LOCUS       sequence-353833-        5677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_35a.10
FEATURES             Location/Qualifiers
     source          1..5677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        210..312
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             313..972
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(379..398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(1052..1903)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1890..2729)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3240..4211)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4318..4712
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(4750..4836)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        4908..4942
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             4951..4962
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga
     1021 ggtccacaca gatagtgtcc gtaggccggc ctcagcctgc cgccttgggc cgggtgatgt
     1081 cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg
     1141 cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga
     1201 catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac
     1261 acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc
     1321 tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca
     1381 cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc
     1441 gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta
     1501 tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat
     1561 agctaccttt gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca
     1621 gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc
     1681 cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc
     1741 cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct
     1801 tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt
     1861 gccggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc
     1921 ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac
     1981 cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg
     2041 cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag
     2101 gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg
     2161 cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg
     2221 gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt
     2281 tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg
     2341 gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc
     2401 ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca
     2461 agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag gtagatcacc
     2521 gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc
     2581 agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta
     2641 ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc
     2701 agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt
     2761 aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc
     2821 gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct
     2881 tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg
     2941 tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc
     3001 ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg
     3061 actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa
     3121 tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata
     3181 aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac
     3241 tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt
     3301 gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc
     3361 tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc
     3421 atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata
     3481 aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg
     3541 tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg
     3601 gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc
     3661 tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac
     3721 ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg
     3781 ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg
     3841 gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag
     3901 ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc
     3961 aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc
     4021 ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc
     4081 acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg
     4141 atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg
     4201 tcgttcttca tcgggccacc aagcgattcc cacacattat acgagccgga agcataaagt
     4261 gtagctagat ccgaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc
     4321 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct
     4381 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct
     4441 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct
     4501 ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct
     4561 agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc
     4621 ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg
     4681 caggggggcg tgacagttat tgcaggggtt cgtgacagtt agggcgcgcc cagctgtcta
     4741 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4801 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctttaattaa ggctcgggag
     4861 acctatcggt aataacagtc caatctggtg taacttcgga atcgtccttg acagctagct
     4921 cagtcctagg tataatgcta gctactagtg aaagaggaga aatactagta tggttagcaa
     4981 aggcgaggcg gttatcaagg agtttatgcg ttttaaggtt cacatggagg gtagcatgaa
     5041 tggtcacgag ttcgagatcg agggtgaagg cgagggtcgt ccgtacgaag gcacccagac
     5101 cgcgaagctg aaagtgacca agggtggccc gctgccgttc agctgggaca tcctgagccc
     5161 gcagttcatg tatggcagcc gtgcgtttac caaacacccg gcggacattc cggattacta
     5221 taagcaaagc ttcccggaag gttttaaatg ggagcgtgtt atgaacttcg aagatggtgg
     5281 cgcggtgacc gttacccagg acaccagcct ggaggatggc accctgattt acaaggtgaa
     5341 actgcgtggc accaactttc cgccggatgg tccggttatg cagaagaaaa cgatgggttg
     5401 ggaagcgagc accgagcgtc tgtatccgga agatggcgtg ctgaagggtg atatcaaaat
     5461 ggcgctgcgt ctgaaggacg gtggccgtta cctggcggat tttaagacca cctataaagc
     5521 gaagaaaccg gtgcaaatgc cgggtgcgta caacgttgac cgtaaactgg atattaccag
     5581 ccacaacgag gattataccg tggttgagca atatgagcgt agcgagggtc gccacagcac
     5641 cggcggcatg gacgaactgt ataagggatc ctaataa
//
