LOCUS       sequence-353824-        3880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_5a9.10
FEATURES             Location/Qualifiers
     source          1..3880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             277..1467
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMSGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(629..648)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      1566..2154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2055..2074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2308..2325
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2340..2480
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2406..2425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2566..2588
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2582..2773)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2953..3039)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3111..3145
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             3154..3165
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttac gtatttaaat ttgacagctt atcatcgata aactgtaatg cggtagttta
      241 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
      301 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
      361 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
      421 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
      481 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
      541 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc
      601 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      661 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      721 gtggccgggg gactgttggg cgccatctcc ctgcacgcac cattccttgc ggcggcggtg
      781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc
     1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     1201 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     1261 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc
     1501 gtacaattct gaggtccaca cagatagtgt ccggccggcc ccgtagaaaa gatcaaagga
     1561 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1621 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1681 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1741 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1801 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1861 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1921 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1981 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2041 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2101 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2161 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2221 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2281 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2341 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     2401 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     2461 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2521 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2581 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     2641 cgtggtcgtg cagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     2701 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     2761 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
     2821 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     2881 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggggcgc gcccagctgt
     2941 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     3001 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat taaggctcgg
     3061 gagacctatc ggtaataaca gtccaatctg gtgtaacttc ggaatcgtcc tttacggcta
     3121 gctcagtcct aggtatagtg ctagctacta gtgaaagagg agaaatacta gtatggttag
     3181 caaaggcgag gcggttatca aggagtttat gcgttttaag gttcacatgg agggtagcat
     3241 gaatggtcac gagttcgaga tcgagggtga aggcgagggt cgtccgtacg aaggcaccca
     3301 gaccgcgaag ctgaaagtga ccaagggtgg cccgctgccg ttcagctggg acatcctgag
     3361 cccgcagttc atgtatggca gccgtgcgtt taccaaacac ccggcggaca ttccggatta
     3421 ctataagcaa agcttcccgg aaggttttaa atgggagcgt gttatgaact tcgaagatgg
     3481 tggcgcggtg accgttaccc aggacaccag cctggaggat ggcaccctga tttacaaggt
     3541 gaaactgcgt ggcaccaact ttccgccgga tggtccggtt atgcagaaga aaacgatggg
     3601 ttgggaagcg agcaccgagc gtctgtatcc ggaagatggc gtgctgaagg gtgatatcaa
     3661 aatggcgctg cgtctgaagg acggtggccg ttacctggcg gattttaaga ccacctataa
     3721 agcgaagaaa ccggtgcaaa tgccgggtgc gtacaacgtt gaccgtaaac tggatattac
     3781 cagccacaac gaggattata ccgtggttga gcaatatgag cgtagcgagg gtcgccacag
     3841 caccggcggc atggacgaac tgtataaggg atcctaataa
//
