LOCUS       sequence-353825-        3915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_17.10
FEATURES             Location/Qualifiers
     source          1..3915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        235..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(327..395,396..1187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1191..1230
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(1305..2255)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2303..2525
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2792..2878)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3042..3076
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        3079..3128
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3194..3202
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc
      361 cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga
      421 aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg
      481 gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag
      541 tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc
      601 gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga
      661 ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg
      721 cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata
      781 acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc
      841 cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc
      901 tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg
      961 ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata
     1021 aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta
     1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta
     1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta
     1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg
     1261 tctcgtacaa ttctgaggtc cacacagata gtgtccggcc ggcctcagat ccttccgtat
     1321 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     1381 ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     1441 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct
     1501 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     1561 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     1621 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     1681 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     1741 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     1801 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     1861 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     1921 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     1981 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     2041 ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta
     2101 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     2161 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     2221 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     2281 taatcactat accaattgag atgggctagt caatgataat tacatgtcct tttcctttga
     2341 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     2401 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     2461 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     2521 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     2581 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     2641 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     2701 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     2761 gcgcggcgcg cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     2821 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     2881 gcctttaatt aaggctcggg agacctatcg gtaataacag tccaatctgg tgtaacttcg
     2941 gaatcgtccc caattattga acacccttcg gggtgttttt ttgtttctgg tctaccatct
     3001 cgttgtgata atagacctga agtgcctact ctggaaaatc tttgacagct agctcagtcc
     3061 taggtataat gctagcagct gtcaccggat gtgctttccg gtctgatgag tccgtgagga
     3121 cgaaacagcc tctacaaata attttgttta aggctcgcta cgagaagatt gttactttcg
     3181 cagcgttatt atctaaggag gtagtccatg gttagcaaag gcgaggcggt tatcaaggag
     3241 tttatgcgtt ttaaggttca catggagggt agcatgaatg gtcacgagtt cgagatcgag
     3301 ggtgaaggcg agggtcgtcc gtacgaaggc acccagaccg cgaagctgaa agtgaccaag
     3361 ggtggcccgc tgccgttcag ctgggacatc ctgagcccgc agttcatgta tggcagccgt
     3421 gcgtttacca aacacccggc ggacattccg gattactata agcaaagctt cccggaaggt
     3481 tttaaatggg agcgtgttat gaacttcgaa gatggtggcg cggtgaccgt tacccaggac
     3541 accagcctgg aggatggcac cctgatttac aaggtgaaac tgcgtggcac caactttccg
     3601 ccggatggtc cggttatgca gaagaaaacg atgggttggg aagcgagcac cgagcgtctg
     3661 tatccggaag atggcgtgct gaagggtgat atcaaaatgg cgctgcgtct gaaggacggt
     3721 ggccgttacc tggcggattt taagaccacc tataaagcga agaaaccggt gcaaatgccg
     3781 ggtgcgtaca acgttgaccg taaactggat attaccagcc acaacgagga ttataccgtg
     3841 gttgagcaat atgagcgtag cgagggtcgc cacagcaccg gcggcatgga cgaactgtat
     3901 aagggatcct aataa
//
