LOCUS       sequence-353768-       10669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0069
FEATURES             Location/Qualifiers
     source          1..10669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3607..4195
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3660..3680)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4078..4089)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4267..4500
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4578..4699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4615..4634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4669..4688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4739..4874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4894..4913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4895..4913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4923..4940)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4923..4939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4932..4954)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5081..5536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5168..5187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5378..5399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5562..5666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5667..5735,5736..6527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5885..5904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6698..7286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7187..7206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7440..7457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7574..7595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7610..7615,7616..7633,7634..7640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7648..7664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7653..7675
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7672..7688
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7672..7688
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7707..7727
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7709..7727
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7755..7981
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7982..8162
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8209..8334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8827..9060
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9245..9289
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9520..9730
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9542..9738
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9589..9724
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9770..10372
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9770..9789)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10266..10286
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10415..10532
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgag
     1201 ctttgatcca aaccttctcc acaacaatgg acataatggg taccctaatg gtacttcagc
     1261 agcactgcgt gaaactgggg ttattgaaaa actgttaacc tcttacggat ttattcagtg
     1321 ttcagaacgt caagctagac ttttcttcca ctgttcacag tataatggca acctgcaaga
     1381 cttaaaagta ggagatgatg ttgaatttga agtatcatcg gaccgacgga ctgggaaacc
     1441 cattgctgtt aaactggtga agataaaaca agaaatcctc cctgaagaac gaatgaatgg
     1501 acaagaagtg ttttatctga cttacacccc tgaagatgtc gaagggaacg ttcagctgga
     1561 aactggagat aaaataaact ttgtaattga taacaataaa catactggtg ctgtaagtgc
     1621 tcgcaacatt atgctgttga aaaagaaaca agcccgctgt cagggagtag tttgtgccat
     1681 gaaggaggca tttggcttta ttgaaagagg tgatgttgta aaagagatat tctttcacta
     1741 tagtgaattt aagggtgact tagaaacctt acagcctggc gatgatgtgg aattcacaat
     1801 caaggacaga aatggtaaag aagttgcaac agatgtcaga ctattgcctc aaggaacagt
     1861 catttttgaa gatatcagca ttgaacattt tgaaggaact gtaaccaaag ttatcccaaa
     1921 agtacccagt aaaaaccaga atgacccatt gccaggacgc atcaaagttg actttgtgat
     1981 ccctaaagaa cttccctttg gagacaaaga tacgaaatcc aaggtgaccc tgctggaagg
     2041 tgaccatgtt aggtttaata tttcaacaga ccgacgtgac aaattagagc gagcaaccaa
     2101 tatagaagtt ctgtcaaata catttcagtt cactaatgaa gcccgagaaa tgggtgtgat
     2161 tgctgccatg agagatggtt ttggtttcat caagtgtgtg gatcgtgatg ttcgtatgtt
     2221 cttccacttc agtgaaattc tggatgggaa ccagctccat attgcagatg aagtagagtt
     2281 tactgtggtt cctgatatgc tctctgctca aagaaatcat gctattagga ttaaaaaact
     2341 tcccaagggc acggtttcat ttcattccca ttcagatcac cgttttctgg gcacggtaga
     2401 aaaagaagcc actttttcca atcctaaaac cactagccca aataaaggca aagagaagga
     2461 ggctgaggat ggcattattg cttatgatga ctgtggggtg aaactgacta ttgcttttca
     2521 agccaaggat gtggaaggat ctacttctcc tcaaatagga gataaggttg aatttagtat
     2581 tagtgacaaa cagaggcctg gacagcaggt tgcaacttgt gtgcgacttt taggtcgtaa
     2641 ttctaactcc aagaggctct tgggttatgt ggcaactctg aaggataatt ttggatttat
     2701 tgaaacagcc aatcatgata aggaaatctt tttccattac agtgagttct ctggtgatgt
     2761 tgatagcctg gaactggggg acatggtcga gtatagcttg tccaaaggca aaggcaacaa
     2821 agtcagtgca gaaaaagtga acaaaacaca ctcagtgaat ggcattactg aggaagctga
     2881 tcccaccatt tactctggca aagtaattcg ccccctgagg agtgttgatc caacacagac
     2941 tgagtaccaa ggaatgattg agattgtgga ggagggcgat atgaaaggtg aggtctatcc
     3001 atttggcatc gttgggatgg ccaacaaagg ggattgcctg cagaaagggg agagcgtcaa
     3061 gttccaattg tgtgtcctgg gccaaaatgc acaaactatg gcttacaaca tcacacccct
     3121 gcgcagggcc acagtggaat gtgtgaaaga tcagtttggc ttcattaact atgaagtagg
     3181 agatagcaag aagctctttt tccatgtgaa agaagttcag gatggcattg agctacaggc
     3241 aggagatgag gtggagttct cagtgattct taatcagcgc actggcaagt gcagcgcctg
     3301 taatgtttgg cgagtctgtg agggccccaa ggctgttgca gctcctcgac ctgatcggtt
     3361 ggtcaatcgc ttgaagaata tcactctgga tgatgccagt gctcctcgcc taatggttct
     3421 tcgtcagcca aggggaccag ataactcaat ggggtttggt gcagaaagaa agatccgtca
     3481 agctggtgtc attgactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     3541 gctttatata tcttgtggaa aggacgagcc tgctgtgaaa ggaaatggcg cacgcgttaa
     3601 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3661 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3721 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3781 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3841 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3901 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3961 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4021 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4081 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4141 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     4201 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4261 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4321 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4381 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4441 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4501 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4561 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4621 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4681 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4741 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4801 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4861 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4921 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4981 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5041 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5101 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5161 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5221 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5281 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5341 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5401 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5461 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5521 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7501 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7561 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7621 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7681 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7741 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7801 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7861 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7921 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7981 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8041 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8101 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8161 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8221 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8281 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8341 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8401 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8461 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8521 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8581 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8641 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8701 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8761 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8821 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8881 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8941 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9001 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9061 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9121 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9181 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9241 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9301 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9361 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9421 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9481 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9541 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9601 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9661 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9721 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     9781 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9841 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9901 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9961 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
    10021 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    10081 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    10141 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    10201 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    10261 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    10321 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10381 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10441 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10501 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10561 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    10621 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
