     1921 caacaacctt cttcctcaga ttattcagtg ttttgcatgt ccaaattgct tccttctttt
     1981 tagcagaaag gaggagtgtt caaagcatat gtctggaaag aatcatttcc atcagagttt
     2041 caaactgggt gataacaaag gaattgcaca tccaatatct ttcccatctt ttgcaaagaa
     2101 acttttgatc tctctgtgca aagatgttcc ctttcaagtt aagtgtgtgg cctgccacaa
     2161 gacactgcgt tcccacatgg agctcactgc ccatttcaga gttcattgtc gaaatgctgg
     2221 acctgtagct gtagctgaga agagcattac ccaggttgca gagaaattca tattaagagg
     2281 ttattgtcca gattgcaatc aagtctttgt ggatgaaacc agcacccaaa atcataagca
     2341 gaattcagga cacaaagtcc gagtcattaa ctcagtggaa gaatcagtct tactctattg
     2401 ccacagcagc gaagggaaca aggatccttc ttctgacttg catttattgt tggatcaatc
     2461 aaaattttca tcacttaaaa gaaccatgtc tattaaagaa tctagctcac tggagtgcat
     2521 tgccattcca aaaaagaaga tgaatttaaa agataaaagc catgaaggtg ttgcttgtgt
     2581 ccagaaagaa aaatcagtag ttaaaacctg gttctgtgaa tgcaatcagc gattcccaag
     2641 tgaagatgca gtagaaaagc atgttttctc agcaaacaca atgggttata aatgtgtggt
     2701 ctgtggaaag gtatgtgatg attcaggggt cattcgttta cacatgagcc ggattcacgg
     2761 aggggcacat ttaaataact ttcttttctg gtgtcggaca tgcaaaaagg agttaacaag
     2821 gaaagatact atcatggcac atgtgactga atttcataat ggacacagat atttttatga
     2881 gatggatgag gtagaaggtg aaactttgcc atcatcctct acaacattgg ataatttgac
     2941 tgctaacaag ccttcatcag ctattactgt tattgatcat tccccggcaa atagttctcc
     3001 gaggggtaaa tggcaatgcc ggatttgtga agatatgttt gattcccagg aatatgtaaa
     3061 acagcactgc atgtctttgg caagccacaa gtttcataga tacagctgtg ctcactgcag
     3121 aaagcctttt cataagatag aaacattgta ccgacattgc caagatgagc atgacaatga
     3181 gataaagatt aaatacttct gtgggctttg tgatcttatc tttaatgtgg aagaagcatt
     3241 tctgagtcat tatgaggagc accacagcat agattatgta tttgtgtcag aaaaaactga
     3301 aacttcaatt aaaaccgaag atgattttcc agtaatagag accagtaacc agttaacttg
     3361 tggttgccgt gagagttaca tctgtaaagt caacagaaaa gaagattata gcagatgtct
     3421 ccaaatcatg ctggataaag gaaaactgtg gtttcgctgc agtttatgtt cggcaacagc
     3481 acagaattta accgacatga acactcatat ccatcaagtg cacaaagaaa agagtgatga
     3541 ggaggagcag cagtatgtaa tcaagtgtgg cacctgcacc aaagcatttc atgatcctga
     3601 gagtgcacag cagcatttcc atagaaaaca ttgcttctta cagaaaccca gtgtggctca
     3661 ttttggatct gaaaaatcaa acctgtacaa gtttactgct agtgcctcac atacagagag
     3721 aaaactgaaa caggcaataa actattcaaa aagtttagac atggagaaag gagttgagaa
     3781 tgacctaagc tatcagaata taggaggaaa caccaattgg aagcctccgc tcaactgtaa
     3841 gatttataac tacctgaaca ggattggatg cttcttcctt catcctcgct gtagtaaaag
     3901 aaaagatgct gctgattttg ccatatgtat gcatgctggc cgtctagatg aacaactacc
     3961 caagcaaatt cctttcacca tcctctcagg agatcaaggt tttctggagc tagagaatca
     4021 atttaagaag actcagaggc cagctcatat actaaaccct caccacttag agggagatat
     4081 gatgtgtgcc ttgttaaata gcatatctga taccaccaaa gaatgtgaca gtgatgataa
     4141 catgggtgcc aaaaatactt caataggaga agaatttata tccacagaag atgtggaatt
     4201 agaagaagct attagaagaa gtcttgagga aatgtagtaa tgaactagtc cgtaacttga
     4261 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaagcc ggcccattaa
     4321 gcccaagtta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     4381 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4441 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4501 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4561 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4621 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4681 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4741 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4801 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4861 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4921 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4981 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5041 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5101 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5161 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5221 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     5281 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     5341 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5401 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     5461 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5521 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5581 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     5641 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5701 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5761 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     5821 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5881 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5941 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6001 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6061 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6121 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6181 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6241 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     6301 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6361 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6421 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6481 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6541 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6601 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6661 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6721 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6781 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6841 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6901 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6961 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7021 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7081 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7141 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7201 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7261 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7321 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7381 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7441 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7501 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7561 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7621 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7681 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7741 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7801 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7861 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7921 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7981 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8041 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8101 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8161 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8221 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8281 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8341 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8401 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     8461 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     8521 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     8581 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     8641 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     8701 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     8761 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8821 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8881 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8941 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9001 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9061 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9121 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9181 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9241 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9301 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9361 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     9421 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9481 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9541 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9601 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9661 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9721 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9781 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9841 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9901 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9961 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10021 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10081 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    10141 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    10201 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    10261 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
    10321 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10381 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10441 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
    10501 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
    10561 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
    10621 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
    10681 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
    10741 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
    10801 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
    10861 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
    10921 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
    10981 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
    11041 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
    11101 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
    11161 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    11221 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    11281 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
    11341 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
    11401 gggaatt
//
