LOCUS       sequence-353823-        4151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_5a7.10
FEATURES             Location/Qualifiers
     source          1..4151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             277..1467
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(629..648)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(1541..2491)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2539..2761
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3028..3114)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3278..3312
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        3315..3364
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3430..3438
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat ttgacagctt atcatcgata aactgtaatg cggtagttta
      241 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
      301 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
      361 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
      421 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
      481 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
      541 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc
      601 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      661 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      721 gtggccgggg gactgttggg cgccatctcc ctgcacgcac cattccttgc ggcggcggtg
      781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc
     1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     1201 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     1261 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc
     1501 gtacaattct gaggtccaca cagatagtgt ccggccggcc tcagatcctt ccgtatttag
     1561 ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga
     1621 gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt
     1681 ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg
     1741 taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta
     1801 cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc
     1861 gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca
     1921 aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact
     1981 taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt
     2041 ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca
     2101 gattatattt tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa
     2161 ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc
     2221 ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag
     2281 ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag
     2341 tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca
     2401 cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt
     2461 catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat
     2521 cactatacca attgagatgg gctagtcaat gataattaca tgtccttttc ctttgagttg
     2581 tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct
     2641 ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt
     2701 atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact
     2761 cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc
     2821 tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat
     2881 cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac
     2941 attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc
     3001 ggcgcgccca gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
     3061 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     3121 ttaattaagg ctcgggagac ctatcggtaa taacagtcca atctggtgta acttcggaat
     3181 cgtccccaat tattgaacac ccttcggggt gtttttttgt ttctggtcta ccatctcgtt
     3241 gtgataatag acctgaagtg cctactctgg aaaatctttg acagctagct cagtcctagg
     3301 tataatgcta gcagctgtca ccggatgtgc tttccggtct gatgagtccg tgaggacgaa
     3361 acagcctcta caaataattt tgtttaaggc tcgctacgag aagattgtta ctttcgcagc
     3421 gttattatct aaggaggtag tccatggtta gcaaaggcga ggcggttatc aaggagttta
     3481 tgcgttttaa ggttcacatg gagggtagca tgaatggtca cgagttcgag atcgagggtg
     3541 aaggcgaggg tcgtccgtac gaaggcaccc agaccgcgaa gctgaaagtg accaagggtg
     3601 gcccgctgcc gttcagctgg gacatcctga gcccgcagtt catgtatggc agccgtgcgt
     3661 ttaccaaaca cccggcggac attccggatt actataagca aagcttcccg gaaggtttta
     3721 aatgggagcg tgttatgaac ttcgaagatg gtggcgcggt gaccgttacc caggacacca
     3781 gcctggagga tggcaccctg atttacaagg tgaaactgcg tggcaccaac tttccgccgg
     3841 atggtccggt tatgcagaag aaaacgatgg gttgggaagc gagcaccgag cgtctgtatc
     3901 cggaagatgg cgtgctgaag ggtgatatca aaatggcgct gcgtctgaag gacggtggcc
     3961 gttacctggc ggattttaag accacctata aagcgaagaa accggtgcaa atgccgggtg
     4021 cgtacaacgt tgaccgtaaa ctggatatta ccagccacaa cgaggattat accgtggttg
     4081 agcaatatga gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa ctgtataagg
     4141 gatcctaata a
//
