LOCUS       sequence-353766-       15501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AMA1 based expression in filamentous fungi pYFAC-pyrG carrying 
            PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction 
            site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for 
            PaldA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15501)
  AUTHORS   Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, 
            Karton A, Piggott AM, Chooi YH
  TITLE     Heterologous biosynthesis of elsinochrome A sheds light on the 
            formation of the photosensitive perylenequinone system.
  JOURNAL   Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. 
            eCollection 2019 Feb 7.
  PUBMED    30809363
REFERENCE   2  (bases 1 to 15501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c8sc02870b"; journalName: "Chem Sci"; date: "2018-11-22- 
            22"; volume: "10"; issue: "5"; pages: "1457-1465"
COMMENT     Sequence Label: pYFAC-CH2
FEATURES             Location/Qualifiers
     source          1..15501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2947..3015,3016..3807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3165..3184)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3978..4566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4467..4486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5150..5183
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5262..6065)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6066..6286)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6457..6476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6498..11746
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     primer_bind     11916..11937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attaagccca gaaaattacg ggtatgtcct ccactgtgtt gaagtcagaa aaacaatcga
       61 taatttgaag ctcattaaaa tgccagttcc gtcgtcgccg ttgccctagg agctattcca
      121 gtgcttagct tcattcatgg atgtatcgtg actggccttc ggaaggatgc caaggtcccc
      181 tatccgcaca gctatgcgac cgttgagcaa tgcaagtcaa atgtgtgcat acctggtctt
      241 tggtttggta caccgctatg tgaagattat taatgctgaa gtcatgatac aggccaaagc
      301 cgagcaattt aactgcgcgc agcgggccca cgctaactac ctcgagaata tgcctcaaac
      361 aatgctcttc accctcttcg cgggtctgaa ataccctcac ctggcaacag caattggcgc
      421 ttcatggctg tttttccgat ctctctactt gtacggctat gtgtactcgg gtaagccaca
      481 aggcaagggc agattgctgg gaggtttctt ctggttttct caaggcgctc tgtgggctct
      541 gagtgtgttt ggtgttgcca aagacatgat ctcttactga gagttattct gtgtctgacg
      601 aaatatgttg tgtatatata tatatgtacg ttaaaagttc cgtggagtta ccagtgattg
      661 accaatgttt tatcttctac agttctgcct gtctacccca ttctagctgt acctgactac
      721 agagtagttt aattgtggtt gaccccacag tcggaggcgg aggaatacag caccgatgtg
      781 gcctgtctcc atccagattg gcacgcaatt tttacacgcg gaaaagatcg agatagagta
      841 cgactttaaa tttagtcccc ggcggcttct attttagaat atttgagatt tgattctcaa
      901 gcaattgatt tggttgggtc accctcaatt ggataatata cctcattgct cggctacttc
      961 aactcatcaa tcaccgtcat accccgcata taaccctcca ttcccacgat gtcgtccaag
     1021 tcgcaattga cttacggtgc tcgagccagc aagcacccca atcctctggc aaagagactt
     1081 tttgagattg ccgaagcaaa gaagacaaac gttaccgtct ctgctgatgt gacgacaacc
     1141 cgagaactcc tggacctcgc tgaccgtacg gaagctgttg gatccaatac atatgccgtc
