LOCUS       sequence-353786-       14746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14746)
  AUTHORS   Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, 
            Karton A, Piggott AM, Chooi YH
  TITLE     Heterologous biosynthesis of elsinochrome A sheds light on the 
            formation of the photosensitive perylenequinone system.
  JOURNAL   Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. 
            eCollection 2019 Feb 7.
  PUBMED    30809363
REFERENCE   2  (bases 1 to 14746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c8sc02870b"; journalName: "Chem Sci"; date: "2018-11-22- 
            22"; volume: "10"; issue: "5"; pages: "1457-1465"
COMMENT     Sequence Label: pYFAC-riboB
FEATURES             Location/Qualifiers
     source          1..14746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2802..2823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             join(6830..6898,6899..7690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7048..7067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7861..8449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8350..8369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8603..8620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8737..8758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8773..8778,8779..8796,8797..8803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8811..8827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8816..8838
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8835..8851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8835..8851
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      8868..10210
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9831..9878)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10251..10269
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10307..10329)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10429..10448
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10472..10687
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10688..11491
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(11773..11790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11773..11789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11782..11804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12089..12108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      join(12130..14746,1..2632)
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
       61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
      121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
      181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
      241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
      301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
      361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
      421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
      481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
      541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
      601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
      661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
      721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
      781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
      841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
      901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
      961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
     1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
     1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
     1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
     1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
     1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
     1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
     1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
     1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
     1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
     1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
     1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
     1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
     1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
     1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
     1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
     1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
     1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
     2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
     2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
     2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
     2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
     2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
     2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
     2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
     2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
     2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
     2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
     3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
     3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
     3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
     3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
     3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
     3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
     3361 acgatggcat cctgcggccg ctggatgact ccctttcgat gatacccttt agcagaagtg
     3421 ttcttaaatt gcgggaagag gccgttcagg agtctggctc gtttgatcac atgggattaa
     3481 aatctggtgt tgaggctggg atattttatg ttttgtctct tgcgcttcga tatcaacaga
     3541 ttgacaataa tgaataacta tctacggact atgaatgcag ctcgatctac tccgtattta
     3601 gtcgggcaat ctcccgtaga agatgctaga ctgcgctgaa agactaggca cagttaattg
     3661 ccgcggatca cgtcaggcct gacaaatgac aggtggacct tcatgtaatc gcacgatgag
     3721 tcagcgcctc ttctcccccg ccgacttgca gctgcggatg tcccgaggag ccgaacattg
     3781 aatctatccc aagagtgact gaaatatgat tgcaatcgtc gtctttttct tgcagagaat
     3841 gtggtgaggt gatcaatcgt gtttttcttc atatattata ttctgtaatg atcatacttt
