LOCUS       sequence-353779-       11965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2805
FEATURES             Location/Qualifiers
     source          1..11965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4631..4651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    4903..5491
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4956..4976)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5374..5385)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5563..5796
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5874..5995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5911..5930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5965..5984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6035..6170
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6190..6209)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6191..6209)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6219..6236)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6219..6235)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6228..6250)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6377..6832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6464..6483)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6674..6695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6858..6962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6963..7031,7032..7823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7181..7200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7994..8582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8483..8502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8736..8753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8870..8891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8906..8911,8912..8929,8930..8936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8944..8960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8949..8971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8968..8984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8968..8984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9003..9023
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9005..9023
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9051..9277
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9278..9458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9505..9630
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10123..10356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10541..10585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10816..11026
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10838..11034
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10885..11020
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11066..11668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11066..11085)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11562..11582
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11711..11828
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 gctgactggt gtagagcccg ccagaatgaa caggaagaaa ggagacaagg gctttgaaag
     1261 cccaaggcca tataaattaa cccatcaggt cgtctgcatc aacaacataa atttccagag
     1321 aaaatctgtt gtgggatttg tggaactgac tatatttccc acagttgcaa acttgaatag
     1381 aatcaagttg aacagcaaac agtgtagaat ataccgagta aggatcaatg atttagaggc
     1441 tgcttttatt tataatgacc caaccttgga agtttgtcac agtgaatcaa aacagagaaa
     1501 cctcaattat ttttccaatg cttatgcagc tgcagttagt gctgtggacc ctgatgcagg
     1561 aaatggagaa ctttgcatta aggttccatc agagctatgg aaacacgttg atgagttaaa
     1621 ggtcctgaag atacacatca atttttcttt ggatcagccc aaaggaggtc ttcattttgt
     1681 ggtacccagt gtagagggaa gtatggcaga gagaggtgct catgttttct cttgtgggta
     1741 tcaaaattct acaagatttt ggttcccttg tgttgattca tactctgaat tgtgtacatg
     1801 gaaattagaa tttacagtag atgctgcaat ggttgctgtt tctaatggcg atttggtgga
     1861 gacagtgtat actcatgata tgaggaagaa aactttccat tatatgctta ccattcctac
     1921 agcagcgtca aatatctcct tggccattgg accatttgaa atactggtag atccatacat
     1981 gcatgaggtt actcattttt gtttgcccca acttcttcca ttgctgaaac ataccacatc
     2041 ataccttcat gaagtctttg aattttatga agaaattctt acatgtcgtt acccatactc
     2101 ctgttttaag actgtcttca ttgatgaggc ttatgttgaa gtggctgctt atgcttccat
     2161 gagcattttt agcacaaatc ttttacacag tgccatgatt atagatgaga cacctttgac
     2221 tagaaggtgt ttagcccaat ccttggccca gcagtttttt ggttgtttca tatctagaat
     2281 gtcttggtct gatgaatggg tgctgaaggg aatttcaggc tatatctatg gactttggat
     2341 gaaaaaaact tttggtgtta atgagtaccg ccattggatt aaagaggagc tagacaaaat
     2401 agtggcatat gaactaaaaa ctggtggggt tttactacat cccatatttg gtggaggaaa
     2461 agagaaggat aatccggctt cccatctaca cttttcaata aagcatccac atacactgtc
     2521 ctgggaatac tacagtatgt ttcagtgtaa agcccacctt gtgatgagat tgattgaaaa
     2581 taggatcagt atggaattta tgctacaagt tttcaataaa ctgctaagtc tggctagtac
     2641 tgcttcatct cagaagttcc agtcacatat gtggagtcag atgttggttt ccacatctgg
     2701 gtttttgaaa tccatttcaa atgtctctgg caaagatatt cagccgttaa taaagcagtg
     2761 ggtagatcag agtggagtgg taaaatttta tggaagtttt gcatttaata gaaaacgaaa
     2821 tgtcttggaa ctggaaataa aacaggacta tacatctcct ggaactcaga aatacgtggg
     2881 accacttaaa gtgacagtgc aggagttaga tggatccttc aatcatacac tgcaaattga
     2941 agaaaacagc cttaaacatg atataccctg ccattccaaa agtagaagga ataaaaagaa
     3001 aaaaatccca ctgatgaatg gagaagaagt tgatatggat ctttctgcaa tggatgctga
     3061 ttcccctttg ctgtggataa ggatagaccc agatatgtca gtattgagga aggtagaatt
     3121 tgagcaagct gattttatgt ggcagtatca gctccgctat gagagagatg ttgttgcaca
     3181 gcaggaatcc attttggctt tggaaaaatt ccctactcca gcatctcggc ttgcactcac
     3241 tgatatatta gaacaagagc agtgtttcta cagagtaaga atgtcagctt gcttctgtct
     3301 tgcaaagatt gcaaattcaa tggtgagcac atggacagga ccaccagcca tgaagtcact
     3361 cttcactagg atgttttgtt gtaaaagttg tccaaacatt gtgaaaacaa acaactttat
     3421 gagctttcaa agctattttc tacagaagac tatgccagtt gcaatggctt tattaagaga
     3481 tgttcataat ctttgtccta aagaagtctt aacatttatt ttagacttaa tcaagtacaa
     3541 tgacaacagg aaaaataagt tttcagataa ctattatcgt gcagaaatga ttgatgccct
     3601 ggccaactct gttacacctg cagtcagtgt gaataatgaa gttagaactt tggataactt
