LOCUS       sequence-353828-        3659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_37.10
FEATURES             Location/Qualifiers
     source          1..3659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        210..312
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             313..972
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(379..398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(1049..1999)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2047..2269
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2536..2622)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2786..2820
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        2823..2872
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             2938..2946
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga
     1021 ggtccacaca gatagtgtcc ggccggcctc agatccttcc gtatttagcc agtatgttct
     1081 ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatacttac
     1141 tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag
     1201 catcgtgtag tgtttttctt agtccgttat gtaggtagga atctgatgta atggttgttg
     1261 gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt
     1321 gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca
     1381 ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt
     1441 taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa
     1501 ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact
     1561 cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta
     1621 tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact aattctaatt
     1681 tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag
     1741 gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat
     1801 aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg
     1861 tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa
     1921 ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga
     1981 aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat
     2041 tgagatgggc tagtcaatga taattacatg tccttttcct ttgagttgtg ggtatctgta
     2101 aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg
     2161 ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga
     2221 aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt
     2281 cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac
     2341 cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc
     2401 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
     2461 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcgg cgcgcccagc
     2521 tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     2581 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgccttt aattaaggct
     2641 cgggagacct atcggtaata acagtccaat ctggtgtaac ttcggaatcg tccccaatta
     2701 ttgaacaccc ttcggggtgt ttttttgttt ctggtctacc atctcgttgt gataatagac
     2761 ctgaagtgcc tactctggaa aatctttgac agctagctca gtcctaggta taatgctagc
     2821 agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca
     2881 aataattttg tttaaggctc gctacgagaa gattgttact ttcgcagcgt tattatctaa
     2941 ggaggtagtc catggttagc aaaggcgagg cggttatcaa ggagtttatg cgttttaagg
     3001 ttcacatgga gggtagcatg aatggtcacg agttcgagat cgagggtgaa ggcgagggtc
     3061 gtccgtacga aggcacccag accgcgaagc tgaaagtgac caagggtggc ccgctgccgt
     3121 tcagctggga catcctgagc ccgcagttca tgtatggcag ccgtgcgttt accaaacacc
     3181 cggcggacat tccggattac tataagcaaa gcttcccgga aggttttaaa tgggagcgtg
     3241 ttatgaactt cgaagatggt ggcgcggtga ccgttaccca ggacaccagc ctggaggatg
     3301 gcaccctgat ttacaaggtg aaactgcgtg gcaccaactt tccgccggat ggtccggtta
     3361 tgcagaagaa aacgatgggt tgggaagcga gcaccgagcg tctgtatccg gaagatggcg
     3421 tgctgaaggg tgatatcaaa atggcgctgc gtctgaagga cggtggccgt tacctggcgg
     3481 attttaagac cacctataaa gcgaagaaac cggtgcaaat gccgggtgcg tacaacgttg
     3541 accgtaaact ggatattacc agccacaacg aggattatac cgtggttgag caatatgagc
     3601 gtagcgaggg tcgccacagc accggcggca tggacgaact gtataaggga tcctaataa
//
