LOCUS       sequence-353783-       12188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for recombinase mediated integration in filamentous 
            fungi. Is a shuttle vector for optional in vivo cloning in yeast, 
            and contains four inducible promoters or sites for whole BGC 
            cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Roux I, Chooi YH
  TITLE     Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene 
            Clusters for Heterologous Expression in Aspergillus nidulans.
  JOURNAL   ACS Synth Biol. 2022 Feb 16. doi: 10.1021/acssynbio.1c00458.
  PUBMED    35168324
REFERENCE   2  (bases 1 to 12188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Feb 16. doi: 10.1021/acssynbio.1c00458."
COMMENT     Sequence Label: pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66
FEATURES             Location/Qualifiers
     source          1..12188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    139..172
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(952..968)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(953..969)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             998..1708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKGGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1143..1161)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1411..1430
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(7527..7595,7596..8387)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7745..7764)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8558..9146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9047..9066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9300..9317
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9434..9455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9470..9475,9476..9493,9494..9500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9508..9524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9513..9535
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9532..9548
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9532..9548
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      9565..10907
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10528..10575)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10948..10966
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11004..11026)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11126..11145
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11169..11384
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11385..12188
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa
       61 ttatatcagt tattacccta tgcggtgtga aataccgcac agatgcgtaa ggagagtcga
      121 ctctagagga tccccgggat aacttcgtat aatgtatgct atacgaagtt atggattttc
      181 ttaacttctt cggcgacagc atcaccgact tcgcgatcgc atgcgggaga cggacggacg
      241 cagagagaag ggctgagtaa taagcgccac tgcgccagac agctctggcg gctctgaggt
      301 gcagtggatg attattaatc cgggaccggc cgcccctccg ccccgaagtg gaaaggctgg
      361 tgtgcccctc gttgaccaag aatctattgc atcatcggag aatatggagc ttcatcgaat
      421 caccggcagt aagcgaagga gaatgtgaag ccaggggtgt atagccgtcg gcgaaatagc
      481 atgccattaa cctaggtaca gaagtccaat tgcttccgat ctggtaaaag attcacgaga
      541 tagtaccttc tccgaagtag gtagagcgag tacccggcgc gtaagctccc taattggccc
      601 atccggcatc tgtagggcgt ccaaatatcg tgcctctcct gctttgcccg gtgtatgaaa
      661 ccggaaaggc cgctcaggag ctggccagcg gcgcagaccg ggaacacaag ctggcagtcg
      721 acccatccgg tgctctgcac tcgacctgct gaggtccctc agtccctggt aggcagcttt
      781 gccccgtctg tccgcccggt gtgtcggcgg ggttgacaag gtcgttgcgt cagtccaaga
      841 tttgttgcca tattttcctg ctctccccac cagctgctct tttcttttct ctttcttttc
      901 ccatcttcag tatattcatc ttcccatcca agaaccttta atcaagctta tcgataccgt
      961 cgacctcgac tctagaggat cactcgtcgt gtcgcccatg gtgagcaagg gcgaggagga