     1201 tagcaatgga ctaatcaact tttgatgata caggtctcgg tccctacatc gccgtcatca
     1261 agacacacat cgacatcctc accgatttca gcgtcgacac tatcaatggc ctgaatgtgc
     1321 tggctcaaaa gcacaacttt ttgatcttcg aggaccgcaa attcatcgac atcggcaata
     1381 ccgtccagaa gcaataccac ggcggtgctc tgaggatctc cgaatgggcc cacattatca
     1441 actgcagcgt tctccctggc gagggcatcg tcgaggctct ggcccagacc gcatctgcgc
     1501 aagacttccc ctatggtcct gagagaggac tgttggtcct ggcagagatg acctccaaag
     1561 gatcgctggc tacgggcgag tataccaagg catcggttga ctacgctcgc aaatacaaga
     1621 acttcgttat gggtttcgtg tcgacgcggg ccctgacgga agtgcagtcg gatgtgtctt
     1681 cagcctcgga ggatgaagat ttcgtggtct tcacgacggg tgtgaacctc tcttccaaag
     1741 gagataagct tggacagcaa taccagactc ctgcatcggc tattggacgc ggtgccgact
     1801 ttatcatcgc cggtcgaggc atctacgctg ctcccgaccc ggttgaagct gcacagcggt
     1861 accagaaaga aggctgggaa gcttatatgg ccagagtatg cggcaagtca tgatttcctc
     1921 ttggagcaaa agtgtagtgc cagtacgagt gttgtggagg aaggctgcat acattgtgcc
     1981 tgtcattaaa cgatgagctc gtccgtattg gcccctgtaa tgccatgttt tccgccccca
     2041 atcgtcaagg ttttcccttt gttagattcc taccagtcat ctagcaagtg aggtaagctt
     2101 tgccagaaac gccaaggctt tatctatgta gtcgataagc aaagtggact gatagcttaa
     2161 tatggaaggt ccctcaggac aagtcgacct gtgcagaaga gataacagct tggcatcacg
     2221 catcagtgcc tcctctcaga cagaataagt cgccataagt tatcgaccga acctaggtag
     2281 ggtatatttg gtgacgacaa tacctcccga cgatacctgg attagacacc gtatgccgcg
     2341 aattaacgcc ttccgaccaa gtcccttttg gtgatcgaca ctttaggatc atctcctgcc
     2401 agtcgcccag actgacggtg ttatggtggc gcgctatggc tttcctagcc ggtcaagctc
     2461 cgtgcactag agagcggaga aggaggggga gttgataggg aatgatatct aggtagagaa
     2521 gtactccaac cagagatatg cggatcaatg ctccggcagt tgttagcata ccccaacctc
     2581 gtttaagtat cctcttcttc catccatctt tatcattatt tgtctatgtg catccctaga
     2641 tcacgtcaat tatttcgata aatggatacg tgcactcggg aatgcgtgat tggctcgtct
     2701 cgtgttctca gttcctcatt cccccccacg ttacattgac aacggagtat tagcctatgg
     2761 tcctgttcga taagcttgct ccatcgcctc cactacgtca agcggagtat cagacatgag
     2821 ctctacccca gatatcgcct gcttatcgct ggttgctttg tcatgcaagt acagatcaca
     2881 tcggcaattc tgataacaga tggtgccatt gtcccgtaat caattgccca ttgaaaaagg
     2941 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3001 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3061 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3121 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3181 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3241 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3301 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3361 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3421 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3481 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3541 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3601 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3661 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3721 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3781 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3841 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4621 catgattacg aattcatcac gtgctataaa aataattata atttaaattt tttaatataa
     4681 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     4741 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
