LOCUS       sequence-353836-        2735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_36.10
FEATURES             Location/Qualifiers
     source          1..2735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        210..312
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             313..972
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(379..398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1085..1630
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1531..1550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1747..1764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1808..1894)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1966..2000
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             2009..2020
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga
     1021 ggtccacaca gatagtgtcc ggccggccct agaaatattt tatctgatta ataagatgat
     1081 cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct
     1141 tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc
     1201 ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact
     1261 tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt
     1321 ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg
     1381 gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg
     1441 cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg
     1501 aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt
     1561 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag
     1621 cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca
     1681 tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc
     1741 gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc ggcgcgccca gctgtctagg
     1801 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     1861 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ttaattaagg ctcgggagac
     1921 ctatcggtaa taacagtcca atctggtgta acttcggaat cgtccttgac agctagctca
     1981 gtcctaggta taatgctagc tactagtgaa agaggagaaa tactagtatg gttagcaaag
     2041 gcgaggcggt tatcaaggag tttatgcgtt ttaaggttca catggagggt agcatgaatg
     2101 gtcacgagtt cgagatcgag ggtgaaggcg agggtcgtcc gtacgaaggc acccagaccg
     2161 cgaagctgaa agtgaccaag ggtggcccgc tgccgttcag ctgggacatc ctgagcccgc
     2221 agttcatgta tggcagccgt gcgtttacca aacacccggc ggacattccg gattactata
     2281 agcaaagctt cccggaaggt tttaaatggg agcgtgttat gaacttcgaa gatggtggcg
     2341 cggtgaccgt tacccaggac accagcctgg aggatggcac cctgatttac aaggtgaaac
     2401 tgcgtggcac caactttccg ccggatggtc cggttatgca gaagaaaacg atgggttggg
     2461 aagcgagcac cgagcgtctg tatccggaag atggcgtgct gaagggtgat atcaaaatgg
     2521 cgctgcgtct gaaggacggt ggccgttacc tggcggattt taagaccacc tataaagcga
     2581 agaaaccggt gcaaatgccg ggtgcgtaca acgttgaccg taaactggat attaccagcc
     2641 acaacgagga ttataccgtg gttgagcaat atgagcgtag cgagggtcgc cacagcaccg
     2701 gcggcatgga cgaactgtat aagggatcct aataa
//
