LOCUS       sequence-353821-        5891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_45a.10
FEATURES             Location/Qualifiers
     source          1..5891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             398..1189
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1266..2117)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2104..2943)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3454..4425)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4532..4926
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(4964..5050)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5122..5156
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             5165..5176
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
      421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg
     1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccgtaggc
     1261 cggcctcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt
     1321 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg
     1381 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa
     1441 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg
     1501 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc
     1561 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg
     1621 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg
     1681 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg
     1741 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca
     1801 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc
     1861 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg
     1921 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg
     1981 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg
     2041 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg
     2101 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg
     2161 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt
     2221 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc
     2281 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag
     2341 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt
     2401 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg
     2461 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag
     2521 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc
     2581 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac
     2641 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc
     2701 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc
     2761 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc
     2821 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta
     2881 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc
     2941 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg
     3001 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc
     3061 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg
     3121 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata
     3181 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt
     3241 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt
     3301 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta
     3361 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc
     3421 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct
     3481 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac
     3541 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg
     3601 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact
     3661 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc
     3721 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg
     3781 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg
     3841 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc
     3901 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg
     3961 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc
     4021 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc
     4081 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag
     4141 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac
     4201 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc
     4261 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt
     4321 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc
     4381 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga
     4441 ttcccacaca ttatacgagc cggaagcata aagtgtagct agatccgaag gatgagccgg
     4501 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt
     4561 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg
     4621 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta
     4681 gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca
     4741 gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg
     4801 atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg
     4861 tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg
     4921 ggttcgtgac agttagggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga
     4981 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     5041 gttttatttg atgcctttaa ttaaggctcg ggagacctat cggtaataac agtccaatct
     5101 ggtgtaactt cggaatcgtc cttgacagct agctcagtcc taggtataat gctagctact
     5161 agtgaaagag gagaaatact agtatggtta gcaaaggcga ggcggttatc aaggagttta
     5221 tgcgttttaa ggttcacatg gagggtagca tgaatggtca cgagttcgag atcgagggtg
     5281 aaggcgaggg tcgtccgtac gaaggcaccc agaccgcgaa gctgaaagtg accaagggtg
     5341 gcccgctgcc gttcagctgg gacatcctga gcccgcagtt catgtatggc agccgtgcgt
     5401 ttaccaaaca cccggcggac attccggatt actataagca aagcttcccg gaaggtttta
     5461 aatgggagcg tgttatgaac ttcgaagatg gtggcgcggt gaccgttacc caggacacca
     5521 gcctggagga tggcaccctg atttacaagg tgaaactgcg tggcaccaac tttccgccgg
     5581 atggtccggt tatgcagaag aaaacgatgg gttgggaagc gagcaccgag cgtctgtatc
     5641 cggaagatgg cgtgctgaag ggtgatatca aaatggcgct gcgtctgaag gacggtggcc
     5701 gttacctggc ggattttaag accacctata aagcgaagaa accggtgcaa atgccgggtg
     5761 cgtacaacgt tgaccgtaaa ctggatatta ccagccacaa cgaggattat accgtggttg
     5821 agcaatatga gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa ctgtataagg
     5881 gatcctaata a
//
