LOCUS       sequence-353827-        3644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_19.10
FEATURES             Location/Qualifiers
     source          1..3644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        235..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(327..395,396..1187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1191..1230
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1330..1918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1819..1838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2072..2089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2104..2244
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2170..2189)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2330..2352
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2346..2537)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2717..2803)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2875..2909
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2918..2929
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc
      361 cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga
      421 aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg
      481 gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag
      541 tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc
      601 gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga
      661 ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg
      721 cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata
      781 acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc
      841 cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc
      901 tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg
      961 ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata
     1021 aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta
     1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta
     1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta
     1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg
     1261 tctcgtacaa ttctgaggtc cacacagata gtgtccggcc ggccccgtag aaaagatcaa
     1321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2101 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     2161 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     2221 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     2281 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     2341 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     2401 tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     2461 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     2521 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     2581 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     2641 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcgggg gcgcgcccag
     2701 ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     2761 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctt taattaaggc
     2821 tcgggagacc tatcggtaat aacagtccaa tctggtgtaa cttcggaatc gtcctttacg
     2881 gctagctcag tcctaggtat agtgctagct actagtgaaa gaggagaaat actagtatgg
     2941 ttagcaaagg cgaggcggtt atcaaggagt ttatgcgttt taaggttcac atggagggta
     3001 gcatgaatgg tcacgagttc gagatcgagg gtgaaggcga gggtcgtccg tacgaaggca
     3061 cccagaccgc gaagctgaaa gtgaccaagg gtggcccgct gccgttcagc tgggacatcc
     3121 tgagcccgca gttcatgtat ggcagccgtg cgtttaccaa acacccggcg gacattccgg
     3181 attactataa gcaaagcttc ccggaaggtt ttaaatggga gcgtgttatg aacttcgaag
     3241 atggtggcgc ggtgaccgtt acccaggaca ccagcctgga ggatggcacc ctgatttaca
     3301 aggtgaaact gcgtggcacc aactttccgc cggatggtcc ggttatgcag aagaaaacga
     3361 tgggttggga agcgagcacc gagcgtctgt atccggaaga tggcgtgctg aagggtgata
     3421 tcaaaatggc gctgcgtctg aaggacggtg gccgttacct ggcggatttt aagaccacct
     3481 ataaagcgaa gaaaccggtg caaatgccgg gtgcgtacaa cgttgaccgt aaactggata
     3541 ttaccagcca caacgaggat tataccgtgg ttgagcaata tgagcgtagc gagggtcgcc
     3601 acagcaccgg cggcatggac gaactgtata agggatccta ataa
//