     4801 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     4861 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     4921 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     4981 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
     5041 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
     5101 aaaagatacg aggcgcgtgt aagttacgaa ttcgagctcg gtacccggga taacttcgta
     5161 taatgtatgc tatacgaagt tatgggtaat aactgatata attaaattga agctctaatt
     5221 tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg ctggccgcat
     5281 cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac cttagcatcc
     5341 cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt agagaccaca
     5401 tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa acccacaccg
     5461 ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata
     5521 aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt agtatattct
     5581 ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc
     5641 tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg
     5701 tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga gtactgcaat
     5761 ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa attgtacttg
     5821 gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt caagatatcc
     5881 acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc cagtaattcc
     5941 ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg catgatatta
     6001 aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata tgtagctttc
     6061 gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt taagaatact
     6121 gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct aagtctgtgc
     6181 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac
     6241 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc gtggtgcagc
     6301 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     6361 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6421 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     6481 ttctcgccac gttcgccagc ttattttttg tatactgttt tgtgatagca cgaagttttt
     6541 ccacggtatc ttgttaaaaa tatatatttg tggcgggctt acctacatca aattaataag
     6601 agactaatta taaactaaac acacaagcaa gctactttag ggtaaaagtt tataaatgct
     6661 tttgacgtat aaacgttgct tgtatttatt attacaatta aaggtggata gaaaacctag
     6721 agactagtta gaaactaatc tcaggtttgc gttaaactaa atcagagccc gagaggttaa
     6781 cagaacctag aaggggacta gatatccggg tagggaaaca aaaaaaaaaa acaagacagc
     6841 cacatattag ggagactagt tagaagctag ttccaggact aggaaaataa aagacaatga
     6901 taccacagtc tagttgacaa ctagatagat tctagattga ggccaaagtc tctgagatcc
     6961 aggttagttg caactaatac tagttagtat ctagtctcct ataactctga agctagaata
     7021 acttactact attatcctca ccactgttca gctgcgcaaa cggagtgatt gcaaggtgtt
     7081 cagagactag ttattgacta gtcagtgact agcaataact aacaaggtat taacctacca
     7141 tgtctgccat caccctgcac ttcctcgggc tcagcagcct tttcctcctc attttcatgc
     7201 tcattttcct tgtttaagac tgtgactagt caaagactag tccagaacca caaaggagaa
     7261 atgtcttacc actttcttca ttgcttgtct cttttgcatt atccatgtct gcaactagtt
     7321 agagtctagt tagtgactag tccgacgagg acttgcttgt ctccggattg ttggaggaac
     7381 tctccagggc ctcaagatcc acaacagagc cttctagaag actggtcaat aactagttgg
     7441 tctttgtctg agtctgactt acgaggttgc atactcgctc cctttgcctc gtcaatcgat
     7501 gagaaaaagc gccaaaactc gcaatatggc tttgaaccac acggtgctga gactagttag