     1021 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1081 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1141 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1201 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1261 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1321 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1381 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1441 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1501 gaggctgaag ctgaagggcg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1561 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1621 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1681 cggcggcatg gacgagctgt acaagtaatg ggcgacgacg acgttcgagg gggggcccgg
     1741 taccgccccg tccggtcctg cccgtcaccg agatccactt aacgttactg aaatcatcaa
     1801 acagcttgac gaatctggat ataagatcgt tggtgtcgat gtcagctccg gagttgagac
     1861 aaatggtgtt caggatctcg ataagatacg ttcatttgtc caagcagcaa agagtgcctt
     1921 ctagtgattt aatagctcca tgtcaacaag aataaaacgc gtttcgggtt tacctcttcc
     1981 agatacagct catctgcaat gttaagaatt cgctgattgt gatagttccc acttgtccgt
     2041 ccgcatcggc atccgcagct cgggatagtt ccgacctagg attggatgca tgcggaaccg
     2101 cacgagggcg gggcggaaat tgacacacca ctcctctcca cgcaccgttc aagaggtacg
     2161 cgtatagagc cgtatagagc agagacggag cactttctgg tactgtccgc acgggatgtc
     2221 cgcacggaga gccacaaacg agcggggccc cgtacgtgct ctcctacccc aggatcgcat
     2281 ccccgcatag ctgaacatct atataaagac ccccaaggtt ctcagtctca ccaacatcat
     2341 caaccaacaa tcaacagttc tctactcagt taattagaac tcttccaatc ctatcacctc
     2401 gccttaatta agcggaacaa attgaagcga gtccccgtgg agcgcgagcc cgcgagagag
     2461 tcgtgcggga tgatgccatg aagtatcacc tacattgtat gctctccaga tcgaagagga
     2521 atactctgaa taagtggatt taggcggttt ctttggtcct actatatgca cactcagatt
     2581 ctgtcaattg aaaagtcagc ccgctgtcta tcatgacttc tatctccatt aattgtcatc
     2641 cagccgtgcc tctgtatggt gcgtataccc tcgactcaca cctgtgaagt ctctgccact
     2701 tagcgcagcc aaagaagatt tagtctaaag gtctacaatc aattcaggcc gtattcaggg
     2761 cggccgcttc tgctgtttct gggctcaatg ctttggtatc tcaaaggaga ggagaagcgt
     2821 ccgtggaagg caggaagacc tacaagacct ggcactcaga aagagggcgg ttgctcttaa
     2881 gtgtagggct gcgcagggga ggaactctgg ggttgtcaaa tgcggcagac ggaaatctac
     2941 attagcagcg tcaatccacc cccttttggt gaaccataaa ggggctagtc taccccagct
     3001 tcgaatcaga ataatataaa tgcggggaaa gctttaggta tgcggacaga tccatactgg
     3061 gtcctccaag agatcggccc gcacatctga cgtggtttca aggttcgacc catgcggagc
     3121 cgcattggag gatctatccc tgtaggtacc ttagctaaag atctacgccg tatggccaag
     3181 cggaagatat aggaacgatc ggccatgaag tcgtgtcttc tacaaactct cgtatattca
     3241 ctaggttcga cgtctgctaa actaacctga cctaacctaa ccttaactcc ctgccagttc
     3301 agcccatgcg gacccggcag tcatgtcttt cgcttcagat tgaacgcggc tgcctcgtct
     3361 cgatcaggat ttagcagaag acagtacaca ctgaaaaagt accacaataa agaagctata
     3421 ttgcaggcca aatagccgtc tgctcggtct gtttattcaa cctggcctct ttcatcgttt
     3481 ttcccttttc cttattaaac catatatctc cagcagagcg atcgcgggag gagataattt
     3541 agttgagatc gaagttggct ttatagttgt aacagtgaaa tcagggcacg aatctttagt
     3601 acttcgttga tcgccactcc accaagggtt tgccattaag cataactggc aatatttttg
     3661 tcaatttgcc ttttcgaccg tgtaaatgtc cctatccata ctctccgcag tcaatatccg
     3721 gaggtcaagt gtgaagactg tttaccccag caagatactt ggacaagcct ggtttgcaat
     3781 ccatagtttc agcaggcttc ctaggtggcc ttaagcctgg gatactggct ggctgctagc
     3841 aattgccatg actatgttgg aggaaatgtt cgagaggaat cttgggtcca tgaacagagt
     3901 agtcccactt caaatgttgc gtatgcctga tattacggac caatagagga acaacctggc
     3961 agcagctaac ttgtatatct ctaatcgcgt tgacgaccgt tctgtgtcag tgcaactgcc
     4021 acgcaaagac ttcagaataa taaactttgc cagagaaggc agttgtatta tgttaatatc
     4081 gcaacattgt gaaaattaaa tgaactaaca atcaaaaaga aagaaaatcc gaactttccc
     4141 tccgtattat ctaacccgtg tgcttttgca acgcaatcca actattccag ttgacgacat
     4201 taatccaagc cagcatagga agaataccaa aaaccttcag acagccgtac ccgcaagcct
     4261 tgttaccaac cagctatagc aatcttaccc gttcaatttc ttaatcccca tctagacgct
     4321 tccagcactc tgactgtcgg gagacgggtt cctttgccag ccatgagcat tcgcctagtt
     4381 agggagcggc ggatgcctac ttacttctgg gaacgaggcg cgccgtgagc gggaaatatg
     4441 ggtatcttgg gagaggagag ttggagtagg gagaaatgct ggctcggtgg gaggacgagg
     4501 gagctttaat aataccgctc tggctttggt agcactgagc cagtggtcgc agcaaaagag
     4561 gggaaaggag gggaacaggt caagcggagc cgctcttgtt gaggatagct gttacccatg
     4621 gaaattacat gtagcaagct agcaacccgc cgaagtgccc cactggtctt caagagccag
     4681 tccaggtgaa ctgcattccg tccccttcgc cgcggtgccc actgcctcca cagcctagat
