LOCUS       sequence-353822-        2949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_46.10
FEATURES             Location/Qualifiers
     source          1..2949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             398..1189
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1299..1844
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1745..1764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1961..1978
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2022..2108)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2180..2214
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             2223..2234
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
      421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg
     1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg
     1261 ccctagaaat attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc
     1321 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc
     1381 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt
     1441 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt
     1501 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata
     1561 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     1621 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     1681 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     1741 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     1801 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     1861 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     1921 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     1981 agcggaatat atccggcgcg cccagctgtc tagggcggcg gatttgtcct actcaggaga
     2041 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     2101 tttatttgat gcctttaatt aaggctcggg agacctatcg gtaataacag tccaatctgg
     2161 tgtaacttcg gaatcgtcct tgacagctag ctcagtccta ggtataatgc tagctactag
     2221 tgaaagagga gaaatactag tatggttagc aaaggcgagg cggttatcaa ggagtttatg
     2281 cgttttaagg ttcacatgga gggtagcatg aatggtcacg agttcgagat cgagggtgaa
     2341 ggcgagggtc gtccgtacga aggcacccag accgcgaagc tgaaagtgac caagggtggc
     2401 ccgctgccgt tcagctggga catcctgagc ccgcagttca tgtatggcag ccgtgcgttt
     2461 accaaacacc cggcggacat tccggattac tataagcaaa gcttcccgga aggttttaaa
     2521 tgggagcgtg ttatgaactt cgaagatggt ggcgcggtga ccgttaccca ggacaccagc
     2581 ctggaggatg gcaccctgat ttacaaggtg aaactgcgtg gcaccaactt tccgccggat
     2641 ggtccggtta tgcagaagaa aacgatgggt tgggaagcga gcaccgagcg tctgtatccg
     2701 gaagatggcg tgctgaaggg tgatatcaaa atggcgctgc gtctgaagga cggtggccgt
     2761 tacctggcgg attttaagac cacctataaa gcgaagaaac cggtgcaaat gccgggtgcg
     2821 tacaacgttg accgtaaact ggatattacc agccacaacg aggattatac cgtggttgag
     2881 caatatgagc gtagcgaggg tcgccacagc accggcggca tggacgaact gtataaggga
     2941 tcctaataa
//