     7561 aatctagtcc caaactagct tggatagctt acctttgccc tttgcgttgc gacaggtctt
     7621 gcagggtatg gttcctttct caccagctga tttagctgcc ttgctaccct cacggcggat
     7681 ctgcataaag agtggctaga ggttataaat tagcactgat cctaggtacg gggctgaatg
     7741 taacttgcct ttcctttctc atcgcgcggc aagacaggct tgctcaaatt cctaccagtc
     7801 acaggggtat gcacggcgta cggaccactt gaactagtca cagattagtt agcaactagt
     7861 ctgcattgaa tggctgtact tacgggccct cgccattgtc ctgatcattt ccagcttcac
     7921 cctcgttgct gcaaagtagt tagtgactag tcaaggacta gttgaaatgg gagaagaaac
     7981 tcacgaattc tcgacaccct tagtattgtg gtccttggac ttggtgctgc tatatattag
     8041 ctaatacact agttagactc acagaaactt acgcagctcg cttgcgcttc ttggtaggag
     8101 tcggggttgg gagaacagtg ccttcaaaca agccttcata ccatgctact tgactagtca
     8161 gggactagtc accaagtaat ctagatagga cttgcctttg gcctccatca gttccttcat
     8221 agtgggaggt ccattgtgca atgtaaactc catgccgtgg gagttcttgt ccttcaagtg
     8281 cttgaccaat atgtttctgt tggcagaggg aacctgtcaa ctagttaata actagtcaga
     8341 aactagtata gcagtagact cactgtacgc ttgaggcatc ccttcactcg gcagtagact
     8401 tcatatggat ggatatcagg cacgccattg tcgtcctgtg gactagtcag taactaggct
     8461 taaagctagt cgggtcggct tactatcttg aaatccggca gcgtaagctc cccgtcctta
     8521 actgcctcga gatagtgaca gtactctggg gactttcgga gatcgttatc gcgaatgctc
     8581 ggcatactaa tcgttgacta gtcttggact agtcccgagc aaaaaggatt ggaggaggag
     8641 gaggaaggtg agagtgagac aaagagcgaa ataagagctt caaaggctat ctctaagcag
     8701 tatgaaggtt aagtatctag ttcttgacta gatttaaaag agatttcgac tagttatgta
     8761 cctggagttt ggatatagga atgtgttgtg gtaacgaaat gtaaggggga ggaaagaaaa
     8821 agtcggtcaa gaggtaactc taagtcggcc attccttttt gggaggcgct aaccataaac
     8881 ggcatggtcg acttagagtt agctcaggga atttagggag ttatctgcga ccactggact
     8941 ctcaggcttt ccattggggt actaacatag ccatcaaatg ccggcccgtg tttaatctcc
     9001 tcgagatggc tgggctcaca tgcgttctac tcaaaaactc ttatacctag gccctctccc
     9061 aatcgtgtat gtttggacct tcctctctca cactttggat aacatcaagc atactgcaga
     9121 ttttgaccta gttaacaagg cacatcatgc tatcacttcc aacactcttg ctgagcgcgt
     9181 tctgccttgc ctttctggtt ctacgaagta tccagtcccg cctgcaacgc cgcagaaacg
     9241 ccaaaaggtg gggttgtcaa ccgccagcta gagctccatc gggattcttt ggcattccgc
     9301 cgttcctcgg tggtcgcaga taactcccta aattccctga gctaactcta agtcgaccat
     9361 gccgtttatg gttagcgcct cccaaaaagg aatggccgac ttagagttac ctcttgaccg
     9421 actttttctt tcctccccct tacatttcgt taccacaaca cattcctata tccaaactcc
     9481 aggtacataa ctagtcgaaa tctcttttaa atctagtcaa gaactagata cttaaccttc
     9541 atactgctta gagatagcct ttgaagctct tatttcgctc tttgtctcac tctcaccttc
     9601 ctcctcctcc tccaatcctt tttgctcggg actagtccaa gactagtcaa cgattagtat
     9661 gccgagcatt cgcgataacg atctccgaaa gtccccagag tactgtcact atctcgaggc
     9721 agttaaggac ggggagctta cgctgccgga tttcaagata gtaagccgac ccgactagct
     9781 ttaagcctag ttactgacta gtccacagga cgacaatggc gtgcctgata tccatccata
     9841 tgaagtctac tgccgagtga agggatgcct caagcgtaca gtgagtctac tgctatacta
     9901 gtttctgact agttattaac tagttgacag gttccctctg ccaacagaaa catattggtc
     9961 aagcacttga aggacaagaa ctcccacggc atggagttta cattgcacaa tggacctccc
    10021 actatgaagg aactgatgga ggccaaaggc aagtcctatc tagattactt ggtgactagt
    10081 ccctgactag tcaagtagca tggtatgaag gcttgtttga aggcactgtt ctcccaaccc
    10141 cgactcctac caagaagcgc aagcgagctg cgtaagtttc tgtgagtcta actagtgtat
