LOCUS       sequence-353776-       10861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2195
FEATURES             Location/Qualifiers
     source          1..10861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3799..4387
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3852..3872)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4270..4281)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4459..4692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4770..4891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4807..4826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4861..4880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4931..5066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5086..5105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5087..5105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5115..5132)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5115..5131)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5124..5146)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5273..5728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5360..5379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5570..5591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5754..5858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5859..5927,5928..6719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6077..6096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6890..7478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7379..7398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7632..7649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7766..7787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7802..7807,7808..7825,7826..7832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7840..7856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7845..7867
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7864..7880
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7864..7880
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7899..7919
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7901..7919
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7947..8173
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8174..8354
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8401..8526
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9019..9252
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9437..9481
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9712..9922
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9734..9930
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9781..9916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9962..10564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9962..9981)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10458..10478
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10607..10724
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 cggcgcgctg gcaggtctgg ccgcgggctt gcaggtcccg cgggtcgcgc ccagcccaga
     1261 ctcggactcg gacacagact cggaggaccc gagtctccgg cgcagcgcgg gcggcttgct
     1321 ccgctcgcag gtcatccaca gcggtcactt catggtgtcg tcgccgcaca gcgactcgct
     1381 gccccggcgg cgcgaccagg aggggtccgt ggggccctcc gacttcgggc cgcgcagtat
     1441 cgaccccaca ctcacacgcc tcttcgagtg cttgagcctg gcctacagtg gcaagctggt
     1501 gtctcccaag tggaagaatt tcaaaggcct caagctgctc tgcagagaca agatccgcct
     1561 gaacaacgcc atctggaggg cctggtatat ccagtatgtg aagcggagga agagccccgt
     1621 gtgtggcttc gtgacccccc tgcaggggcc tgaggctgat gcgcaccgga agccggaggc
     1681 cgtggtcctg gaggggaact actggaagcg gcgcatcgag gtggtgatgc gggaatacca
     1741 caagtggcgc atctactaca agaagcggct ccgtaagccc agcagggaag atgacctcct
     1801 ggcccctaag caggcggaag gcaggtggcc gccgccggag caatggtgca aacagctctt
     1861 ctccagtgtg gtccccgtgc tgctggggga cccagaggag gagccgggtg ggcggcagct
     1921 cctggacctc aattgctttt tgtccgacat ctcagacact ctcttcacca tgactcagtc
     1981 cggcccttcg cccctgcagc tgccgcctga ggatgcctac gtcggcaatg ctgacatgat
     2041 ccagccggac ctgacgccac tgcagccaag cctggatgac ttcatggaca tctcagattt
     2101 ctttaccaac tcccgcctcc cacagccgcc catgccttca aacttcccag agccccccag
     2161 cttcagcccc gtggttgact ccctcttcag cagtgggacc ctgggcccag aggtgccccc
     2221 ggcttcctcg gccatgaccc acctctctgg acacagccgt ctgcaggctc ggaacagctg
     2281 ccctggcccc ttggactcca gcgccttcct gagttctgat ttcctccttc ctgaagaccc
     2341 caagccccgg ctcccacccc ctcctgtacc cccacctctg ctgcattacc ctccccctgc
     2401 caaggtgcca ggcctggagc cctgcccccc acctcccttc cctcccatgg caccacccac
     2461 tgctttgctg caggaagagc ctctcttctc tcccaggttt cccttcccca ccgtccctcc
     2521 tgccccagga gtgtctccgc tgcctgctcc tgcagccttc ccacccaccc cacagtctgt
     2581 ccccagccca gcccccaccc ccttccccat agagcttcta cccttggggt attcggagcc
     2641 tgcctttggg ccttgcttct ccatgcccag aggcaagccc cccgccccat cccctagggg
     2701 acagaaagcc agccccccta ccttagcccc tgccactgcc agtcccccca ccactgcggg
     2761 gagcaacaac ccctgcctca cacagctgct cacagcagct aagccggagc aagccctgga
     2821 gccaccactt gtatccagca ccctcctccg gtccccaggg tccccgcagg agacagtccc
     2881 tgaattcccc tgcacattcc ttcccccgac cccggcccct acaccgcccc ggccacctcc
     2941 aggcccggcc acattggccc cttccaggcc cctgcttgtc cccaaagcgg agcggctctc
     3001 acccccagcg cccagcggca gtgaacggcg gctgtcaggg gacctcagct ccatgccagg
     3061 ccctgggact ctgagcgtcc gtgtctctcc cccgcaaccc atcctcagcc ggggccgtcc
     3121 agacagcaac aagaaccggc gtatcacaca catctccgcg gagcagaagc ggcgcttcaa
     3181 catcaagctg gggtttgaca cccttcatgg gctcgtgagc acactcagtg cccagcccag
     3241 cctcaaggag cgtgcgggct tgcaggagga ggcccagcag ctgcgggatg agattgagga
     3301 gctcaatgcc gccattaacc tgtgccagca gcagctgccc gccacagggg tacccatcac
     3361 acaccagcgt tttgaccaga tgcgagacat gtttgatgac tacgtccgaa cccgtacgct
     3421 gcacaactgg aagttctggg tgttcagcat cctcatccgg cctctgtttg agtccttcaa
     3481 cgggatggtg tccacggcaa gtgtgcacac cctccgccag acctcactgg cctggctgga
     3541 ccagtactgc tctctgcccg ctctccggcc aactgtcctg aactccctac gccagctggg
     3601 cacatctacc agtatcctga ccgacccggg ccgcatccct gagcaagcca cacgggcagt
     3661 cacagagggc acccttggca aacctttata gtaatgaact agtccgtaac ttgaaagtat
     3721 ttcgatttct tggctttata tatcttgtgg aaaggacgat gccgtgagaa tgacatacca
     3781 agcacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4201 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4381 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4441 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     4501 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     4561 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4621 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4681 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4741 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4801 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4861 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4921 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4981 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5041 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5101 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5161 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5221 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5341 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5461 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5581 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5641 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5701 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5761 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5821 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5881 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5941 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6001 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6061 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6121 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6181 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6241 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6301 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6361 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6421 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6481 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6541 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6601 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6661 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6721 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7501 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7561 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7621 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7681 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7741 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7801 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7861 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7921 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7981 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     8041 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     8101 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     8161 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8221 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8281 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8341 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8401 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8461 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8521 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8581 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8641 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8701 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8761 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8821 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8881 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8941 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9001 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9061 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9121 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9181 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9241 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9301 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9361 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9421 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9481 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9541 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9601 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     9661 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9721 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9781 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9841 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9901 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9961 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
    10021 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
    10081 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    10141 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
    10201 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    10261 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    10321 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    10381 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    10441 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    10501 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
    10561 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10621 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10681 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10741 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    10801 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    10861 t
//