     4741 gattctgaaa acacgttgaa gagctcgcga taataaccga ggcgggtaat gcaggatgaa
     4801 acttacgatc tacaacctac agcaccctgg ttctgaaaca aagttgagga tccccgcgat
     4861 cgataaccca gaccgtaatg cgaattgcaa tttacgattt accccccgta gagcggactg
     4921 attctggacc tatcttgaga ctatgtggtg gagcctgcga tgataaccga ggccgtaatg
     4981 caaaaatgaa aattacgaac caagaggcgt gatgagaaga tcagaattct gggctctgag
     5041 tgtgtttggt gttgccaaag acatgatctc ttactgagag ttattctgtg tctgacgaaa
     5101 tatgttgtgt atatatatat atgtacgtta aaagttccgt ggagttacca gtgattgacc
     5161 aatgttttat cttctacagt tctgcctgtc taccccattc tagctgtacc tgactacaga
     5221 gtagtttaat tgtggttgac cccacagtcg gaggcggagg aatacagcac cgatgtggcc
     5281 tgtctccatc cagattggca cgcaattttt acacgcggaa aagatcgaga tagagtacga
     5341 ctttaaattt agtccccggc ggcttctatt ttagaatatt tgagatttga ttctcaagca
     5401 attgatttgg ttgggtcacc ctcaattgga taatatacct cattgctcgg ctacttcaac
     5461 tcatcaatca ccgtcatacc ccgcatataa ccctccattc ccacgatgtc gtccaagtcg
     5521 caattgactt acggtgctcg agccagcaag caccccaatc ctctggcaaa gagacttttt
     5581 gagattgccg aagcaaagaa gacaaacgtt accgtctctg ctgatgtgac gacaacccga
     5641 gaactcctgg acctcgctga ccgtacggaa gctgttggat ccaatacata tgccgtctag
     5701 caatggacta atcaactttt gatgatacag gtctcggtcc ctacatcgcc gtcatcaaga
     5761 cacacatcga catcctcacc gatttcagcg tcgacactat caatggcctg aatgtgctgg
     5821 ctcaaaagca caactttttg atcttcgagg accgcaaatt catcgacatc ggcaataccg
     5881 tccagaagca ataccacggc ggtgctctga ggatctccga atgggcccac attatcaact
     5941 gcagcgttct ccctggcgag ggcatcgtcg aggctctggc ccagaccgca tctgcgcaag
     6001 acttccccta tggtcctgag agaggactgt tggtcctggc agagatgacc tccaaaggat
     6061 cgctggctac gggcgagtat accaaggcat cggttgacta cgctcgcaaa tacaagaact
     6121 tcgttatggg tttcgtgtcg acgcgggccc tgacggaagt gcagtcggat gtgtcttcag
     6181 cctcggagga tgaagatttc gtggtcttca cgacgggtgt gaacctctct tccaaaggag
     6241 ataagcttgg acagcaatac cagactcctg catcggctat tggacgcggt gccgacttta
     6301 tcatcgccgg tcgaggcatc tacgctgctc ccgacccggt tgaagctgca cagcggtacc
     6361 agaaagaagg ctgggaagct tatatggcac gtgtctgtgg taaaagctga actctgattg
     6421 ctagccagaa ctaccgttcg tataaagtat cctatacgaa gttatccctt aaggtatatg
     6481 cggcaagtca tgatttcctc ttggagcaaa agtgtagtgc cagtacgagt gttgtggagg
     6541 aaggctgcat acattgtgcc tgtcattaaa cgatgagctc gtccgtattg gcccctgtaa
     6601 tgccatgttt tccgccccca atcgtcaagg ttttcccttt gttagattcc taccagtcat
     6661 ctagcaagtg aggtaagctt tgccagaaac gccaaggctt tatctatgta gtcgataagc
     6721 aaagtggact gatagcttaa tatggaaggt ccctcaggac aagtcgacct gtgcagaaga
     6781 gataacagct tggcatcacg catcagtgcc tcctctcaga cagaataagt cgccataagt
     6841 tatcgaccga acctaggtag ggtatatttg gtgacgacaa tacctcccga cgatacctgg
     6901 attagacacc gtatgccgcg aattaacgcc ttccgaccaa gtcccttttg gtgatcgaca
     6961 ctttaggatc atctcctgcc agtcgcccag actgacggtg ttatggtggc gcgctatggc
     7021 tttcctagcc ggtcaagctc cgtgcactag agagcggaga aggaggggga gttgataggg
     7081 aatgatatct aggtagagaa gtactccaac cagagatatg cggatcaatg ctccggcagt
     7141 tgttagcata ccccaacctc gtttaagtat cctcttcttc catccatctt tatcattatt
     7201 tgtctatgtg catccctaga tcacgtcaat tatttcgata aatggatacg tgcactcggg
     7261 aatgcgtgat tggctcgact cgtgttctca gttcctcatt cccccccacg ttacattgac
     7321 aacggagtat tagcctatgg tcctgttcga taagcttgct ccatcgcctc cactacgtca
     7381 agcggagtat cagacatgag ctctacccca gatatcgcct gcttatcgct ggttgctttg
     7441 tcatgcaagt acagatcaca tcggcaattc tgataacaga tggtgccatt gtcccgtaat
     7501 caattgccca ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7561 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7621 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7681 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7741 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7801 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7861 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7921 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7981 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8041 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8101 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8161 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8221 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8281 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8341 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8401 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8461 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8521 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8581 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8641 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8701 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8761 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8821 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8881 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8941 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9001 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9061 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9121 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9181 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9241 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9301 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9361 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9421 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9481 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9541 gctatgacca tgattacgcc actgaacgaa gcatctgtgc ttcattttgt agaacaaaaa
     9601 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     9661 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
     9721 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     9781 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     9841 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     9901 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     9961 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
    10021 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
    10081 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
    10141 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
    10201 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
    10261 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
    10321 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
    10381 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
    10441 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
    10501 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
    10561 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
    10621 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
    10681 atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta
    10741 tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg
    10801 tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt
    10861 ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc
    10921 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10981 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    11041 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    11101 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    11161 accacctttt caattcatca tttttttttt attctttttt ttgatttcgg tttccttgaa
    11221 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
    11281 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
    11341 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat
    11401 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc
    11461 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg
    11521 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga
    11581 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt
    11641 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact
    11701 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg
    11761 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag
    11821 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta
    11881 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
    11941 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
    12001 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta
    12061 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
    12121 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
    12181 aaaactaa
//