    10201 tagctaatat atagcagcac caagtccaag gaccacaata ctaagggtgt cgagaattcg
    10261 tgagtttctt ctcccatttc aactagtcct tgactagtca ctaactactt tgcagcaacg
    10321 agggtgaagc tggaaatgat caggacaatg gcgagggccc gtaagtacag ccattcaatg
    10381 cagactagtt gctaactaat ctgtgactag ttcaagtggt ccgtacgccg tgcatacccc
    10441 tgtgactggt aggaatttga gcaagcctgt cttgccgcgc gatgagaaag gaaaggcaag
    10501 ttacattcag ccccgtacct aggatcagtg ctaatttata acctctagcc actctttatg
    10561 cagatccgcc gtgagggtag caaggcagct aaatcagctg gtgagaaagg aaccataccc
    10621 tgcaagacct gtcgcaacgc aaagggcaaa ggtaagctat ccaagctagt ttgggactag
    10681 attctaacta gtctcagcac cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt
    10741 ctcatcgatt gacgaggcaa agggagcgag tatgcaacct cgtaagtcag actcagacaa
    10801 agaccaacta gttattgacc agtcttctag aaggctctgt tgtggatctt gaggccctgg
    10861 agagttcctc caacaatccg gagacaagca agtcctcgtc ggactagtca ctaactagac
    10921 tctaactagt tgcagacatg gataatgcaa aagagacaag caatgaagaa agtggtaaga
    10981 catttctcct ttgtggttct ggactagtct ttgactagtc acagtcttaa acaaggaaaa
    11041 tgagcatgaa aatgaggagg aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag
    11101 acatggtagg ttaatacctt gttagttatt gctagtcact gactagtcaa taactagtct
    11161 ctgaacacct tgcaatcact ccgtttgcgc agctgaacag tggtgaggat aatagtagta
    11221 agttattcta gcttcagagt tataggagac tagatactaa ctagtattag ttgcaactaa
    11281 cctggatctc agagactttg gcctcaatct agaatctatc tagttgtcaa ctagactgtg
    11341 gtatcattgt cttttatttt cctagtcctg gaactagctt ctaactagtc tccctaatat
    11401 gtggctgtct tgtttttttt ttttgtttcc ctacccggat atctagtccc cttctaggtt
    11461 ctgttaacct ctcgggctct gatttagttt aacgcaaacc tgagattagt ttctaactag
    11521 tctctaggtt ttctatccac ctttaattgt aataataaat acaagcaacg tttatacgtc
    11581 aaaagcattt ataaactttt accctaaagt agcttgcttg tgtgtttagt ttataattag
    11641 tctcttatta atttgatgta ggtaagcccg ccacaaatat atatttttaa caagataccg
    11701 tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa taagctggct ttccccgtca
    11761 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    11821 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    11881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    11941 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
    12001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatggg
    12061 gtgacgatga gccgctcttg catctttgtt tgtattatac tgtctttctt gttacacata
    12121 attattctag aatgccccac cgttacatac gggacacagc catttacata tgcatgtgga
    12181 ttacgagcta acgagttcat tcaaatctca gaactatcac ataatcatca ttcccctatc
    12241 gtcaaagacc gtaagacaaa tccggttcat gcactgaacc cattcgggta gtgagtcatt
    12301 tactcagcac actcgcgctg acgctcgtcg aacaccttca atgcctcctc ggcagccttg
    12361 acaccactga gaaccatggc accgaaggta gggcccatgc ggttaaagcc atcaatttca
    12421 gacagctcca taccgccgat tatcaagccc ttagtaacct cgcgggtgtt cttgacgatg
    12481 gcatcctgcg ctgattgtga tagttcccac ttgtccgtcc gcatcggcat ccgcagctcg
    12541 ggatagttcc gacctaggat tggatgcatg cggaaccgca cgagggcggg gcggaaattg
    12601 acacaccact cctctccacg caccgttcaa gaggtacgcg tatagagccg tatagagcag
    12661 agacggagca ctttctggta ctgtccgcac gggatgtccg cacggagagc cacaaacgag
    12721 cggggccccg tacgtgctct cctaccccag gatcgcatcc ccgcatagct gaacatctat
    12781 ataaagaccc ccaaggttct cagtctcacc aacatcatca accaacaatc aacagttctc
    12841 tactcagtta attagaactc ttccaatcct atcacctcgc cttaattaag cggaacaaat
