LOCUS       sequence-353785-        9223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1122
FEATURES             Location/Qualifiers
     source          1..9223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2161..2749
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2214..2234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2632..2643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2821..3054
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3132..3253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3169..3188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3223..3242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3293..3428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3448..3467)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3449..3467)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3477..3494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3477..3493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3486..3508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3635..4090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3722..3741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3932..3953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4116..4220
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4221..4289,4290..5081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4439..4458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5252..5840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5741..5760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5994..6011
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6128..6149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6164..6169,6170..6187,6188..6194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6202..6218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6207..6229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6226..6242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6226..6242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6261..6281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6263..6281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6309..6535
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6536..6716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6763..6888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7381..7614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7799..7843
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8074..8284
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8096..8292
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8143..8278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8324..8926
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8324..8343)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8820..8840
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8969..9086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtc
     1201 ggaagcgggc gaggagcagc ccatggagac gacgggcgcc accgagaacg gacatgaggc
     1261 cgtccccgaa ggcgagtcgc cggccggggc tggcacgggc gccgcggcgg gggctggagg
     1321 cgcgaccgcg gcgcccccga gcgggaatca gaacggcgcc gagggcgacc agatcaacgc
     1381 cagcaagaac gaggaggacg cgggaaaaat gttcgttggt ggcctgagct gggatactag
     1441 caaaaaagat ttaaaagact attttactaa atttggagag gtcgttgact gtacaataaa
     1501 aatggatccc aacactggac ggtcaagagg gtttgggttt atcctgttca aagatgcagc
     1561 cagtgtggag aaggtcctag accagaagga gcacaggctg gatggccgtg tcattgaccc
     1621 taaaaaggcc atggctatga agaaggaccc ggtgaagaaa atcttcgttg ggggtctgaa
     1681 tcctgaagcc actgaggaaa agatcaggga gtactttggc gagtttgggg agattgaggc
     1741 cattgaattg ccaatggatc caaagttgaa caaaagacga ggttttgtgt ttatcacctt
     1801 taaagaagaa gaacccgtga agaaggttct ggagaaaaag ttccatactg tcagtggaag
     1861 caagtgtgag atcaaggtgg cccagcccaa agaagtctat cagcagcagc agtatggctc
     1921 tgggggccgt ggaaaccgca accgagggaa ccgaggcagc ggaggtggtg gtggaggtgg
     1981 aggtcagggt agtacaaact acggcaagag ccagcgacgt ggtggccatc agaataacta
     2041 caagccatac tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     2101 tatatcttgt ggaaaggacg aggtagggtg ggtaaataaa cgacgacgcg ttaagtcgac
     2161 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2221 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2281 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2341 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2401 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2461 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2521 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2581 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2641 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2701 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     2761 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     2821 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     2881 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     2941 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3001 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     3061 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     3121 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3241 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     3301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3421 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     3481 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     3541 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     3601 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     3661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3781 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3841 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3901 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3961 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4021 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4081 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4141 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4201 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4261 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4321 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4381 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4441 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4501 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4561 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4621 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4681 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4741 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4801 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4861 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4921 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4981 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5041 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5101 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5161 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5221 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5281 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5341 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5401 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5461 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5521 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5581 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5641 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5701 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5761 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5821 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5881 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5941 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6001 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6061 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6241 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     6301 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     6361 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     6421 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     6481 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     6541 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6601 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6661 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     6721 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     6781 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     6841 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     6901 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     6961 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7021 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7081 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7141 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7201 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     7261 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7321 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7381 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     7441 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     7501 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     7561 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     7621 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     7681 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     7741 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     7801 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     7861 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     7921 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     7981 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
     8041 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
     8101 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8161 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8221 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8281 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
     8341 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     8401 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     8461 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     8521 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     8581 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     8641 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     8701 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     8761 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     8821 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     8881 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
     8941 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     9001 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     9061 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     9121 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
     9181 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
