LOCUS       sequence-353771-       10081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0147
FEATURES             Location/Qualifiers
     source          1..10081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3019..3607
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3072..3092)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3490..3501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3679..3912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3990..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4027..4046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4081..4100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4151..4286
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4306..4325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4307..4325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4335..4352)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4335..4351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4344..4366)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4493..4948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4580..4599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4790..4811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4974..5078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5079..5147,5148..5939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5297..5316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6110..6698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6599..6618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6852..6869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6986..7007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7022..7027,7028..7045,7046..7052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7060..7076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7065..7087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7084..7100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7084..7100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7119..7139
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7121..7139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7167..7393
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7394..7574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7621..7746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8239..8472
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8657..8701
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8932..9142
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8954..9150
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9001..9136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9182..9784
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9182..9201)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9678..9698
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9827..9944
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 gctcaccagc tttttacctg cacctactca gctatctcag gaccagcttg aggctgaaga
     1261 aaaggcaaga tcccagagat cacggcagac ctcactggtc tcctcccgaa gagaacctcc
     1321 cccgtacgga taccggaaag gctggatacc tcggttatta gaggattttg gagatggagg
     1381 tgcttttcca gagatccatg tggcccagta tccactggat atgggacgaa agaaaaaaat
     1441 gtcgaatgcg ctggccattc aggtggattc tgaaggaaaa attaaatatg atgcaattgc
     1501 tcgacaagga cagtcaaaag acaaggtcat ttatagcaaa tacactgacc tggttccaaa
     1561 ggaggttatg aatgcagatg atccagacct gcaaaggccc gatgaagaag ctattaaaga
     1621 gataacagaa aagacaagag tagccttaga aaaatctgta tcacagaagg tcgccgcagc
     1681 catgccagtt cgagcagctg acaaattggc tcctgctcag tatatccgat acacaccatc
     1741 tcagcaagga gtggcattca actctggagc taaacagagg gttattcgga tggtagaaat
     1801 gcagaaagat ccaatggagc ctccaaggtt caagattaat aagaaaattc cccggggacc
     1861 accttctcct cctgcgcctg tcatgcattc tcctagccga aagatgactg taaaggaaca
     1921 acaagagtgg aagattcctc cttgtatttc taactggaaa aatgcaaagg gttatacaat
     1981 tccattagac aaacgtctgg ctgctgatgg aagaggacta cagacagtac acataaatga
     2041 aaatttcgcc aaattggcag aagccctcta cattgctgat cggaaggctc gtgaagctgt
     2101 ggaaatgcgt gcccaagtag agagaaaaat ggctcagaaa gaaaaggaaa aacatgaaga
     2161 gaaacttaga gaaatggccc agaaagccag ggagagaaga gctgggatca aaactcatgt
     2221 ggaaaaagag gatggggagg cacgtgagag ggatgaaatc cggcatgaca ggcgaaaaga
     2281 gagacagcat gaccggaatc tttccagggc agctcctgat aagaggtcga aacttcagag
     2341 aaatgaaaat cgggatatca gtgaagttat tgctctcggt gttcctaatc ctcggacttc
     2401 caatgaagtt cagtatgacc aaaggctctt caaccaatcc aagggtatgg acagtggatt
     2461 tgcaggtgga gaagatgaaa tttataatgt ttatgatcaa gcctggagag gtggtaaaga
     2521 tatggcccag agtatttata ggcccagtaa aaatctggac aaggacatgt atggtgatga
     2581 cctagaagcc agaataaaga ccaacaggtg ccaagccata caactcaatt tcagtgttta
     2641 cactggtgaa agcaaagtag ttcatagttt tttctccttt tccttagatt tgttcccgac
     2701 aaggagtttt ctggttcaga ccgtagacag agaggccgag aaggaccagt gcagtttgag
     2761 gaagatcctt ttggtttgga caagtttttg gaagaagcca aacagcatgg tggctctaaa
     2821 agaccctcag atagcagccg ccccaaggaa cacgagcatg aaggcaagaa gaggaggaag
     2881 gaataggcac aggtctctcc aaagtgaata gtaatgaact agtccgtaac ttgaaagtat
     2941 ttcgatttct tggctttata tatcttgtgg aaaggacgac gtccgtcgtt tcacctgtac
     3001 tacacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3061 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3121 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3181 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3241 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3301 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3361 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3421 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3481 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3541 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3601 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3661 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3721 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3781 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3841 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3901 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3961 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4021 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4081 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4141 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4201 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4261 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4321 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4381 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4441 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4501 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4561 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4621 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4681 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4741 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4801 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4861 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4921 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4981 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5041 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5101 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5161 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5221 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5281 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5341 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5401 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5461 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5521 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5581 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5641 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5701 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5761 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5821 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5881 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5941 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6001 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6061 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6121 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6181 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6241 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6301 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6361 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6421 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6481 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6541 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6601 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6661 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6721 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6781 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6841 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6901 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6961 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7021 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7081 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7141 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7201 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7261 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7321 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7381 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7441 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7501 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7561 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7621 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7681 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7741 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7801 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7861 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7921 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7981 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8041 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8101 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8161 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8221 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8281 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8341 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8401 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8461 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8521 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8581 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8641 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8701 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8761 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8821 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     8881 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8941 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9001 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9061 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9121 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9181 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9241 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9301 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9361 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     9421 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9481 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9541 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9601 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9661 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9721 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     9781 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9841 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9901 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9961 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    10021 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    10081 t
//