     3901 gttgagatac ccgtctctaa aattgtactt tcgcctccct cctccctctc aagtccgtca
     3961 tcgcaatgtc gaatttaccg tcccatgctt ctccgtcttt tggggcgcaa acgcctacca
     4021 cgccgcagcc cgtcgacgag ctttctctca agtcgaacat cacatcagcc cctgcgttac
     4081 actccagatt agacgaactc cgagatgaga cttccagcca gatcagcagc atagacactg
     4141 accgcgggga catgacccct gcggttcccg catcattact ctccccatcc ttcactcctc
     4201 cagcaacccc cgggggctcg atcaataccg cggagctgct tcaacagacc caattagctg
     4261 gcgcacggca agggtcaaca catactaagc ccccgaggtt gctttctcgt cttcccaatg
     4321 tcgaatgtat cgtccgcgct cggataccca cgacaactgg cgcggagatg tttcttcacc
     4381 tgtaccacaa tgacttagac aacaaagagc atttggcgat tgtgtttgga aatacaattc
     4441 gaagcagaag cttggacaag atccggcctg gagagacgga gatggatcgc atgatccgtg
     4501 gcgcgtatgt cgggaaactc cgaccgggcc gtgtgagcag ctggtatgac gaggagaaag
     4561 ctgaagaggc cgccagtccg gaagctggcg gcgctgtatc cagtccgcac acactgccgc
     4621 gaagtagcct caaggaggca cctcttgtga gaatccattc ggagtgctac actggtgaga
     4681 cggcttggtc tgcacgctgc gactgcggcg agcagctcga tgaagcagct cgcctcatgt
     4741 cgctgcctat ggaaacgttg gcagaggctg cgtcaccgcc ggatggggcg gtaccgtcaa
     4801 atgctgctgg aggtgtaatt gtgtacctgc gccaagaagg acgaggcatt gggctgggcg
     4861 agaaactgaa agcgtacaat cttcaggatc tgggatcaga cacggtggaa gccaatctcc
     4921 tgcttcgcca ccctgcggat gcaagaagtt atggattagc tacggcgatc ctggtggatc
     4981 ttggtctagg gattgactcg aatccgcacg gcatccgact actcacgaat aacccggaca
     5041 agattcgagc ggttgaggga cccaaccgag aagtggtggt gaaagagcgg gtgccgatgg
     5101 tgccattggc atggagatcg ggtggaaaga tgggaatcaa gagttccgag gtcgaagggt
     5161 atctgagaac gaaggtatgt cgacaatcta tctcttgcaa gctagcttat tttctcatca
     5221 ttcttgaaca atgtctcact ctttcctcgc agatatcgaa aatgggccat ttgctccaat
     5281 gaacctatcc cctttcggtt agtaaccttg tgctggaagt acgccttcct gcttttggac
     5341 ttagccaagt cgacacggtg tatgctcgtc acactcatgt aacggttctg cagcgcaaac
     5401 gttctggtca cgttattcgc ttttgtccat tttgtctttt tgtgcggtac ataaactaga
     5461 ggattagcgc actttgtaca tgatgttccc gacatgtcac attaggcctc gattggtcaa
     5521 aataatccct ctctaacctc tggttcgcat actcttaaat gcagctctag tctctagacg
     5581 gactagttcc tcctgcagtg gatgtctgac gagtgtctgc aaggaccgtt ttccgtgtcc
     5641 agttagttcc aatcacccgg atatcggcga gacgtcggcc gattgagatc actgagtcaa
     5701 tggcgttgag tggcaattaa tatatctgga tgcctagaat cctataaatt gggaaaacta
     5761 gagaccccgg tctatcgtgg tggcagtgcg tcattttttc ctcttggagc aaaagtgtag
     5821 tgccagtacg agtgttgtgg aggaaggctg catacattgt gcctgtcatt aaacgatgag
     5881 ctcgtccgta ttggcccctg taatgccatg ttttccgccc ccaatcgtca aggttttccc
     5941 tttgttagat tcctaccagt catctagcaa gtgaggtaag ctttgccaga aacgccaagg
     6001 ctttatctat gtagtcgata agcaaagtgg actgatagct taatatggaa ggtccctcag
     6061 gacaagtcga cctgtgcaga agagataaca gcttggcatc acgcatcagt gcctcctctc
     6121 agacagaata agtcgccata agttatcgac cgaacctagg tagggtatat ttggtgacga
     6181 caatacctcc cgacgatacc tggattagac accgtatgcc gcgaattaac gccttccgac
     6241 caagtccctt ttggtgatcg acactttagg atcatctcct gccagtcgcc cagactgacg
     6301 gtgttatggt ggcgcgctat ggctttccta gccggtcaag ctccgtgcac tagagagcgg
     6361 agaaggaggg ggagttgata gggaatgata tctaggtaga gaagtactcc aaccagagat
     6421 atgcggatca atgctccggc agttgttagc ataccccaac ctcgtttaag tatcctcttc
     6481 ttccatccat ctttatcatt atttgtctat gtgcatccct agatcacgtc aattatttcg
     6541 ataaatggat acgtgcactc gggaatgcgt gattggctcg tctcgtgttc tcagttcctc
     6601 attccccccc acgttacatt gacaacggag tattagccta tggtcctgtt cgataagctt
     6661 gctccatcgc ctccactacg tcaagcggag tatcagacat gagctctacc ccagatatcg
     6721 cctgcttatc gctggttgct ttgtcatgca agtacagatc acatcggcaa ttctgataac
     6781 agatggtgcc attgtcccgt aatcaattgc ccattgaaaa aggaagagta tgagtattca
     6841 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6901 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6961 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7021 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7081 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7141 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7201 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7261 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7321 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7381 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7441 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7501 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7561 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7621 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7681 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7741 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7801 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7861 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7921 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7981 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8041 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8101 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8161 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8221 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8461 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8521 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8581 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8641 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8701 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8761 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8821 ataacaattt cacacaggaa acagctatga ccatgattac gccactgaac gaagcatctg
     8881 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     8941 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
     9001 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     9061 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
     9121 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
     9181 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
     9241 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
     9301 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
     9361 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
     9421 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