    12901 tgaagcgagt ccccgtggag cgcgagcccg cgagagagtc gtgcgggatg atgccatgaa
    12961 gtatcaccta cattgtatgc tctccagatc gaagaggaat actctgaata agtggattta
    13021 ggcggtttct ttggtcctac tatatgcaca ctcagattct gtcaattgaa aagtcagccc
    13081 gctgtctatc atgacttcta tctccattaa ttgtcatcca gccgtgcctc tgtatggtgc
    13141 gtataccctc gactcacacc tgtgaagtct ctgccactta gcgcagccaa agaagattta
    13201 gtctaaaggt ctacaatcaa ttcaggccgt attcagggcg gccgcttctg ctgtttctgg
    13261 gctcaatgct ttggtatctc aaaggagagg agaagcgtcc gtggaaggca ggaagaccta
    13321 caagacctgg cactcagaaa gagggcggtt gctcttaagt gtagggctgc gcaggggagg
    13381 aactctgggg ttgtcaaatg cggcagacgg aaatctacat tagcagcgtc aatccacccc
    13441 cttttggtga accataaagg ggctagtcta ccccagcttc gaatcagaat aatataaatg
    13501 cggggaaagc tttaggtatg cggacagatc catactgggt cctccaagag atcggcccgc
    13561 acatctgacg tggtttcaag gttcgaccca tgcggagccg cattggagga tctatccctg
    13621 taggtacctt agctaaagat ctacgccgta tggccaagcg gaagatatag gaacgatcgg
    13681 ccatgaagtc gtgtcttcta caaactctcg tatattcact aggttcgacg tctgctaaac
    13741 taacctgacc taacctaacc ttaactccct gccagttcag cccatgcgga cccggcagtc
    13801 atgtctttcg cttcagattg aacgcggctg cctcgtctcg atcaggattt agcagaagac
    13861 agtacacact gaaaaagtac cacaataaag aagctatatt gcaggccaaa tagccgtctg
    13921 ctcggtctgt ttattcaacc tggcctcttt catcgttttt cccttttcct tattaaacca
    13981 tatatctcca gcagagcgat cgcgggagga gataatttag ttgagatcga agttggcttt
    14041 atagttgtaa cagtgaaatc agggcacgaa tctttagtac ttcgttgatc gccactccac
    14101 caagggtttg ccattaagca taactggcaa tatttttgtc aatttgcctt ttcgaccgtg
    14161 taaatgtccc tatccatact ctccgcagtc aatatccgga ggtcaagtgt gaagactgtt
    14221 taccccagca agatacttgg acaagcctgg tttgcaatcc atagtttcag caggcttcct
    14281 aggtggcctt aagcctggga tactggctgg ctgctagcaa ttgccatgac tatgttggag
    14341 gaaatgttcg agaggaatct tgggtccatg aacagagtag tcccacttca aatgttgcgt
    14401 atgcctgata ttacggacca atagaggaac aacctggcag cagctaactt gtatatctct
    14461 aatcgcgttg acgaccgttc tgtgtcagtg caactgccac gcaaagactt cagaataata
    14521 aactttgcca gagaaggcag ttgtattatg ttaatatcgc aacattgtga aaattaaatg
    14581 aactaacaat caaaaagaaa gaaaatccga actttccctc cgtattatct aacccgtgtg
    14641 cttttgcaac gcaatccaac tattccagtt gacgacatta atccaagcca gcataggaag
    14701 aataccaaaa accttcagac agccgtaccc gcaagccttg ttaccaacca gctatagcaa
    14761 tcttacccgt tcaatttctt aatccccatc tagacgcttc cagcactctg actgtcggga
    14821 gacgggttcc tttgccagcc atgagcattc gcctagttag ggagcggcgg atgcctactt
    14881 acttctggga acgaggcgcg ccgtgagcgg gaaatatggg tatcttggga gaggagagtt
    14941 ggagtaggga gaaatgctgg ctcggtggga ggacgaggga gctttaataa taccgctctg
    15001 gctttggtag cactgagcca gtggtcgcag caaaagaggg gaaaggaggg gaacaggtca
    15061 agcggagccg ctcttgttga ggatagctgt tacccatgga aattacatgt agcaagctag
    15121 caacccgccg aagtgcccca ctggtcttca agagccagtc caggtgaact gcattccgtc
    15181 cccttcgccg cggtgcccac tgcctccaca gcctagatga ttctgaaaac acgttgaaga
    15241 gctcgcgata ataaccgagg cgggtaatgc aggatgaaac ttacgatcta caacctacag
    15301 caccctggtt ctgaaacaaa gttgaggatc cccgcgatcg ataacccaga ccgtaatgcg
    15361 aattgcaatt tacgatttac cccccgtaga gcggactgat tctggaccta tcttgagact
    15421 atgtggtgga gcctgcgatg ataaccgagg ccgtaatgca aaaatgaaaa ttacgaacca
    15481 agaggcgtga tgagaagatc a
//
