LOCUS       sequence-353820-        2991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_16.10
FEATURES             Location/Qualifiers
     source          1..2991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        235..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(327..395,396..1187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1191..1230
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1341..1886
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1787..1806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2003..2020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2064..2150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2222..2256
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             2265..2276
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc
      361 cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga
      421 aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg
      481 gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag
      541 tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc
      601 gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga
      661 ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg
      721 cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata
      781 acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc
      841 cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc
      901 tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg
      961 ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata
     1021 aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta
     1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta
     1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta
     1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg
     1261 tctcgtacaa ttctgaggtc cacacagata gtgtccggcc ggccctagaa atattttatc
     1321 tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa
     1381 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg
     1441 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct
     1501 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag
     1561 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1621 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
     1681 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg
     1741 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt
     1801 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     1861 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt
     1921 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg
     1981 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatccggcg
     2041 cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     2101 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctttaa
     2161 ttaaggctcg ggagacctat cggtaataac agtccaatct ggtgtaactt cggaatcgtc
     2221 cttgacagct agctcagtcc taggtataat gctagctact agtgaaagag gagaaatact
     2281 agtatggtta gcaaaggcga ggcggttatc aaggagttta tgcgttttaa ggttcacatg
     2341 gagggtagca tgaatggtca cgagttcgag atcgagggtg aaggcgaggg tcgtccgtac
     2401 gaaggcaccc agaccgcgaa gctgaaagtg accaagggtg gcccgctgcc gttcagctgg
     2461 gacatcctga gcccgcagtt catgtatggc agccgtgcgt ttaccaaaca cccggcggac
     2521 attccggatt actataagca aagcttcccg gaaggtttta aatgggagcg tgttatgaac
     2581 ttcgaagatg gtggcgcggt gaccgttacc caggacacca gcctggagga tggcaccctg
     2641 atttacaagg tgaaactgcg tggcaccaac tttccgccgg atggtccggt tatgcagaag
     2701 aaaacgatgg gttgggaagc gagcaccgag cgtctgtatc cggaagatgg cgtgctgaag
     2761 ggtgatatca aaatggcgct gcgtctgaag gacggtggcc gttacctggc ggattttaag
     2821 accacctata aagcgaagaa accggtgcaa atgccgggtg cgtacaacgt tgaccgtaaa
     2881 ctggatatta ccagccacaa cgaggattat accgtggttg agcaatatga gcgtagcgag
     2941 ggtcgccaca gcaccggcgg catggacgaa ctgtataagg gatcctaata a
//
