LOCUS       sequence-353841-        3803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_27.10
FEATURES             Location/Qualifiers
     source          1..3803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(283..302)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             294..1109
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1193..2143)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2191..2413
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2680..2766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2930..2964
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        2967..3016
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3082..3090
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      241 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      301 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
      361 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
      421 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
      481 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
      541 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
      601 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
      661 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
      721 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
      781 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
      841 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
      901 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
      961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca
     1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg cctcagatcc
     1201 ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga
     1261 acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc tcaaaactgg
     1321 tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt
     1381 aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct
     1441 caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag
     1501 tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac
     1561 ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca
     1621 ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt
     1681 gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact
     1741 taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct
     1801 cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga
     1861 aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc
     1921 tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt
     1981 attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt
     2041 tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac
     2101 taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta
     2161 ggtgatttta atcactatac caattgagat gggctagtca atgataatta catgtccttt
     2221 tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt
     2281 ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag
     2341 tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc
     2401 ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc
     2461 tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa
     2521 gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt
     2581 ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg
     2641 cactacaggc gcggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     2701 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     2761 tatttgatgc ctttaattaa ggctcgggag acctatcggt aataacagtc caatctggtg
     2821 taacttcgga atcgtcccca attattgaac acccttcggg gtgttttttt gtttctggtc
     2881 taccatctcg ttgtgataat agacctgaag tgcctactct ggaaaatctt tgacagctag
     2941 ctcagtccta ggtataatgc tagcagctgt caccggatgt gctttccggt ctgatgagtc
     3001 cgtgaggacg aaacagcctc tacaaataat tttgtttaag gctcgctacg agaagattgt
     3061 tactttcgca gcgttattat ctaaggaggt agtccatggt tagcaaaggc gaggcggtta
     3121 tcaaggagtt tatgcgtttt aaggttcaca tggagggtag catgaatggt cacgagttcg
     3181 agatcgaggg tgaaggcgag ggtcgtccgt acgaaggcac ccagaccgcg aagctgaaag
     3241 tgaccaaggg tggcccgctg ccgttcagct gggacatcct gagcccgcag ttcatgtatg
     3301 gcagccgtgc gtttaccaaa cacccggcgg acattccgga ttactataag caaagcttcc
     3361 cggaaggttt taaatgggag cgtgttatga acttcgaaga tggtggcgcg gtgaccgtta
     3421 cccaggacac cagcctggag gatggcaccc tgatttacaa ggtgaaactg cgtggcacca
     3481 actttccgcc ggatggtccg gttatgcaga agaaaacgat gggttgggaa gcgagcaccg
     3541 agcgtctgta tccggaagat ggcgtgctga agggtgatat caaaatggcg ctgcgtctga
     3601 aggacggtgg ccgttacctg gcggatttta agaccaccta taaagcgaag aaaccggtgc
     3661 aaatgccggg tgcgtacaac gttgaccgta aactggatat taccagccac aacgaggatt
     3721 ataccgtggt tgagcaatat gagcgtagcg agggtcgcca cagcaccggc ggcatggacg
     3781 aactgtataa gggatcctaa taa
//