     9481 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
     9541 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
     9601 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
     9661 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
     9721 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
     9781 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
     9841 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
     9901 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
     9961 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
    10021 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
    10081 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
    10141 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
    10201 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10261 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10321 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10381 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10441 agattgtact gagagtgcac cataccacct tttcaattca tcattttttt tttattcttt
    10501 tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga
    10561 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
    10621 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
    10681 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
    10741 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
    10801 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
    10861 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
    10921 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
    10981 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
    11041 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
    11101 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
    11161 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
    11221 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
    11281 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
    11341 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
    11401 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
    11461 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
    11521 actaaactca caaattagag cttcaattta attatatcag ttattaccct atgcggtgtg
    11581 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
    11641 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
    11701 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtggggtacc
    11761 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    11821 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    11881 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
    11941 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
    12001 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    12061 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    12121 acgttcgcca gcttattttt tgtatactgt tttgtgatag cacgaagttt ttccacggta
    12181 tcttgttaaa aatatatatt tgtggcgggc ttacctacat caaattaata agagactaat
    12241 tataaactaa acacacaagc aagctacttt agggtaaaag tttataaatg cttttgacgt
    12301 ataaacgttg cttgtattta ttattacaat taaaggtgga tagaaaacct agagactagt
    12361 tagaaactaa tctcaggttt gcgttaaact aaatcagagc ccgagaggtt aacagaacct
    12421 agaaggggac tagatatccg ggtagggaaa caaaaaaaaa aaacaagaca gccacatatt
    12481 agggagacta gttagaagct agttccagga ctaggaaaat aaaagacaat gataccacag
    12541 tctagttgac aactagatag attctagatt gaggccaaag tctctgagat ccaggttagt
    12601 tgcaactaat actagttagt atctagtctc ctataactct gaagctagaa taacttacta
    12661 ctattatcct caccactgtt cagctgcgca aacggagtga ttgcaaggtg ttcagagact
    12721 agttattgac tagtcagtga ctagcaataa ctaacaaggt attaacctac catgtctgcc
    12781 atcaccctgc acttcctcgg gctcagcagc cttttcctcc tcattttcat gctcattttc
    12841 cttgtttaag actgtgacta gtcaaagact agtccagaac cacaaaggag aaatgtctta
    12901 ccactttctt cattgcttgt ctcttttgca ttatccatgt ctgcaactag ttagagtcta
    12961 gttagtgact agtccgacga ggacttgctt gtctccggat tgttggagga actctccagg
    13021 gcctcaagat ccacaacaga gccttctaga agactggtca ataactagtt ggtctttgtc
    13081 tgagtctgac ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg atgagaaaaa
    13141 gcgccaaaac tcgcaatatg gctttgaacc acacggtgct gagactagtt agaatctagt
    13201 cccaaactag cttggatagc ttacctttgc cctttgcgtt gcgacaggtc ttgcagggta
    13261 tggttccttt ctcaccagct gatttagctg ccttgctacc ctcacggcgg atctgcataa
    13321 agagtggcta gaggttataa attagcactg atcctaggta cggggctgaa tgtaacttgc
    13381 ctttcctttc tcatcgcgcg gcaagacagg cttgctcaaa ttcctaccag tcacaggggt
    13441 atgcacggcg tacggaccac ttgaactagt cacagattag ttagcaacta gtctgcattg
    13501 aatggctgta cttacgggcc ctcgccattg tcctgatcat ttccagcttc accctcgttg
    13561 ctgcaaagta gttagtgact agtcaaggac tagttgaaat gggagaagaa actcacgaat
    13621 tctcgacacc cttagtattg tggtccttgg acttggtgct gctatatatt agctaataca
    13681 ctagttagac tcacagaaac ttacgcagct cgcttgcgct tcttggtagg agtcggggtt
    13741 gggagaacag tgccttcaaa caagccttca taccatgcta cttgactagt cagggactag
    13801 tcaccaagta atctagatag gacttgcctt tggcctccat cagttccttc atagtgggag
    13861 gtccattgtg caatgtaaac tccatgccgt gggagttctt gtccttcaag tgcttgacca
    13921 atatgtttct gttggcagag ggaacctgtc aactagttaa taactagtca gaaactagta
    13981 tagcagtaga ctcactgtac gcttgaggca tcccttcact cggcagtaga cttcatatgg
    14041 atggatatca ggcacgccat tgtcgtcctg tggactagtc agtaactagg cttaaagcta
    14101 gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc tccccgtcct taactgcctc
    14161 gagatagtga cagtactctg gggactttcg gagatcgtta tcgcgaatgc tcggcatact
    14221 aatcgttgac tagtcttgga ctagtcccga gcaaaaagga ttggaggagg aggaggaagg
    14281 tgagagtgag acaaagagcg aaataagagc ttcaaaggct atctctaagc agtatgaagg
    14341 ttaagtatct agttcttgac tagatttaaa agagatttcg actagttatg tacctggagt
    14401 ttggatatag gaatgtgttg tggtaacgaa atgtaagggg gaggaaagaa aaagtcggtc
    14461 aagaggtaac tctaagtcgg ccattccttt ttgggaggcg ctaaccataa acggcatggt
    14521 cgacttagag ttagctcagg gaatttaggg agttatctgc gaccactgga ctctcaggct
    14581 ttccattggg gtactaacat agccatcaaa tgccggcccg tgtttaatct cctcgagatg
    14641 gctgggctca catgcgttct actcaaaaac tcttatacct aggccctctc ccaatcgtgt
    14701 atgtttggac cttcctctct cacactttgg ataacatcaa gcatac
//
