LOCUS       sequence-353837-        5821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_25a.10
FEATURES             Location/Qualifiers
     source          1..5821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(283..302)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             294..1109
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1196..2047)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2034..2873)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3384..4355)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      4462..4856
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(4894..4980)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5052..5086
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             5095..5106
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      241 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      301 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
      361 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
      421 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
      481 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
      541 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
      601 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
      661 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
      721 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
      781 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
      841 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
      901 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
      961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca
     1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccgtaggc cggcctcagc
     1201 ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt accgtccagc
     1261 ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg cgcatggtcg
     1321 aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa gccttgccgg
     1381 ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg gtttcgctgt
     1441 ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc atgacggcct
     1501 gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg ctggcgtact
     1561 ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg gataccttcc
     1621 aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg acctctgccc
     1681 cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca gcggtgacgg
     1741 cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc ttgggttccg
     1801 ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg atgcgcagat
     1861 catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg ccgtagtcat
     1921 acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg aacaggccgg
     1981 gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg ttcttaggct
     2041 tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg cttgagcacc
     2101 ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt ggccttgctc
     2161 acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc ctcggcctcg
     2221 gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag taccgagctg
     2281 ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt gctggcatgg
     2341 tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg accgatgacc
     2401 tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag cgtgtccagc
     2461 accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc cggggccatg
     2521 atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac ggcttgccgt
     2581 tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc ggtgggcggg
     2641 tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc cagcagatcc
     2701 ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc ggcaccaccg
     2761 ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta gtccagcggt
     2821 ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc cattgattgc
     2881 ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg ccctgcgccg
     2941 ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc agccagaact
     3001 tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg cgtacagcgt
     3061 cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata tcagtcaccg
     3121 agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt gcagccgtca
     3181 aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt gtttgacgta
     3241 taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta gaccgaccct
     3301 gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc atagtataac
     3361 aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct gtccatgcct
     3421 cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac gctgggcaga
     3481 cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg cttgcgctct
     3541 gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact gcggccccgg
     3601 ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc gaagcgcttg
     3661 accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg gcggctaagc
     3721 tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg ccgctgggcc
     3781 tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc ggtcttgccc
     3841 ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg cttgtccttg
     3901 cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc cgggtcggcg
     3961 tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc tgcggcgtcg
     4021 gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag ggcaggctcc
     4081 cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac cagcaccaga
     4141 ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc attcatccgc
     4201 ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt catcatctgg
     4261 ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc cttgagctgt
     4321 cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga ttcccacaca
     4381 ttatacgagc cggaagcata aagtgtagct agatccgaag gatgagccgg gctgaatgat
     4441 cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt agggggaaag
     4501 gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg tggggtttag
     4561 cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta gcctagcgca
     4621 gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca gcagcgcccc
     4681 acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg atggattttt
     4741 ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg tgacagttat
     4801 tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg ggttcgtgac
     4861 agttagggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac
     4921 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     4981 atgcctttaa ttaaggctcg ggagacctat cggtaataac agtccaatct ggtgtaactt
     5041 cggaatcgtc cttgacagct agctcagtcc taggtataat gctagctact agtgaaagag
     5101 gagaaatact agtatggtta gcaaaggcga ggcggttatc aaggagttta tgcgttttaa
     5161 ggttcacatg gagggtagca tgaatggtca cgagttcgag atcgagggtg aaggcgaggg
     5221 tcgtccgtac gaaggcaccc agaccgcgaa gctgaaagtg accaagggtg gcccgctgcc
     5281 gttcagctgg gacatcctga gcccgcagtt catgtatggc agccgtgcgt ttaccaaaca
     5341 cccggcggac attccggatt actataagca aagcttcccg gaaggtttta aatgggagcg
     5401 tgttatgaac ttcgaagatg gtggcgcggt gaccgttacc caggacacca gcctggagga
     5461 tggcaccctg atttacaagg tgaaactgcg tggcaccaac tttccgccgg atggtccggt
     5521 tatgcagaag aaaacgatgg gttgggaagc gagcaccgag cgtctgtatc cggaagatgg
     5581 cgtgctgaag ggtgatatca aaatggcgct gcgtctgaag gacggtggcc gttacctggc
     5641 ggattttaag accacctata aagcgaagaa accggtgcaa atgccgggtg cgtacaacgt
     5701 tgaccgtaaa ctggatatta ccagccacaa cgaggattat accgtggttg agcaatatga
     5761 gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa ctgtataagg gatcctaata
     5821 a
//