     3661 aaatcctgat gtgcgactca ttcttgaaga aatcaccaga tttttgaata tggaaaaact
     3721 tcttccgagt tacaggcata ccatcactgt cagttgtttg agagccatac gggtacttca
     3781 gaagaacgga catgtgccaa gtgatccagc tctttttaaa tcttatgctg aatatggcca
     3841 ctttgtggac attaggatag cagctttgga agcagttgtt gattatacta aagtggacag
     3901 aagttatgaa gaactgcaat ggctacttaa tatgattcag aatgaccctg taccctatgt
     3961 aaggcataag attctcaaca tgttgactaa gaacccacca tttactaaga acatggagtc
     4021 tcccttatgc aatgaagccc tggtagatca actttggaaa cttatgaatt ctggtacttc
     4081 acatgactgg aggttacggt gtggtgctgt ggacttgtac ttcacacttt ttggcctcag
     4141 tagaccttcc tgtttaccct tgccagagct tgggttggtt cttaatctaa aggagaaaaa
     4201 agctgtcttg aatcctacca taattccaga gtcagtagca ggcaaccaag aagctgcaaa
     4261 taatccaagc agtcacccac agctagttgg atttcagaac cctttttcca gttctcaaga
     4321 tgaggaggag attgatatgg atactgttca tgatagccag gccttcattt cccatcattt
     4381 aaacatgctt gaaaggccgt caactccagg gctctcgaaa tatcggccag ctagctcccg
     4441 atctgcttta ataccccagc actcagcagg ctgtgacagc acacccacca caaaacccca
     4501 gtggagtttg gaacttgcac ggaagggaac aggtaaagaa caagcacctt tggagatgag
     4561 tatgcatcca gcggcaagcg ctccactctc agtctttact aaggaatcta cagcctccaa
     4621 acacagtgac caccatcacc accatcacca tgagcacaag aaaaagaaga agaagcataa
     4681 acataagcac aaacacaagc ataagcatga cagtaaagaa aaggacaagg agcctttcac
     4741 tttctccagc cctgccagtg gcaggtctat tcgttctcct tccctttcag actagtaatg
     4801 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4861 cgatattgga gtcagtgagc cattcacacg cgttaagtcg acaatcaacc tctggattac
     4921 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4981 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5041 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5101 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5161 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5221 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5281 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5341 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5401 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5461 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     5521 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5581 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     5821 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6001 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6061 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6121 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6181 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6361 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6421 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6481 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6541 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6601 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6661 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6721 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6781 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9001 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     9061 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     9121 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     9181 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     9241 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     9301 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9361 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9421 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9481 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9541 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9601 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9661 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9721 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9781 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9841 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9901 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9961 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
    10021 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
    10081 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
    10141 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
    10201 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
    10261 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
    10321 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
    10381 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    10441 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    10501 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    10561 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    10621 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    10681 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10741 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
    10801 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
    10861 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10921 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10981 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
    11041 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
    11101 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    11161 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
    11221 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    11281 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    11341 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    11401 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    11461 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    11521 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    11581 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    11641 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    11701 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11761 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11821 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    11881 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    11941 ctaggtcttg aaaggagtgg gaatt
//
