LOCUS       sequence-353834-        3388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_39.10
FEATURES             Location/Qualifiers
     source          1..3388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        210..312
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             313..972
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(379..398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1074..1662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1563..1582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1816..1833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1848..1988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1914..1933)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2074..2096
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2090..2281)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2461..2547)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2619..2653
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2662..2673
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga
     1021 ggtccacaca gatagtgtcc ggccggcccc gtagaaaaga tcaaaggatc ttcttgagat
     1081 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1141 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1201 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1261 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1321 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1381 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1441 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1501 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1561 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1621 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1681 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1741 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1801 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     1861 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     1921 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     1981 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     2041 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     2101 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca
     2161 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     2221 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     2281 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     2341 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     2401 agggtaaaca actggcggta tggatgcggc gggggcgcgc ccagctgtct agggcggcgg
     2461 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     2521 tttcgactga gcctttcgtt ttatttgatg cctttaatta aggctcggga gacctatcgg
     2581 taataacagt ccaatctggt gtaacttcgg aatcgtcctt tacggctagc tcagtcctag
     2641 gtatagtgct agctactagt gaaagaggag aaatactagt atggttagca aaggcgaggc
     2701 ggttatcaag gagtttatgc gttttaaggt tcacatggag ggtagcatga atggtcacga
     2761 gttcgagatc gagggtgaag gcgagggtcg tccgtacgaa ggcacccaga ccgcgaagct
     2821 gaaagtgacc aagggtggcc cgctgccgtt cagctgggac atcctgagcc cgcagttcat
     2881 gtatggcagc cgtgcgttta ccaaacaccc ggcggacatt ccggattact ataagcaaag
     2941 cttcccggaa ggttttaaat gggagcgtgt tatgaacttc gaagatggtg gcgcggtgac
     3001 cgttacccag gacaccagcc tggaggatgg caccctgatt tacaaggtga aactgcgtgg
     3061 caccaacttt ccgccggatg gtccggttat gcagaagaaa acgatgggtt gggaagcgag
     3121 caccgagcgt ctgtatccgg aagatggcgt gctgaagggt gatatcaaaa tggcgctgcg
     3181 tctgaaggac ggtggccgtt acctggcgga ttttaagacc acctataaag cgaagaaacc
     3241 ggtgcaaatg ccgggtgcgt acaacgttga ccgtaaactg gatattacca gccacaacga
     3301 ggattatacc gtggttgagc aatatgagcg tagcgagggt cgccacagca ccggcggcat
     3361 ggacgaactg tataagggat cctaataa
//
