LOCUS       sequence-353842-        3532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_29.10
FEATURES             Location/Qualifiers
     source          1..3532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(283..302)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             294..1109
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1218..1806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1707..1726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1960..1977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1992..2132
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2058..2077)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2218..2240
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2234..2425)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(2605..2691)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2763..2797
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2806..2817
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      241 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      301 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
      361 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
      421 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
      481 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
      541 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
      601 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
      661 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
      721 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
      781 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
      841 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
      901 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
      961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca
     1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg ccccgtagaa
     1201 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1261 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     1381 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1441 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1501 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1621 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1681 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1741 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1801 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1861 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1921 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1981 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2041 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     2101 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     2161 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2221 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     2281 aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca
     2341 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     2401 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     2461 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     2521 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggggc
     2581 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     2641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccttta
     2701 attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact tcggaatcgt
     2761 cctttacggc tagctcagtc ctaggtatag tgctagctac tagtgaaaga ggagaaatac
     2821 tagtatggtt agcaaaggcg aggcggttat caaggagttt atgcgtttta aggttcacat
     2881 ggagggtagc atgaatggtc acgagttcga gatcgagggt gaaggcgagg gtcgtccgta
     2941 cgaaggcacc cagaccgcga agctgaaagt gaccaagggt ggcccgctgc cgttcagctg
     3001 ggacatcctg agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac acccggcgga
     3061 cattccggat tactataagc aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa
     3121 cttcgaagat ggtggcgcgg tgaccgttac ccaggacacc agcctggagg atggcaccct
     3181 gatttacaag gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa
     3241 gaaaacgatg ggttgggaag cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa
     3301 gggtgatatc aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg cggattttaa
     3361 gaccacctat aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa
     3421 actggatatt accagccaca acgaggatta taccgtggtt gagcaatatg agcgtagcga
     3481 gggtcgccac agcaccggcg gcatggacga actgtataag ggatcctaat aa
//
